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Two-oscillator coupling in circadian clock

Research Project

Project/Area Number 16590173
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionUniversity of Fukui (2005)
Hokkaido University (2004)

Principal Investigator

ABE Hiroshi  University of Fukui, Faculty of Medical Sciences, Professor, 医学部, 教授 (80201896)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsCircadian rhythm / Biological clock / Suprachiasmatic nucleus / Behavior / Clock gene / CS mouse / Rhythm splitting / Methamphetamine / リズムスプリッティング / 制限給餌サイクル
Research Abstract

Two-oscillator model of circadian clock has been suggested to be 1) Evening (E) and Morning (M) oscillators, 2) master pacemaker in the suprachiasmatic nucleus (SCN) and food-entrainable oscillator (FEO) under restricted feeding schedule (RF), and 3) the SCN pacemaker and methamphetamine (MAP)-induced oscillator. Present study examined the functional relationship between E/M oscillators, FEO, and MAP oscillator by using CS mouse which shows 'splitting' in the circadian rhythm of behavior and obtained following results.
1. To establish the stable condition which induces 'splitting' in behavioral rhythm of CS mouse, three modified light-dark conditions (LD6:6:6:6 h, LD9:6:3:6 h and LD9:15 h) were tested for the mouse housed in a running-wheel cage. However, CS mice did not show splitting in the running-wheel activity rhythm under these conditions. This indicates that constant darkness (DD) is the best condition to induce 'splitting' in the circadian rhythm of CS mouse.
2. Under RF (3 h feeding) in DD, behavioral rhythm of CS mouse was entrained by RF. In addition, clock gene (mPer1, mPer2 and mBMAL1) expression rhythms in the SCN were entrained by RF. These results indicate that the SCN pacemaker of CS mouse is entrained by RF unlike that of rats and other strain of mouse (C57BL/6J). During RF and the ad lib feeding after RF, behavioral rhythm did not show 'splitting', suggesting that the E/M oscillator might be associated with FEO in the CS mouse.
3. Administration of 0.01% MAP in drinking water under DD induced the activity component which freeran with lengthened period in addition to the freerunning component of split rhythm. After MAP administration, M component of split rhythm disappeared. These results suggest that there is no relationship between E/M oscillators and MAP oscillator, and that the M oscillator might be influenced by MAP administration.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (2 results)

All 2004

All Journal Article (2 results)

  • [Journal Article] Circadian rhythms in behavior and clock gene expressions in the brain of mice lacking histidine decarboxylase.2004

    • Author(s)
      Abe, H.
    • Journal Title

      Molecular Brain Research 124

      Pages: 178-187

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 時間遺伝子によるリズム発振:主時計と末梢時計の脱同調2004

    • Author(s)
      安倍 博
    • Journal Title

      臨床脳波 46卷4号

      Pages: 229-236

    • Related Report
      2004 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

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