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Responses in a coculture of macrophages, immature dendritic cells and apoptotic cells

Research Project

Project/Area Number 16590068
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionToho University

Principal Investigator

KOBAYASHI Yoshiro  Toho University, Fac.of Sci, Professor, 理学部, 教授 (10134610)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsimmature dendritic cells / apoptotic cells / macrophages / neutrophil / chemokine / class II / 共培養 / 貪食 / サイトカイン
Research Abstract

We examined the effect of the addition of immature dendritic cells (iDCs) to a coculture of macrophages and apoptotic CTLL-2 cells on phagocytosis by macrophages and MIP-2 production. The more apoptotic cells macrophages phagocytosed, the more MIP-2 was produced by a coculture of macrophages and apoptotic cells. The addition of iDCs to the coculture, however, enhanced the phagocytosis by macrophages but reduced MIP-2 production. Further study revealed that the reduction of MIP-2 production was dependent on cell-to-cell contact partly involving β_2 integrin family Mac-1 as well as anti-inflammatory cytokine, IL-10 and TGF-β. Both cytokines were expressed by iDCs more significantly than macrophages at the mRNA levels, although they were hardly detected in the supernatant at the protein levels. On the other hand, while coculturing iDCs with apoptotic cells produced IL-6, the addition of macrophages to the coculture decreased phagocytosis by iDCs and IL-6 production, suggesting that reduction of IL-6 is caused by the decrease in phagocytosis. Thus this study reveals a new mechanism by which MIP-2 production by macrophages phagocytosing apoptotic cells is prevented. We then examined the effect of the addition of neutrophils to a cocultue of iDCs and apoptotic cells on class II expression and MIP-2 and KC production. The Class II expression and KC production but not MIP-2 production were significantly inhibited by the addition of neutrophils.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (18 results)

All 2005 2004 Other

All Journal Article (18 results)

  • [Journal Article] Annexins I and IV inhibit Staphylococcus aureus attachment to human macrophages.2005

    • Author(s)
      Gotoh, M. et al.
    • Journal Title

      Immunology Letters 98

      Pages: 297-302

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] A novel accessory role of neutrophils in Concanavalin A-induced hepatitis.2005

    • Author(s)
      Hatada, S. et al.
    • Journal Title

      Cell. Immunol. 233

      Pages: 23-29

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Neutrophils accelerate macrophage-mediated digestion of apoptotic cells in vivo as well as in vitro.2005

    • Author(s)
      Iyoda, T. et al.
    • Journal Title

      J. Immunol. 175

      Pages: 3475-3483

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Inhibition by adiponectin of IL-8 production by human macrophages upon coculturing with late apoptotic cells.2005

    • Author(s)
      Saijo, S. et al.
    • Journal Title

      Biochem. Biophys. Res Commun. 334

      Pages: 1180-1183

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Annexins I and IV inhibit Staphylococcus aureus attachment to human macrophages.2005

    • Author(s)
      Gotoh M., Takamoto Y., Kurosaka K., Masuda J., Ida M., Satoh A., Takayama E., Kojima-Aikawa K., Kobayashi Y., Matsumoto I.
    • Journal Title

      Immunology Letters 98

      Pages: 297-302

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A novel accessory role of neutrophils in Concanavalin A-induced hepatitis.2005

    • Author(s)
      Hatada, S., Ohta, T.Shiratsuchi, Y., Hatano, M., Kobayashi, Y.
    • Journal Title

      Cell.Immunol. 233

      Pages: 23-29

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Neutrophils accelerate macrophage-mediated digestion of apoptotic cells in vivo as well as in vitro.2005

    • Author(s)
      Iyoda, T., Nagata, K., Akashi, M., Kobayashi, Y.
    • Journal Title

      J.Immunol. 175

      Pages: 3475-3483

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Inhibition by adiponectin of IL-8 production by human macrophages upon coculturing with late apoptotic cells.2005

    • Author(s)
      Saijo, S., Nagata, K., Nakano, Y., Tobe, T., Kobayashi, Y.
    • Journal Title

      Biochem Biophys Res Commun 334

      Pages: 1180-1183

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A novel accessory role of neutrophils in Concanavalin A-induced hepatitis.2005

    • Author(s)
      Hatada, S. et al.
    • Journal Title

      Cell.Immunol. 233

      Pages: 23-29

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Neutrophils accelerate macrophage-mediated digestion of apoptotic cells in vivo as well as in vitro.2005

    • Author(s)
      Iyoda, T. et al.
    • Journal Title

      J.Immunol. 175

      Pages: 3475-3483

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Inhibition by adiponectin of IL-8 production by human macrophages upon coculturing with late apoptotic cells.2005

    • Author(s)
      Saijo, S. et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 334

      Pages: 1180-1183

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Gene expression in the elicitation phase of guinea pig DTH and CHS reactions.2004

    • Author(s)
      Ohtani, M. et al.
    • Journal Title

      Cytokine 25

      Pages: 246-253

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Immature dendritic cells reduce proinflammatory cytokine production by a coculture of macrophages and apoptotic cells in a cell-cell contact-dependent manner.2004

    • Author(s)
      Takahashi M., et al.
    • Journal Title

      J. Leukoc. Biol. 75

      Pages: 865-873

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Gene expression in the elicitation phase of guinea pig DTH and CHS reactions.2004

    • Author(s)
      Ohtani, M., Kobayashi, Y., Watanabe, N.
    • Journal Title

      Cytokine 25

      Pages: 246-253

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Immature dendritic cells reduce proinflammatory cytokine production by a coculture of macrophages and apoptotic cells in a cell-cell contact-dependent manner.2004

    • Author(s)
      Takahashi M., Kurosaka K., Kobayashi, Y.
    • Journal Title

      J.Leukoc.Biol. 75

      Pages: 865-873

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Immature dendritic cells reduce proinflammatory cytokine production by a coculture of macrophages and apoptotic cells in a cell-cell contact-dependent manner.2004

    • Author(s)
      Takahashi M., et al.
    • Journal Title

      J.Leukoc.Biol. 75

      Pages: 865-873

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Involvement of MIP-2 and CXCR2 in neutrophil infiltration following injection of late apoptotic cells into the peritoneal cavity2004

    • Author(s)
      Iyoda T., Kobayashi Y
    • Journal Title

      Apoptosis 9

      Pages: 485-493

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Annexins I and IV inhibit Staphylococcus aureus attachment to human macrophages

    • Author(s)
      Gotoh M. et al.
    • Journal Title

      Immunology Letters (印刷中)

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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