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Optical imaging analysis of the functional organization of the receptor-intracellular signal transduction system in the early embryonic heart.

Research Project

Project/Area Number 16590166
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionUniversity of the Ryukyus

Principal Investigator

SAKAI Tetsuro  University of the Ryukyus, Faculty of Medicine, Professor, 医学部, 教授 (40153845)

Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsOptical recording / Voltage-sensitive dye / Automatism / Receptor / Intracellular signal transduction / Functional organization / Imaging
Research Abstract

Voltage-sensitive dye-related absorption signals have provided a new method for monitoring spontaneous electrical activity in the early embryonic heart. We have introduced multiple-site optical recording of transmembrane voltage activity, using a fast merocyanine-rhodanine voltage-sensitive dye (NK2761) and a multi-element (16 X 16) photodiode array, to monitor action potentials in the early embryonic chick hearts at the stages when the first muscle contractions appear. By the application of adrenaline, the rhythm of the cardiac pacemaker became faster although the effect of this drug was very weak in comparison with that in adult hearts. On the other hand, the application of caffeine accelerated the rhythm of the pacemaker, suggesting that the intracellular signal transduction system was partially functioning. However, the application of adrenaline slowed the conduction velocity of the excitatory wave from the pacemaker. These results suggest that, in the early embryonic heart, the intracellular signal transduction system is somewhat different from that of adult hearts.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (19 results)

All 2006 2005 2004

All Journal Article (19 results)

  • [Journal Article] Cytochalasin D as the Depressant of Contraction for the Optical Monitoring of Action Potentials in Isolated Rat Atrium2006

    • Author(s)
      SAKAI, T
    • Journal Title

      Journal of Physiological Sciences 56・5

      Pages: 385-388

    • NAID

      10019380238

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Use of Cytochalasin D as the Uncoupler of E-C Coupling for the Optical Monitoring of Action Potentials in the Isolated Rat Atrium.2006

    • Author(s)
      SAKAI, T
    • Journal Title

      Journal of Physiological Sciences 56・Suppl

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cytochalasin D as the Depressant of Contraction for the Optical Monitoring of Action Potentials in Isolated Rat Atrium2006

    • Author(s)
      Sakai T
    • Journal Title

      Journal of Physiological Sciences 56

      Pages: 385-388

    • NAID

      10019380238

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Use of Cytochalasin D as the Uncoupler of E-C Coupling for the Optical Monitoring of Action Potentials in the Isolated Rat Atrium2006

    • Author(s)
      Sakai T
    • Journal Title

      Journal of Physiological Sciences 56Suppl

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Use of cytochalasin D as the uncoupler of EC coupling for the optica monitoring of action potentials in the isolatcd rat atrium2006

    • Author(s)
      SAKAI, T
    • Journal Title

      Journal of Physiological Sciences 56・Suppl

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A regional gradient of intrinsic rhythmicity depicted in embryoni cultured muliple-hearts.2006

    • Author(s)
      SAKAI, T
    • Journal Title

      Seibutsu Butsuri 46・Supp12

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Optical Mapping of Tachycardia-like Excitation Evoked by Pacing in the Isolated Rat Atrium2005

    • Author(s)
      SAKAI, T
    • Journal Title

      Japanese Journal of Physiology 54・6

      Pages: 593-599

    • NAID

      130007038473

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Wavelength Dependence of Optical Action Potentials in the Isolated Rat Atrium2005

    • Author(s)
      SAKAI, T
    • Journal Title

      Japanese Journal of Physiology 55・6

      Pages: 389-393

    • NAID

      10018087395

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Action Spectra of Voltage -sensitive Dyes in the Isolated Rat Atrium2005

    • Author(s)
      SAKAI, T
    • Journal Title

      Japanese Journal of Physiology 55・Suppl.

    • NAID

      130005448013

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Wavelength Dependence of Optical Action Potentials in the Isolated Rat Atrium2005

    • Author(s)
      Sakai, T
    • Journal Title

      Japanese Journal of Physiology 55

      Pages: 389-393

    • NAID

      10018087395

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Action Spectra of Voltage-sensitive Dyes in the Isolated Rat Atrium2005

    • Author(s)
      Sakai, T
    • Journal Title

      Japanese Journal of Physiology 55Suppl

    • NAID

      130005448013

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Wavelength Dependence of Optical Action Potentials in the Isolated Rat Atrium2005

    • Author(s)
      Sakai, T
    • Journal Title

      Japanese Journal of Physiology 55・6

      Pages: 389-393

    • NAID

      10018087395

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Action Spectra of Voltage-sensitive Dyes in the Isolated Rat Atrium2005

    • Author(s)
      Sakai, T
    • Journal Title

      Japanese Journal of Physiology 55・Suppl.

    • NAID

      130005448013

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Optical mapping of tachycardia-like excitation evoked by pacing in the isolated rat atrium2005

    • Author(s)
      Sakai, T.
    • Journal Title

      Japanese Journal of Physiology 54・6

      Pages: 593-599

    • NAID

      130007038473

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Optical Mapping of Tachycardia-like Excitation Evoked by Pacing in the Isolated Rat Atrium2004

    • Author(s)
      SAKAI, T
    • Journal Title

      Japanese Journal of Physiology 54・Suppl

    • NAID

      130007038473

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Optical Mapping of Tachycardia-like Excitation Evoked by Pacing in the Isolated Rat Atrium2004

    • Author(s)
      Sakai, T
    • Journal Title

      Japanese Journal of Physiology 54

      Pages: 593-599

    • NAID

      130007038473

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Optical Mapping of Tachycardia-like Excitation Evoked by Pacing in the Isolated Rat Atrium2004

    • Author(s)
      Sakai, T
    • Journal Title

      Japanese Journal of Physiology 54Suppl

    • NAID

      130007038473

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Optical mapping of tachycardia-like excitation evoked by pacing in the isolated rat atrium2004

    • Author(s)
      Sakai, T.
    • Journal Title

      Japanese Journal of Physiology 54 Suppl.

    • NAID

      130007038473

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 心臓興奮伝播パターンの光学的マッピング(review)2004

    • Author(s)
      酒井哲郎
    • Journal Title

      琉球医学会誌 23・3

      Pages: 59-69

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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