Regulation of the Class Switch Recombination
| Project/Area Number |
16590226
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General medical chemistry
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
SUGAI Manabu Kyoto University, Graduate School of Medicine, Associate Professor, 医学研究科, 助手 (90303891)
|
|---|
| Project Period (FY) |
2004 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
|
|---|
| Keywords | E2A / Pax5 / Id2 / Bcl6 / NFkB / IRF4 / STAT6 / NFkB / AID / STAT6 / IgE / CpG |
|---|
| Research Abstract |
The CpG motif in DNA plays a critical role in immunity via modulating the Th1/Th2 balance. In B cells, CpG-containing oligodeoxynucleotides (CpG ODNs) inhibit IL-4-mediated class switch recombination (CSR) to IgG1 and IgE through inhibition of the germline transcription (GLT) of these isotypes. However, the molecular mechanism of this inhibitory effect remains elusive. We showed that Id2 and Bcl6, both of which inhibit IgE GLT and CSR, are not involved in this inhibitory pathway. We demonstrated that there is reduced activity of NFκB binding to the IgE promoter and a reduction of Irf4 protein in CpG ODN-treated B cells. These data indicate the critical role of NFκB and Irf4 in the regulation of IgE CSR through actions downstream of CpG signaling. Unexpectedly, STAT6 activity was not markedly changed by CpG ODN treatment. These results indicate that CpG signals work in various ways to induce Th1-skewed immune responses involving B cells.
|
Report
(3 results)
Research Products
(34 results)
-
-
-
[Journal Article] A transmembane chemokine, CXC chemokine ligand 16, expressed by lympho node fibroblastic reticular cells has the potential to regulate T cell migration and adhesion.2006
Author(s)
Hara, T., Katakai, T., Lee, J.H., Nambu, Y., Nagata, N.N., Gonda, H., Sugai, M., Shimizu, A.
-
Journal Title
International Immunology 18・2
Pages: 301-311
Related Report
-
-
[Journal Article] CpG inhibits IgE class switch recombination through suppression of NFkB activity, but not through Id2 or bc16.2005
Author(s)
Sugai, M.^<#+>, Kusunoki T.^#, Gonda H., Nambu Y., Nakajima-Nagata N., Katakai T., Kusunoki M., Sakamoto A., Tokuhisa T., Nakahata T., Yokota Y., Shimizu A.
-
Journal Title
Biochem. Biophys. Res. Commun. 11・328
Pages: 499-506
Description
「研究成果報告書概要(和文)」より
Related Report
-
-
-
-
[Journal Article] Polymorphisms in PTCH1 affect the risk of ameloblastoma.2005
Author(s)
Kawabata, T., Takahashi, K., Sugai, M., Murashima, A., Ando, S., Shimizu, A., Kosugi, S., Sato, T., Nishida, M., Murakami, K., Iizuka, T.
-
Journal Title
J. Dent. Res. 84・9
Pages: 812-6
Description
「研究成果報告書概要(和文)」より
Related Report
-
-
[Journal Article] CpG inhibits IgE class switch recombination through suppression of NFkB activity, but not through Id2 or bcl6.2005
Author(s)
Sugai M.^#, Kusunoki T.^#, Gonda H., Nambu Y., Nakajima-Nagata N., Katakai T., Kusunoki M., Sakamoto A., Tokuhisa T., Nakahata T., Yokota Y., Shimizu A.
-
Journal Title
Biochem.Biophys.Res.Commun. 11(328)
Pages: 499-506
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
-
-
[Journal Article] Polymorphisms in PTCH1 affect the risk of ameloblastoma.2005
Author(s)
Kawabata, T., Takahashi, K., Sugai, M., Murashima, A., Ando, S., Shimizu, A., Kosugi, S., Sato, T., Nishida, M., Murakami, K., Iizuka, T.
-
Journal Title
J.Dent.Res. 84(9)
Pages: 812-816
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
