| Project/Area Number |
16590152
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kyorin University |
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Principal Investigator |
KANAI Masami Kyorin University, Medical School, Lecture, 医学部, 講師 (70321883)
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| Co-Investigator(Kenkyū-buntansha) |
YONEKAWA Hiromichi Tokyo metropolitan institute of medical science, Vice director, 東京都臨床医学総合研究所, 副所長 (30142110)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | endoderm / mouse / development / gut formation / Sox17因子 |
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| Research Abstract |
The Sox (Sry-related HMG box) gene family was first identified through its homology to the high mobility group (HMG) box of the sex determining factor gene SRY. Members of the Sox family have been isolated in various vertebrate species, including 20 Sox genes in humans and mice. Members of this gene family encode transcription factors that regulate the specification of cell types and tissue differentiation in various developmental processes. Analyses of Sox17 expression have revealed activity in the visceral and definitive endoderm of post-implantation mouse embryos. In this project, we have studied that the role of Sox17 on the mouse endoderm derived gut formation. mRNA of Sox17 is expressed on definitive entire gut endoderm at first, and may function as a maintenance factor in prospective foregut, and differenaitation regulator at mid and hindgut. The later stage, mRNA of Sox17 is expressed on hepatic diverticum. The liver from the heterozygous embryo also shows the alteration of peripheral region of lobes. The major phenotype observed is the decrease of approximate weight of the liver. This is a preliminary data, but the effect of genetical backrgound to the hepato-insufficisncycy are necessary to be observed in the further study. Our observation strongly indicate that a critical role of Sox17 in the determination of the cell into the endoderm and its derivative organ in mammals. These findings lead us to the possibility that Sox17 is one of the key genes for the isolation of the endodermal stem cells and obtaining of the endodermal cells derived from ES cells in the human therapeutic research for the future.
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