On the relationship between microdysgenesis in the hippocampus of Ihara epileptic rat and its epileptogenesis

• Project/Area Number: 16590310
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Kyoto University
• Principal Investigator: AMANO Shigeru Kyoto University, Facullty of Medicine, Professor, 医学部, 教授 (50111886)
• Co-Investigator(Kenkyū-buntansha): MIKUNI Nobuhiro Kyoto University, Post graduate school of Medicine, Assistant, 医学研究科, 助手 (60314217)
• Project Period (FY): 2004 – 2005
• Project Status: Completed (Fiscal Year 2005)
• Budget Amount: ¥3,400,000 (Direct Cost: ¥3,400,000); Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000); Fiscal Year 2004: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: Ihar Epileptic Rat / Model animal / Micorodysgenesis / Eoileotogenesis / Neuropathology / てんかん原生

Research Abstract

This research project, "On the relationship between micorodysgensis in the hippocampus of Ihara Epileptic Rat (IER) and its epilentogenesis" was performed by two step experiments.
In first step, mating study where IER was mated with non-epileptic rat (Wistar rat) to obtain F1 generation was programmed. Then, female F1 generation was mated by male IER to obtain back cross rats. These back cross animals were monitored after 5 months old every one month till 10 months old by automated seizure monitoring system, to define the rat has seizures or not. After two hundred and forty back cross rats were monitored, fourteen animals was defined to have experience of seizures. The incidence of seizure development in back cross generation is 4.8%.
In the second step, histological investigation of the brains of whole back crosses animals. In the back cross rats which experienced seizures, micordysgenesis in the hippocampus is observed in 8 rats among 14 seizures rat, Thus positive ratio for microdysgenesis is 57%. In the back cross rats which has no seizure, on the other hand, micordysgenesis in the hippocampus is observed in 22 rats among 50 non- seizures rat, Thus positive ratio for microdysgenesis is 44%.
These data show that microdysgenesis in the IER hippocampus has no direct relationship between morphological change in the hippocampus and epileptogenesis in this epilepsy model rats.

Research Products

• [Journal Article] Open-field behaviors and water-maze learning in the F substrain of Ihara epileptic ratas (2006)
  • Author(s): Okaichi Y., Amano S., Ihara N., Hayase Y., Tazumi T., Okaichi H.
  • Journal Title: Epilepsia 47(1) Pages: 55-63
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Open-field bahaviora and water-maze learning in the F aubstrain of Ihara epileptic rata (2006)
  • Author(s): Okaichi Y., Amano S., Ihara N., Hayase Y., Tazusi T., Okaichi H.
  • Journal Title: Epilepsia 47 Pages: 55-63
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Open field behaviors and water maze learning in Ihara epileptic rats, (2006)
  • Author(s): Okaichi, Y., Amano, S., Ihara, N., Hayase, Y., Tazumi, T., Okaichi, H.
  • Journal Title: Epilepsia 47 Pages: 55-63
• [Journal Article] A non-specific form of dysembrionic neuroepithelial tumor presenting with intractable epilepsy (2005)
  • Author(s): Nishida N., Hayase Y., Mikuni N., Enatsu R., Hayashi., N., Takahashi JA., Nakashima Y., Ikeda A., Anano S., Hashgimoto N.
  • Journal Title: Brain tumor pathol 22 Pages: 35-40
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A nonsepcific form of dysembryoplastic neuroepithelial tuomor presenting with intractable epilepsy. (2005)
  • Author(s): Nishida N., Hayaae Y., Mikuni N., Enatau R., Hayaahi N., Takahashi JA., Nakashima Y., Ikeda A., amano S., Hashimoto N.
  • Journal Title: Brain Tumor Pathol. 22 Pages: 35-40
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Surgical resection of an epileptogenetic cortical dysplasia in deep foot sensomortor area : a case report
  • Author(s): Mikuni N., Ikeda A., Hauase Y., Amano s., Hanakawa T., Fukuyama H., Hashimoto N.
  • Journal Title: Epilepsia and behavior (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Surgical resection of an epileptic cortical dysplasia in the deep sensomortor area : a case report
  • Author(s): Mikuni N., Ikeda A., Hayase Y., Aaano S., Hanaoka T., Fukuyama H., Hashimoto N.
  • Journal Title: Epilepsy and Behavior (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Surgical resection of an epileptogenic cortical dysplasia in the deep foot sensorimotor area : a case repot
  • Author(s): Mikuni N., Ikeda A., Hayase Y., Amano S., Hanakawa T., Fukuyama H., Hashimoto N.
  • Journal Title: Epilepsy and Behavior (in press)(印刷中)