• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study of New Roles in AMP Metabolism by Using Gene Modified Animals

Research Project

Project/Area Number 16590259
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

MORISAKI Takayuki  National Cardiovascular Center Research Institute, Department of Bioscience, Director, バイオサイエンス部, 部長 (30174410)

Co-Investigator(Kenkyū-buntansha) MORISAKI Hiroko  National Cardiovascular Center Research Institute, Department of Bioscience, Laboratory Chief, バイオサイエンス部, 室長 (40311451)
HIDAKA Kyoko  National Cardiovascular Center Research Institute, Department of Bioscience, Laboratory Chief, バイオサイエンス部, 室長 (00216681)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsnucleotide metabolism / AMP deaminase / metabolic disorder / myopathy / disease model / gene modified animal / AMP / モデル動物 / 遺伝子破壊
Research Abstract

AMP deaminase (AMPD) catalyzes AMP to IMP and is thought to play an important role in purine metabolism, though detailed function of AMPD in vivo has not been understood. Therefore, we established gene knock-out mice for each AMPD gene to elucidate in vivo function of three AMPD genes. Furthermore, we tried to produce model mice for combined AMPD deficiency by mating these AMPD knock-out mice. AMPD2 knockout mice showed muscle specific deficiency of AMPD and increased AMP/ATP ratio in skeletal muscle as well as more phophorylation of AMP-activated kinase in muscle. AMPD2 knockout mice exhibited proteinuria along with decreased AMPD activity in kidney and liver. Also, they showed increased AMP and increase of phophorylated AMPK and ACC in liver. AMPD3 knockout mice exhibited decreased AMPD acitivity in red cells and heart, and they showed increased ATP in red cells without other prominent phenotype. About combined AMPD deficiency, AMPD2/AMPD3 double deficient mice were found to die before 3 weeks after birth, though AMPD1/AMPD2 double deficient mice as well as AMPD1/AMPD3 double deficient mice were established. These studies revealed the functional relevance of AMPD in kidney as well as liver.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (6 results)

All 2005 2004

All Journal Article (4 results) Book (2 results)

  • [Journal Article] Stable and uniform gene suppression by site-specific integration of siRNA expression cassette in murine embryonic stem cells.2005

    • Author(s)
      Zheng GD, Hidaka K, Morisaki T
    • Journal Title

      Stem Cells 23

      Pages: 1028-1034

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Haplotype Analysis of Human AMPD1 Gene. Origin of Common Mutant Allele.2004

    • Author(s)
      Toyama K et al.
    • Journal Title

      J Med Genet 41

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Haplotype Analysis of Human AMPD1 Gene. Origin of Common Mutant Allele.2004

    • Author(s)
      Toyama K et al.
    • Journal Title

      Journal of Medical Genetics 41

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Haplotype Analysis of Human AMPD1 Gene. Origin of Common Mutant Allele2004

    • Author(s)
      Toyama K et al.
    • Journal Title

      J Med Genet 41

    • Related Report
      2004 Annual Research Report
  • [Book] 高尿酸血症と痛風 : AMPデアミナーゼと循環器疾患2005

    • Author(s)
      森崎隆幸
    • Total Pages
      5
    • Publisher
      メディカルビュー社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 高尿酸血症と通風:AMPデアミナーゼと循環器疾患2005

    • Author(s)
      森崎 隆幸
    • Total Pages
      5
    • Publisher
      メディカルビュー社
    • Related Report
      2005 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi