Molecularpharmacological analysis of mechanosensitive cation channels in vascular endothelial cells.
| Project/Area Number |
16590189
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Akita University |
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Principal Investigator |
IIJIMA Toshihiko Akita University, School of Medicine, Professor, 医学部, 教授 (30004724)
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| Co-Investigator(Kenkyū-buntansha) |
MURAKAMI Manabu Akita University, School of Medicine, Lecturer, 医学部, 講師 (80302090)
SATOH Eisaku Aomori University, Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (10282162)
ONO Kyouichi Akita University, School of Medicine, Associate Professor, 医学部, 助教授 (70185635)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | TRP / smooth muscle / calcium / channel / tachycardia / 平滑筋 / カチオンチャネル / マウス / 遺伝子 |
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| Research Abstract |
1)Identification of murine PKD2L1 (TRPP2L1)
We cloned murine PKD2L1 gene, which is a member of TRP channel family. PKD2L1 forms functional cation channels on the plasma membrane by interacting with PKD1 (Murakami et al., J.Biol.Chem., 2005).
2)Functional analysis of TRPC
Using overexpression transgenic mouse, regulatory role of neuron-restrictive silencing factor (NRSF) in expression of TRPC1 was clarified (Ohba et al., 2005).
Regulatory effect of unoprostone on store operated Ca^<2+> entry (SOCE) in human aortic smooth muscle cells was investigated (Watanabe et al., 2005).
3)Functions of voltage-dependent calcium channels
As a cation channel family member, we investigated functions of voltage-dependent calcium channels.
Roles of voltage- dependent calcium channels in nociceptive neurotransmission were analyzed (Murakami et al., Neuroscience letters, 2004, Murakami et al., Brain Res., 2004). Importance of β3 subunit on insulin secretion through increase in IP3 production was proved (Berggren, et al., 2004). Inhibitory effects of perfuorooctane sulfonate (PFOS) and perfuorooctanoate (PFOA) on L-type calcium channels were evaluated (Harada et al., 2005). We clarified involvement of Thromboxane A2 and prostaglandin F2α in inflammatory tachycardia (Takayama et al., 2005).
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Report
(3 results)
Research Products
(24 results)
