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Genetic analysis of excitatory α_1-adrenergic receptor in insulin secreting islet β-cells of the rat

Research Project

Project/Area Number 16590161
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionHirosaki University

Principal Investigator

SUGA Sechiko  Hirosaki University, Center for Education and Research of Lifelong Learning, Associate Professor, 生涯学習教育研究センター, 助教授 (80003408)

Co-Investigator(Kenkyū-buntansha) WAKUI Makoto  Hirosaki University, School of Medicine, Professor, 医学部, 教授 (80108505)
KIMURA Ryoichi  Hirosaki University, School of Medicine, Assistant, 医学部, 助手 (20343022)
ITOI Keiichi  Tohoku University, Graduate School of Information Sciences, Professor, 大学院・情報科学研究科, 教授 (60232427)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsIslet b-cells / α_1-adrenoceptor / Receptor antibody / PCR / Hepatocyte / Insulin secretion / Phospholipase C / α_2-アドレナリン受容体 / 百日咳毒素 / L-型Ca^<2+>チャネル / 電位依存性K^+チャネル
Research Abstract

In the insulin secretion of islet β-cells, the excitatory effect of norepinephrine (NE) linking to activation of phospholipase C (PLC) is known to be present, although that effect is masked by the inhibitory effect of NE through the pertussis toxin (PTX)-sensitive pathway. The present study was carried out to examined whether α_1-adrenoceptors were present in islet p-cells, and if present, what subtype of α_1-radrenoceptor was responsible for the excitatory action of NE.
In the PCR analysis for islets, three bands of 212 bp,300 bp and 304 bp, corresponding α_<1A>-,α_<1B>- and α_<1D>-adrenoceptors, were obtained. Compared with 212 and 300 bands, 304 band was weak. These results indicate that in islets, there are three subtypes of α_1-adrenoceptors, i.e.,α_<1A>,α_<1B> and α_<1D>, and that α_<1A> and α_<1B> are more prominent than α_<1D>. The PCR analysis also showed the presence of such α_1-adrenoceptor subtypes in rat liver tissues. In order to explore the localization of these receptor subtypes, we examined immunofluorescence using receptor antibodies. In the islets of slice preparations of rat pancreas, none of α_<1A>-,α_<1B>- or α_<1D>-adrenoceptors were seen. In addition, these receptor subtypes were also not observed in the area out of the islets. Furthermore, from the immunofluorescence study on liver slices, these three subtypes of α_1-adrenoceptors were not observed. These results indicate that although the messages for α_1-adrenoceptor subtypes (α_<1A>,α_<1B>,α_<1D>) are present in rat islets, their locarization is not confirmed because of the use of inadequate antibodies for the receptors.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (7 results)

All 2005 2004

All Journal Article (7 results)

  • [Journal Article] Iptakalim hydrochloride and neuronal protection.2005

    • Author(s)
      Jie Wu
    • Journal Title

      Current Neuropharmacology 3

      Pages: 249-256

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Role of α7-nicotinic acetylcholine receptors in tetanic stimulation-induced γ oscillations in rat hippocampal slices.2005

    • Author(s)
      Jie Wu
    • Journal Title

      Neuropharmacology 48

      Pages: 869-880

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Staphylococcal enterotoxin A modulates intracellular Ca^<2+> signal pathway in human intestinal epithelial cells.2005

    • Author(s)
      Dong-Liang Hu
    • Journal Title

      FEBS Letters 579

      Pages: 4407-4412

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Role of α7-nicotinic acetylcholine receptors in titanic stimulation-induced γossilations in rat hippocampal slices.2005

    • Author(s)
      Chuanzhe Song
    • Journal Title

      Neuropharmacology 48

      Pages: 869-880

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Staphylococcal enterotoxin A modulates intracellular Ca^<2+> signal pathway in human intesitinal epithelial cells.2005

    • Author(s)
      Dong-Liang Hu
    • Journal Title

      FEBS Letters 579

      Pages: 4407-4412

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 2-Aminoethoxydiphenyl borate inhibits agonist-induced Ca2+ signals by blocking inositol trisphosphate formation in acutely dissociated mouse pancreatic acinar cells.2004

    • Author(s)
      Jie Wu
    • Journal Title

      Pflugers Archiv (Eur.J.Physiol.) 448

      Pages: 592-595

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Pertussis toxin-sensitive pathway inhibits glucose-stimulated Ca2+ signals of rat islet beta-cells by affecting L-type Ca2+ channels and voltage-dependent K+ channels.2004

    • Author(s)
      Sechiko Suga
    • Journal Title

      Cell Calcium 36

      Pages: 469-477

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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