Molecular pathological analysis of the ghrelin expression in H. Pylori associated diseases.
| Project/Area Number |
16590283
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Nagasaki University |
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Principal Investigator |
SEKINE Ichiro Nagasaki University, Graduate School of Biomedical Sciences, professor, 大学院・医歯薬学総合研究科, 教授 (60039922)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAYAMA Toshiyuki Nagasaki University, Graduate School of Biomedical Sciences, lecturer, 大学院・医歯薬学総合研究科, 講師 (30284673)
NAKASHIMA Masahiro Nagasaki University, Graduate School of Biomedical Sciences, lecturer, 大学院・医歯薬学総合研究科, 講師 (50284683)
OHTSURU Akira Nagasaki University, Hospital of Medicine and Dentistry, associate professor, 医学部・歯学部附属病院, 助教授 (00233198)
山下 俊一 長崎大学, 大学院・医歯薬学総合研究科, 教授 (30200679)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Ghrelin / Gastretomy / H.Pylori / Gastritis / Appetite / Growth hormon / IGF-I / BMI |
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| Research Abstract |
There are contradictory reports on the relationship between Helicobacter pylori and circulating ghrelin. We sought to clarify the influence of H.pylori infection on gastric and plasma ghrelin dynamics in humans. METHODS : Using endoscopic biopsies from the corpus of 56 H.pylori-infected patients and 25 uninfected subjects, ghrelin mRNA expression levels and gastric ghrelin peptide contents were measured by real-time polymerase chain reaction and radioimmunoassay, respectively. We also measured plasma ghrelin concentrations and analyzed the numbers of ghrelin immunoreactive cells in the fundic gland area. Fifty-one patients with H. pylori infection were treated with a 7-day triple therapy consisting of lansoprazole, clarithromycin, and amoxicillin. RESULTS : The gastric ghrelin mRNA expression level of H.pylori-positive patients (1.64 +/- 1.27 in arbitrary units) was significantly lower than in H.pylori-negative subjects (4.87 +/- 4.1, p<0.0001). A similar trend was noted for ghrelin pe
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ptide contents (31.2 +/- 27.5 vs 81.2 +/- 64.1 ng/mg protein, respectively, p < 0.0001). There was no significant difference in the number of ghrelin immunoreactive cells/mm^2 in terms of H. plyori status. Plasma ghrelin concentrations in H.pylori-infected patients (144.6 +/- 7.8.8 fmol/ml) were significantly lower than in uninfected subjects (196.1 +/- 97.2, p < 0.05) and increased following cure of the infection. Plasma ghrelin levels correlated positively with the expression levels of ghrelin mRNA (r=0.583, p<0.0001) and peptide products (r=0.574, p<0.0001). There was a significant stepwise decrease in gastric ghrelin mRNA expression (p<0.05), peptide contents (p<0.01) and density of ghrelin immunoreactive cells (p<0.05) with progression of histological severity of glandular atrophy in the corpus. The histological severity of chronic inflammation also negatively influenced the ghrelin mRNA expression (p<0.001) and peptide production (p<0.005). CONCLUSIONS : H.pylori infection has a negative impact on gastric and plasma ghrelin dynamics. Chronic inflammatory and atrophic changes associated with the infection may affect gastric ghrelin biosynthesis and contribute to the low circulating levels.
The patient with total gastrectomy or severe atrophic gastritis shows body weight reduction, anorexia, osteomalasia, etc. The ghrelin supply treatment might be beneficial effect for above symptoms as a kind of somatopause Less
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Report
(3 results)
Research Products
(18 results)
