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Mechanisms of gastrulation in newt embryo

Research Project

Project/Area Number 16590149
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKitasato University

Principal Investigator

OBATA Shuichi  Kitasato University, College of Liberal Arts and Sciences, Assis.Prof., 一般教育部, 講師 (10204273)

Co-Investigator(Kenkyū-buntansha) ONO Michio  Yokohama City University, Medicine, Assis.Prof., 医学部, 助手 (50264601)
SAWADA Hajime  Yokohama City University, Medicine, Prof., 大学院・医学研究科, 教授 (90101112)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordsearly development / morphogenesis / gastrulation / cell motility / cytoskeleton / 両生類胚 / アクチン / 微小管 / イモリ / 細胞接着 / チューブリン
Research Abstract

Gastrulation is one of important steps for morphogenesis in most species of multicellular animal embryos. The gastrulation is a complex phenomenon combined with several cellular events, for example, cell movements, cell shape changes, cell-cell and cell-matrix adhesions. Especially, the cell movements and cell shape changes are important to form an archenteron (primitive gut) and mesoderm layer. Holtfreter reported two types of unique cell movements, creeping movement of vermiform cells and circus movement characteristic of Amoeba, in isolated embryonic cells from amphibian gastrulae, and suggested that these cell movements were important in gastrulation of amphibian embryos. The creeping movement was suggested to play roles in drawing blastopore into the embryo. However, little is known about mechanisms of these cell movements and their roles playing in gastrulation yet. We investigated mechanisms of creeping movement to understand the physiological roles of this movement in gastrulat … More ion.
In early gastrula embryo, presumptive mesodermal cells invaginating into the embryo and a part of presumptive endodermal cells transformed into sausage-like shape. They elongated toward the direction of invagination of the archenteron. The elongated cells, called vermiform cells, were also observed both in the dissociated mesodermal and endodermal cells. A hyaline pseudopodium was formed at protruded end in the vermifom cell, and a small knob at the opposite end. We analyzed the creeping movement in the vermiform cells by time-lapse microscopy. The cells moved with the hyaline pseudopodium at the head. Several contracted sites were observed at the surface of the cell, simultaneously. The contraction waves at the cell surface moved backward, from the pseudopodium side to the knob side. Yolk granules and pigment granules also changed their positions inside the cells. By treatment of cytochalasin D (inhibitor for actin polymerization), the sausage-like shape of the vermiform cell quickly changed into round one. The elongation of the cells, contraction waves, and streaming of yolk granules and pigment granules were also completely inhibited by this treatment. Nocodazole (inhibitor for microtubule polymerization), however, did not affect them. Less

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (12 results)

All 2006 2005

All Journal Article (12 results)

  • [Journal Article] CyNodal, the Japanese newt nodal-related gene, is expressed in the left side of the lateral lmesoderm and diencephalon2006

    • Author(s)
      Yuzuru Ito
    • Journal Title

      Gene Expression Patterns 6

      Pages: 294-298

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] CyNodal, the Japanese newt nodal-related gene, is expressed in the left side of the lateral lmesoderm and diencephalons2006

    • Author(s)
      Yuzuru Ito
    • Journal Title

      Gene Expression Patterns 6

      Pages: 294-298

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] CyNodal, the Japanese newt nodal-related gene, i s expressed in the left side of the lateral mesoderm and diencephalon2006

    • Author(s)
      Yuzuru Ito
    • Journal Title

      Gene Expression Patterns 6

      Pages: 294-298

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 貧乏・ひまなし、だから始めるデジタルタイムラプス撮影入門 〜眠っている透過型光学顕微鏡を安価でデジタルタイムラプス実体顕微鏡として蘇らせる〜2005

    • Author(s)
      小畑秀一
    • Journal Title

      顕微鏡(Microscopy) 40(Suppl. 1)

      Pages: 93-98

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 蛍光ストロークだけじゃない! スペクトル共焦点レーザー顕微鏡による抗体クロストークの解決法2005

    • Author(s)
      尾野道男
    • Journal Title

      顕微鏡(Microscopy) 40(Suppl. 1)

      Pages: 87-91

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] TPAによる細胞の多核化メカニズムの解析2005

    • Author(s)
      吉田敬一郎
    • Journal Title

      顕微鏡(Microscopy) 40(Suppl. 1)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Only-it is not for fluorescent crosstalk2005

    • Author(s)
      Michio Ono
    • Journal Title

      Microscopy(in Japanese) 40 Suppl.1

      Pages: 87-91

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A trial for assembling inexpensive digital time-lapse microscope2005

    • Author(s)
      Shuichi Obata
    • Journal Title

      Microscopy(in Japanese) 40 Suppl.1

      Pages: 93-98

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The analysis of a multinucleation of the cell by the TPA stimulation2005

    • Author(s)
      Keiichiro Yoshida
    • Journal Title

      Microscopy(in Japanese) 40 Suppl.1

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 貧乏・ひま無し、だから始めるデジタルタイムラプス撮影入門〜眠っている透過型光学顕微鏡を安価でデジタルタイムラプス実体顕微鏡として蘇らせる〜2005

    • Author(s)
      小畑秀一
    • Journal Title

      顕微鏡(Microscopy) 40(Suppl.1)

      Pages: 93-98

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 蛍光ストロークだけじゃない!スペクトル共焦点レーザー顕微鏡による抗体クロストークの解決法2005

    • Author(s)
      尾野道男
    • Journal Title

      顕微鏡(Microscopy) 40(Suppl.1)

      Pages: 87-91

    • Related Report
      2005 Annual Research Report
  • [Journal Article] TPAによる細胞の多核化メカニズムの解析2005

    • Author(s)
      吉田敬一郎
    • Journal Title

      顕微鏡(Microscopy) 40(Suppl.1)

    • Related Report
      2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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