| Project/Area Number |
16590234
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
KANOH Hideo Sapporo Medical University School of Medicine, Department of Biochemistry, Professor, 医学部, 教授 (70045475)
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| Co-Investigator(Kenkyū-buntansha) |
SAKANE Fumio Sapporo Medical University School of Medicine, Department of Biochemistry, Associate Professor, 医学部, 助教授 (10183815)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Diacylglycerol / Diacylglycerol kinase / Lipid phosphate phosphatase / Phosphatidic acid / Phosphatidic acid phosphatase / Signal transduction / ジアシルグリセロール キナーゼ / 脂質性メデイエーター / 細胞情報伝達 / リゾホスファチジン酸 |
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| Research Abstract |
We investigated the function of two lipid-metabolizing enzymes, diacylglycerol kinase (DGK) and lipid phosphate phosphatase (LPP), expressed in various cells. DGK gamma was found to act as a suppressor of Rac-1, thus inhibiting lamellipodium formation in fibroblasts. This DGK isozyme was also translocated into the nuclei of various cells, resulting in an inhibition of cell growth. The PH domain of DGK delta was suggested to be involved in the plasma membrane translocation of this isozyme. The tenth member of DGK isozyme, DGK kappa, was cloned and its enzymatic properties were characterized. In the case of LPP, which acts at the outer cell surface, we found that the expression of LPP-1 or LPP-3 caused a marked suppression of the growth of various ovarian cancer cell lines, suggesting the importance of metabolizing extracellular lyso-phosphatidic acid in cancer cell proliferation.
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