| Project/Area Number |
16590126
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Tokyo University of Pharmacy and Life Sciences |
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Principal Investigator |
KASUYA Yasuji Tokyo University of Pharmacy and Life Sciences, School of Pharmacy, Professor, 薬学部, 教授 (90096686)
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| Co-Investigator(Kenkyū-buntansha) |
FURUTA Takashi Tokyo University of Pharmacy and Life Sciences, School of Pharmacy, Associate Professor, 薬学部, 助教授 (70120152)
SHIBASAKI Hiromi Tokyo University of Pharmacy and Life Sciences, School of Pharmacy, Assistant Professor, 薬学部, 講師 (20206121)
YOKOKAWA Akitomo Tokyo University of Pharmacy and Life Sciences, School of Pharmacy, Assistant, 薬学部, 助手 (70328558)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | cortisol / cortisone / stable isotope methodology / 11β-activity / urinary cortisone / cortisol ratio / renal disease / hypertension / GC-MS-SIM / グリチルリチン酸 / 体内動態 / 速度論的解析 / 安定同位体標識 / 11β-HSD2酵素活性評価 |
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| Research Abstract |
This study is associated with a unique approach using stable isotope methodology in evaluating 11β-HSD2 activity in vivo based on urinary excretion of unconjugated (free) cortisol and cortisone. This stable isotope approach offered useful advantages since it allowed to measure stable isotopically labeled cortisol and its labeled metabolite cortisone the potential use of stable isotopically labeled cortisol in man as a tracer for the native cortisol to recognize the conversion of cortisol to cortisone in the kidney. Our results from the stable isotope methodology strongly suggest that the measurement of free cortisol and cortisone in urine is a significant advantage in assessing renal 1lb-HSD2 activity in man.
The kidney is a major site for the conversion of cortisol to cortisone. Since the urinary free cortisone/ cortisol ratio has been proved to be a specific in vivo measure of 11β-HSD2 activity in human kidney reflecting intra-renal concentrations of cortisol and cortisone, a further study was performed to recognize the conversion of cortisol to cortisone catalized by 11β-HSD2 in patients with renal disease.
This study provided direct evidence with regard to the usefulness of a urinary free cortisone/cortisol ratio as an index of human clinical tests for evaluating a reduced 11β-HSD2 activity.
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