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Identification of new Na^<+->driven cation transporter and its functional and pathophysiological analyses

Research Project

Project/Area Number 16590213
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General pharmacology
Research InstitutionFukuoka University

Principal Investigator

IWAMOTO Takahiro  Fukuoka University, School of Medicine, Lecturer, 医学部, 講師 (20300973)

Co-Investigator(Kenkyū-buntansha) MIGITA Keisuke  Fukuoka University, School of Medicine, Assistant, 医学部, 助手 (10352262)
ARAI Yuji  National Cardiovascular Center Research Institute, Department of Bioscience, Laboratory Chief, バイオサイエンス部, 室長 (30202724)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsNa^+ / Ca^<2+> exchanger / Cation transporter / Expression system / Transgenic mice / Tissue distribution / イオントランスポーター / 薬理学 / 生理学
Research Abstract

Abnormal intracellular Na^+ metabolism is related to several diseases, such as hypertension, arteriosclerosis, ischemic injury, renal failure, and diabetes. Recently, we found that the role of vascular Na^+/Ca^<2+> exchanger in the pathogenesis of salt-dependent hypertension using specific exchange inhibitors and genetically engineered mice. In this study, we searched a new Na^<+->driven cation transporter, which may be involved in Na^+-dependent diseases, and identified the new transporter by gene analyses using specific motif sequences (α-repeat) of Na^+/Ca^<2+> exchanger superfamily. The identified gene was partially homologous to K^+-dependent Na^+/Ca^<2+> exchanger (NCKX). This new NCKX was abundantly expressed in heart, smooth muscle, and skeletal muscle. When this new NCKX cDNA was expressed in mammalian cells, we could detect Na^+-dependent Ca^<2+> transport activity. We are currently preparing genetically engineered mice for this new NCKX. In near future, we would like to examine the functional and pathophysiological importance of this new Nat^+-driven cation transporter using genetically engineered mice.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (53 results)

All 2006 2005 2004

All Journal Article (53 results)

  • [Journal Article] Na^+/Ca^<2+>交換体(NCX1)と食塩感受性高血圧2006

    • Author(s)
      岩本隆宏
    • Journal Title

      日本臨床 64

      Pages: 167-176

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Vascular Na^+/Ca^<2+> exchanger : implications for the pathogenesis and therapy of salt-dependent hypertension.2006

    • Author(s)
      Iwamoto, T.
    • Journal Title

      Am. J. Physiol. 290

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 食塩感受性高血圧とNa^+/Ca^<2+>交換体 : 食塩負荷から血管トーヌス亢進への古くて新しい機序2006

    • Author(s)
      岩本隆宏
    • Journal Title

      日薬理誌 127

      Pages: 387-392

    • NAID

      10018061856

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Hypertension, Na^+/Ca^<2+> exchanger, and Na^+,K^+-ATPase.2006

    • Author(s)
      Iwamoto, T.
    • Journal Title

      Kidney Inter. (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Vascular Na^+/Ca^<2+> exchanger : implications for the pathogenesis and therapy of salt-dependent hypertension.2006

    • Author(s)
      Iwamoto, T.
    • Journal Title

      Am.J.Physiol. 290

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Vascular Na^+/Ca^<2+> exchanger: implications for the pathogenesis and therapy of salt-dependent hypertension.2006

    • Author(s)
      Iwamoto, T.
    • Journal Title

      Am. J. Physiol 290・3

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Na^+/Ca^<2+>交換体(NCXl)と食塩感受性高血圧2006

    • Author(s)
      岩本隆宏
    • Journal Title

      日本臨床 64・1

      Pages: 167-176

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Different cation sensitivities and binding site domains of Na^+-Ca^<2+>-k^+ and Na^+-Ca^<2+> exchangers.2005

    • Author(s)
      Uehara, A.
    • Journal Title

      J. Cell Physiol. 203

      Pages: 420-428

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Sodium-calcium exchange inhibitors : therapeutic potential in cardiovas cular diseases.2005

    • Author(s)
      Iwamoto, T.
    • Journal Title

      Future Cardiol. 1

      Pages: 519-529

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Salt-sensitive hypertension, Na^+/Ca^<2+> exchanger, and vascular smooth muscle.2005

    • Author(s)
      Iwamoto, T.
    • Journal Title

      Trends Cardiovasc. Med. 15

      Pages: 273-277

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Adenosine induces ATP release via an inositol 1,4,5-trisphosphate signaling pathway in MDCK cells.2005

    • Author(s)
      Migita, K.
    • Journal Title

      Biochem. Biophys. Res. Commun. 328

      Pages: 1211-1215

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Down-regulation of Na^+/Ca^<2+> exchanger by fluvastatin in rat cardiomyoblast H9c2 cells : involvement of RhoB in Na^+/Ca^<2+> exchanger mRNA stability.2005

    • Author(s)
      Maeda, S.
    • Journal Title

      Mol. Pharmacol. 68

      Pages: 414-420

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Sodium pump α2 subunits control myogenic tone and blood pressure.2005

    • Author(s)
      Zhang J.
    • Journal Title

      J. Physiol. 569

      Pages: 243-256

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Functional differences of Na^+/Ca^<2+> exchanger expression in Ca^<2+> trans port system of smooth muscle of guinea pig stomach.2005

    • Author(s)
      Sakal, Y.
    • Journal Title

      Can. J. Physiol. Pharmacol. 83

      Pages: 791-797

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Different cation sensitivities and binding site domains of Na^+-Ca^<2+->K^+ and Na^+-Ca^<2+> exchangers.2005

    • Author(s)
      Uehara, A.
    • Journal Title

      J.Cell Physiol. 203(2)

      Pages: 420-428

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Sodium-calcium exchange inhibitors : therapeutic potential in cardiovascular diseases.2005

    • Author(s)
      Iwamoto, T.
    • Journal Title

      Future Cardiol. 1(4)

      Pages: 519-529

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Salt-sensitive hypertension, Na^+/Ca^<2+> exchanger, and vascular smooth muscle.2005

    • Author(s)
      Iwamoto, T.
    • Journal Title

      Trends Cardiovasc.Med. 15(8)

      Pages: 273-277

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Adenosine induces ATP release via an inositol 1,4,5-trisphosphate signaling pathway in MDCK cells.2005

    • Author(s)
      Migita, K.
    • Journal Title

      Biochem.Biophys.Res.Commun. 328(4)

      Pages: 1211-1215

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Down-regulation of Na^+/Ca^<2+> exchanger by fluvastatin in rat cardiomyoblast H9c2 cells : involvement of RhoB in Na^+/Ca^<2+> exchanger mRNA stability.2005

    • Author(s)
      Maeda, S.
    • Journal Title

      Mol.Pharmacol. 68(2)

      Pages: 414-420

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Sodium pump α2 subunits control myogenic tone and blood pressure.2005

    • Author(s)
      Zhang J.
    • Journal Title

      J.Physiol. 569(1)

      Pages: 243-256

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Functional differences of Na^+/Ca^<2+> exchanger expression in Ca^<2+> transport system of smooth muscle of guinea pig stomach.2005

    • Author(s)
      Sakai, Y.
    • Journal Title

      Can.J.Physiol.Pharmacol. 83(8-9)

      Pages: 791-797

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Adenosine induces ATP release via an inositol 1, 4, 5-trisphosphate signaling pathway in MDCK cells.2005

    • Author(s)
      Migita, T.
    • Journal Title

      Biochem. Biophys. Res. Commun 328・4

      Pages: 420-428

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Down-regulation of Na^+/Ca^<2+> exchanger by fluvastatin in rat cardiomyoblast H9c2 cells: involvement of RhoB in Na^+/Ca^<2+> exchanger mRNA stability.2005

    • Author(s)
      Maeda, T.
    • Journal Title

      Mol. Pharmacol 68・2

      Pages: 414-420

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Sodium pump α2 subunits control myogenic tone and blood pressure.2005

    • Author(s)
      Zhang, J.
    • Journal Title

      J. Physiol 569・1

      Pages: 243-256

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Functional differences of Na^+/Ca^<2+> exchanger expression in Ca^<2+> transport system of smooth muscle of guinea pig stomach.2005

    • Author(s)
      Sakai, Y.
    • Journal Title

      Can. J. Physiol. Pharmacol 83・8-9

      Pages: 791-797

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Salt-sensitive hypertension, Na^+/Ca^<2+> exchanger, and vascular smooth muscle.2005

    • Author(s)
      Iwamoto, T.
    • Journal Title

      Trends Cardiovasc. Med 15・8

      Pages: 273-277

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Sodium-calcium exchange inhibitors: therapeutic potential in cardiovascular diseases.2005

    • Author(s)
      Iwamoto, T.
    • Journal Title

      Future Cardiol 1・4

      Pages: 519-529

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Different cation sensitivities and binding site domains of Na^+-Ca^<2+>-K^+ and Na^+-Ca^<2+> exchangers.2005

    • Author(s)
      Uehara, A.
    • Journal Title

      J. Cell Physiol. 203・2

      Pages: 420-428

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Development and application of Na^+/Ca^<2+> exchange inhibitors.2004

    • Author(s)
      Iwaimoto, T.
    • Journal Title

      Mol. Cell Biochem. 259

      Pages: 157-161

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular determinants of Na^+/Ca^<2+> exchange (NCX1) inhibition by SEA0400.2004

    • Author(s)
      Iwamoto, T.
    • Journal Title

      J. Biol. Chem. 279

      Pages: 7544-7553

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The exchanger inhibitory peptide region-dependent inhibition of Na^+/Ca^<2+> exchange by SN-6 [2-[4-(4-nitrobenzyloxy)benzyl]thiazolidine-4-carboxylic acid ethyl ester], a novel benzyloxyphenyl derivative.2004

    • Author(s)
      Iwamoto, T.
    • Journal Title

      Mol. Pharmacol. 66

      Pages: 44-55

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of amiodarone on mutant Na^+/Ca^<2+> exchangers expressed in CCL39 cells.2004

    • Author(s)
      Watanabe, Y.
    • Journal Title

      Eur. J. Pharmacol. 496

      Pages: 49-54

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Salt-sensitive hypertension is triggered by Ca^<2+> entry via Na^+/Ca^<2+> exchanger type-1 in vascular smooth muscle.2004

    • Author(s)
      Iwamoto, T.
    • Journal Title

      Nature Med. 10

      Pages: 1193-1199

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Endothelin-1 enhances the activity of Na^+/Ca^<2+> exchanger type 1 in renal epithelial cells.2004

    • Author(s)
      Kita, S.
    • Journal Title

      J. Cardiovase. Pharmacol. 44

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Endotihelin-1 aggravates hypoxia/reoxygenation-induced injury in renal epithelial cells through the activation of Na^+/Ca^<2+> exchanger.2004

    • Author(s)
      Iwamoto, T.
    • Journal Title

      J. Cardiovasc. Pharmacol. 44

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Na^+/Ca^<2+>交換体と循環器疾患2004

    • Author(s)
      岩本 隆宏
    • Journal Title

      臨床化学 33

      Pages: 105-112

    • NAID

      130005447881

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 新規Na^+/Ca^<2+>交換輸送体阻害薬SN-6の作用特性および腎尿細管保護作用2004

    • Author(s)
      井上 優
    • Journal Title

      CLINICAL CALCIUM 14

      Pages: 1262-1269

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Forefront of Na^+/Ca^<2+> exchanger studies : Molecular Pharmacology of Na^+/Ca^<2+> exchange inhibitors.2004

    • Author(s)
      Iwamoto, T.
    • Journal Title

      J. Pliarmacol. Sci. 96

      Pages: 27-32

    • NAID

      10014166162

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Forefront of Na^+/Ca^<2+> exchanger studies : Physiology and molecular biology of monovalent cation sensitivities in Na^+/Ca^<2+> exchangers.2004

    • Author(s)
      Uehara, A.
    • Journal Title

      J. Pharmacol. Sci. 96

      Pages: 19-22

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Development and application of Na^+/Ca^<2+> exchange inhibitors.2004

    • Author(s)
      Iwamoto, T.
    • Journal Title

      Mol.Cell Biochem. 259(1-2)

      Pages: 157-161

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular determinants of Na^+/Ca^<2+> exchange (NCX1) inhibition by SEA0400.2004

    • Author(s)
      Iwamoto, T.
    • Journal Title

      J.Biol.Chem. 279(9)

      Pages: 7544-7553

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The exchanger inhibitory peptide region-dependent inhibition of Na^+/Ca^<2+> exchange by SN-6[2-[4-(4-nitrobenzyloxy)-benzyl]thiazolidine-4-carboxylic acid ethyl ester], a novel benzyloxy-phenyl derivative.2004

    • Author(s)
      Iwamoto, T.
    • Journal Title

      Mol.Pharmacol. 66(1)

      Pages: 45-55

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of amiodarone on mutant Na^+/Ca^<2+> exchangers expressed in CCL39 cells.2004

    • Author(s)
      Watanabe, Y.
    • Journal Title

      Eur.J.Pharmacol. 496(1-3)

      Pages: 49-54

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Salt-sensitive hypertension is triggered by Ca^<2+> entry via Na^+/Ca^<2+> exchanger type-1 in vascular smooth muscle.2004

    • Author(s)
      Iwamoto, T.
    • Journal Title

      Nature Med. 10(11)

      Pages: 1193-1199

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Endothelin-1 enhances the activity of Na^+/Ca^<2+> exchanger type 1 in renal epithelial cells.2004

    • Author(s)
      Kita, S.
    • Journal Title

      J.Cardiovasc.Pharmacol. 44(Suppl 1)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Endothelin-1 aggravates hypoxia/reoxygenation-induced injury in renal epithelial cells through the activation of Na^+/Ca^<2+> exchanger.2004

    • Author(s)
      Iwamoto, T.
    • Journal Title

      J.Cardiovasc.Pharmacol. 44(Suppl 1)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Forefront of Na^+/Ca^<2+> exchanger studies : Molecular Pharmacology of Na^+/Ca^<2+> exchange inhibitors.2004

    • Author(s)
      Iwamoto, T.
    • Journal Title

      J.Pharmacol.Sci. 96(1)

      Pages: 27-32

    • NAID

      10014166162

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Forefront of Na^+/Ca^<2+> exchanger studies : Physiology and molecular biology of monovalent cation sensitivities in Na^+/Ca^<2+> exchangers.2004

    • Author(s)
      Uehara, A.
    • Journal Title

      J.Pharmacol.Sci. 96(1)

      Pages: 19-22

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The exchanger inhibitory peptide region-dependent inhibition of Na^+/Ca^<2+> exchange by SN-6 [2-[4-(4-nitrobenzyloxy)benzyl]thiazolidine-4-carboxylic acid ethyl ester], a novel benzyloxyphenyl derivative.2004

    • Author(s)
      Iwamoto, T.
    • Journal Title

      Mol.Pharmacol. 66・1

      Pages: 44-55

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Salt-sensitive hypertension is triggered by Ca^<2+> entry via Na^+/Ca^<2+> exchanger type-1 in vascular smooth muscle2004

    • Author(s)
      Iwamoto, T.
    • Journal Title

      Nature Med. 10・11

      Pages: 1193-1199

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of amiodarone on mutant Na^+/Ca^<2+> exchangers expressed in CCL39 cells.2004

    • Author(s)
      Watanabe, Y.
    • Journal Title

      Eur J Pharmacol. 296・1-3

      Pages: 49-54

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Endothelin-1 aggravates hypoxia/reoxygenation-induced injury in renal epithelial cells through the activation of a Na^+/Ca^<2+> exchanger.2004

    • Author(s)
      Iwamoto, T.
    • Journal Title

      J.Cardiovasc.Pharmacol. 44・S1

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Endotheline-1 enhances the activity of Na^+/Ca^<2+> exchanger type 1 in renal epithelial cells.2004

    • Author(s)
      Kita, S.
    • Journal Title

      J.Cardiovasc.Pharmacol. 44・S1

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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