| Co-Investigator(Kenkyū-buntansha) |
HIRAYAMA Teruhisa Kyoto Pharmaceutical University, School of Pharmacy, Professor, 薬学部, 教授 (10121568)
HASEI Tomohiro Kyoto Pharmaceutical University, School of Pharmacy, Research Assistant, 薬学部, 助手 (10388027)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Research Abstract |
Organic extracts from surface soil samples collected in Osaka and Aichi prefectures showed potent mutagenicity in S.typhimurium TA98 in the absence of S9 mix, inducing 1,240 - 233,000 revertants/mg of organic extract. Each soil extract was separated by chromatography using a Sephadex LH-20 column and a silica gel column. The fractions with potent mutagenicity were subsequently separated into five fractions, Fr. 1 - Fr. 5, using an Ultra pack ODS column. The contribution ratios of these five fractions to the total mutagenicity of each soil extract were similar ; Fr. 1 : 45% ; Fr. 2 : 15% ; Fr. 3 : 25% ; Fr. 4 : 5 ; Fr. 5 : 10%. These five fractions were further separated by HPLC. In consequence, it was found that mutagenic potencies of Fr. 1 and Fr. 3 were attributed to dinitropyrene (DNP) and 3,6-dinitrobenzo[e]pyrene (DNBeP), respectively. Major mutagens in Fr. 2, Fr. 4, and Fr. 5 were isolated from each soil extract, and these mutagens were expected to be nitroarenes on the basis of their mass spectra. Retention times of these mutagens in HPLC were not consistent with those of nitrosrenes, which had been detected in the environment. 3,6-DNBeP showed extremely potent mutagenicity in TA98 without S9 mix, inducing 285,000 revertants/nmol. This potency was comparable to that of 1,8-DNP, which is the strongest mutagen reported to date. Because 3,6-DNBeP showed higher mutagenicity in YG1024 than TA98, 3,6-DNBeP was thought to be activated by O-acetyltranferase for mutagenicity.
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