Study on the mechanisms of branching morphogenesis : toward the understanding of diversity of epithelia
| Project/Area Number |
16590151
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kitasato University |
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Principal Investigator |
KAODYA Yuichi Kitasato University, School of Medicine, Lecturer (10185887)
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| Project Period (FY) |
2004 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,180,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥180,000)
Fiscal Year 2007: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2006: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2005: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | basement membrane / salivary eland / branching morphogenesis / organ culture / cytoskeleton / laminin peptide / タイムラプス / 電子顕微鏡 / シグナル伝達 / 顎下腺 / 形態形成 / ラミニン / 増殖因子 / 微細構造 |
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| Research Abstract |
1. Ultrastructure of the clefts in the branching salivary gland epithelium
A laminin peptide, LVLFLNHGH (a functional peptide sequence located in the LG4 module of laminin-a5), was previously found to perturb in vitro branching morphogenesis of rudimental submandibular gland (SMG) of mice and results in the formation of many clefts in SMG. Here we found that typical clefts were 0.3mm width and deepened between adjacent cells. At the deepest end of more than 2/3 clefts, shut cytoplasmic projection, which is supported by actin filaments, was noted. These results suggest that the epithelial cleft formation is an active cell shape change driven by physical force that is generated by epithelial rolls.
2. Role of cytoskeleton on epithelial branching
The effects of cytochalasin D (CD) were tested for epithelial branching in the embryonic mice sublingual gland organ culture system. CD inhibited the branching at higher concentrations, however, at the lower concentration, CD stimulated the branching. These results indicated the involvement of actin filament dynamics for epithelial branching
3. Laminin peptides and cell morphology
The homologous peptides derived from the connecting loop regions between b-strands E and F in the laminin a chain LG4 modules (hEF-1-hEF-5) showed chain-specific cellular responses in various cell types. Among the peptides tested, hEF-1 and hEF-3 induced typical ruffling membrane and stress fibers in the fibroblasts, respectively.
4. Morphogenesis of pancreatic islets
We studied the organogenesis of islets in rat pancreas. During islet organogenesis, proliferation activity was high in exocrine duct system. Moreover, the endocrine duster enlarged by fusion of newly formed buds. We also found that the endocrine cell dusters maintained contact with the duct system throughout development. We concluded that the pancreatic islet is generated by the unification of multiple endocrine dusters originated from separate regions of the duct system.
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Report
(5 results)
Research Products
(60 results)
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[Journal Article] Identification of multiple amyloidogenic sequences in laminin-12007
Author(s)
Kasai, S., Urushibata, S., Hozumi, K., Yokoyama, F., Y., Ichikawa, N., I., Kadoya, Y., Nishi, N., Watanabe, N., Yamada, Y., Nomizu, M
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Journal Title
Biochemistry 46
Pages: 966-3974
Description
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[Journal Article] Integrin-dependent cell behavior on ECM peptide-conjugated chitosan membranes2007
Author(s)
Mochizuki, M., Yamagata, N., Philp, D., Hozumi, K., Watanabe, T., Kikkawa, Y., Kadoya, Y., Kleinman, HK., Nomizu, M
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Journal Title
Biopolymers 88
Pages: 122-130
Description
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[Journal Article] Laminin peptide-conjugated chitosan membrane : application for keratinocyte delivery in wounded skin2006
Author(s)
Ikemoto, S., Mochizuki, M., Yamada, M., Takeda, A., Uchinuma, E., Yamashina, S., Nomizu, M., Kadoya, Y
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Journal Title
J. Biomed. Mater. Res. A 79
Pages: 716-722
Description
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[Journal Article] Bifunctional peptides derived from homologous loop regions in the laminin a chain LG4 modules interact with both a 2 β 1 integrin and syndecan-22005
Author(s)
Yokoyama, F., Suzuki, N., Kadova, Y., Utani, A., Nakatsuka, H., Nishi, N., Haruki, M., Kleinman, H. K., Nomizu, M
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Journal Title
Biochemistry 44
Pages: 9581-9589
Description
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[Journal Article] Arikawa-Hirasawa E. : Identification of neurite outgrowth active sites on the laminin alpha4 chain G domain2005
Author(s)
Ichikawa, N., Kasai, S., Suzuki, N., Nishi, N., Oishi, S., Fujii, N., Kadoya, Y., Hatori, K., Mizuno, Y., Nomizu, M
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Journal Title
Biochemistry 44
Pages: 5755-5762
Description
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[Journal Article] Multifunctional peptide fibrils for biomedical materials2004
Author(s)
Kasai, S., Ohga, Y., Mochizuki, M., Nishi, N., Kadova, Y., Nomizu, M
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Journal Title
Biopolymers 76
Pages: 27-33
Description
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[Presentation] 上皮分岐部の微細構造2007
Author(s)
門谷 裕一
Organizer
第112回日本解剖学会総会、全国学術集会
Place of Presentation
大阪府大阪市
Description
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[Presentation] Laminin Peptide-Conjugated Chitosan Membrane : Application for Keratinocyte Delivery in Wounded Skin2006
Author(s)
Ikemoto, S., Masuda, R., Mochizuki, M., Yamada, M., Takeda, A., Uchinuma, E., Yamashina, S., Nomizu, M., Kadova, Y
Organizer
The 16th International Microscopy Congress
Place of Presentation
Sapporo, Japan
Description
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[Presentation] Laminin Peptide-Conjugated Chitosan Membrane : Application for Keratinocyte Delivery2006
Author(s)
Masuda, R., Mochizuki, M., Nomizu, M., Uchinuma, E., Yamashina, S., Kadoya, Y
Organizer
The 53th Annual Metting of the Japanese Matrix Club
Place of Presentation
Hakone, Japan
Description
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[Presentation] Effects of homologous regions of human laminin a chains for cell morphology and growth2006
Author(s)
Takahashi, N., Yokoyama, F., Kaodya, Y., Kikkawa, Y., Yamada, S., Nomizu, M
Organizer
The 53th Annual Metting of the Japanese Club
Place of Presentation
Hakone, Japan
Description
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[Presentation] Bifunctional biological peptides that act on integrin and syndecan2005
Author(s)
Yokoyama, F., Kadoya, Y., Suzuki, N., Utani, A., Haruki, M., Nomizu, M
Organizer
The 52th Annual Metting of the Japanese Club
Place of Presentation
Yufuin, Oita, Japan
Description
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[Presentation] Biological activities of cyclic peptide that mimics th eloop regions of laminin a3 chain LG4 module2005
Author(s)
Yokoyama, F., Kadoya, Y., Kato, K., Suzuki, N., Momota, Y., Utani, A., Haruki, M., Nomizu, M
Organizer
The 37th Annual Meeting of the Japanese Society for Connective Tissue Research
Place of Presentation
Toyama, Japan
Description
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[Presentation] Laminin active peptide-conjugated chitosan membranes as a new cell delivery system for wound dressing2004
Author(s)
Ikemoto, S., Mochizuki, M., Yamada, M., Nomizu, M., Takeda, A., Uchinuma, E., Yamashina, S., Kadova, Y
Organizer
Gordon Research Conference on Basement Membrane
Place of Presentation
Bristol, RI, USA
Year and Date
2004-06-14
Description
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[Presentation] Laminin peptide-conjugated chitosan membranes as a karatinocyte delivery system for wound dressing2004
Author(s)
Ikemoto, S., Mochizuki, M., Yamada, M., Nomizu, M., Takeda, A., Uchinuma, E., Yamashina, S., Kadova, Y
Organizer
The 36th Annual Meeting of the Japanese Society for Connective Tissue Research
Place of Presentation
Fukuoka, Japan
Description
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[Presentation] Role of laminin-a5 chain LG4 module in epithelial branching morphogenesis2004
Author(s)
Kadoya, Y., Mochizuki, M., Nomizu, M., Sorokin, L., Yamashina, S
Organizer
The 16th International Congress of the IFAA and 109th Annual Meeting
Place of Presentation
Kyoto, Japan
Description
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[Presentation] AG-73 peptide conjugated chitosan membrane2004
Author(s)
Ikemoto, S., Mochizuki, M., Yamada, M., Nomizu, M., Takeda, A., Uchinuma, E., Yamashina, S., Kadoya, Y
Organizer
The 47th Meeting of Japan Society of Plastic and Reconstruction Surgery
Place of Presentation
Shinjuku, Tokyo, Japan
Description
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