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Search for novel molecule response to oxidative stress

Research Project

Project/Area Number 16590326
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionTokai University

Principal Investigator

TAKEKOSHI Susumu  Tokai Univ., School of Medicine, Associate Professor, 医学部, 助教授 (70216878)

Co-Investigator(Kenkyū-buntansha) TAKIZAWA Shunya  Tokai Univ., School of Medicine, Associate Professor, 医学部, 助教授 (70197234)
OSAMURA Yoshiyuki  Tokai Univ., School of Medicine, Professor, 医学部, 教授 (10100992)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsprotein kinase C / diacylglycerol / oxidative stress / lipid peroxidation / neuron / brain / signal transduction / MAP kinase
Research Abstract

Protein kinase C (PKC) is an unique enzyme which is activated by calcium ion and lipids such as phosphatidylserine, 1,2-diacylglycerol, free fatty acids, and lysophosphatidylcholine. It is well known that PKC plays a crucial role in receptor-mediated signal transduction affecting diverse range of cellular responses such as cell proliferation, differentiation, hormonal action and neurotransmission. On the other hand, various reports indicate that PKC molecules also participate in pathological processes such as tumor promotion, inflammation, autoimmune diseases, atherosclerosis and neurodegenerative disorder. We have demonstrated that oxidized-diacylglycerol (DAG-OOH) activates rat brain PKC as efficiently as phorbol ester (PMA), artificial and powerful activator for PKC. This result markedly suggested that DAG-OOH might act as a biological messenger in oxidative stress to efficiently alter the PKC-dependent signal transduction system to a similar extent to PMA. Furthermore, DAG-OOH injured the cultured neurons with the over-activation of PKC delta and MAP kinase. Among these studies, it has been suspected that unknown PKC isoforms (PKC-X), which is more susceptible to DAG-OOH than other PKC isoforms, exist in rat brain homogenate. PKC-X is thought to be the importance role for the DAG-OOH-induced neuronal cell injury. In the present study, we attempt to identify PKC-X from rat brain homogenate. The fraction that had a high concentration of PKC-X protein was separated from brain homogenate by DEAE-sephacel column chromatography. Using Biacore device that be able to analyze the molecular- molecular interaction, it became apparent that this fraction highly containing PKC-X exhibited particular affinity to DAG-OOH.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (36 results)

All 2006 2005 2004 Other

All Journal Article (36 results)

  • [Journal Article] Functional and morfphological changes in the hypothalamus-pituitary posterior lobe system after hypophysectomy in the dog2006

    • Author(s)
      Takahiro Taoda, et al.
    • Journal Title

      J Vet Med Sci 68

      Pages: 1-7

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Functional and morphological changes in the hypothalamus-pituitary posterior lobe system after hypophysectomy in the dog.2006

    • Author(s)
      Takahiro Taoda, et al.
    • Journal Title

      J Vet Med Sci 68

      Pages: 1-7

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Dietary soybean enhances Pit-1 dependent pituitary hormone production in iodine defficient rats.2005

    • Author(s)
      Hanako Kajiya, et al.
    • Journal Title

      Journal of Molecular Histology 36

      Pages: 263-272

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular and histological studies of pituitary tumorigenesis using experimental animal models.2005

    • Author(s)
      Takeo Minematsu, et al.
    • Journal Title

      Acta Histochemica Cytochemica 38

      Pages: 87-92

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Pit-1 Gene Inhibition Using Small Interfering RNAs in Rat Pituitary GH Secreting Cell Line2005

    • Author(s)
      Shunsuke Miyai, et al.
    • Journal Title

      Acta Histochemica Cytochemica 38

      Pages: 107-114

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Induction of GH, PRL, and TSHbeta mRNA by transfection of Pit-1 in a human pituitary adenoma-derived cell line.2005

    • Author(s)
      Shunsuke Miyai, et al.
    • Journal Title

      Cell Tissue Research 322

      Pages: 269-277

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Three-dimensional Imaging of the Intracellular Localization of GH and Prolactin and Their mRNA Using Nanocrystal (Quantum Dot) and CLSMi Techniques.2005

    • Author(s)
      Akira Matsuno, et al.
    • Journal Title

      J Histochemistry Cytochemistry 53

      Pages: 833-838

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Estrogen receptor-negative and human epidermal growth factor receptor 2-negative breast cancer tissue have the highest Ki-67 labeling index and EGFR expression2005

    • Author(s)
      Shinobu Umemura, et al.
    • Journal Title

      Oncology Reports 14

      Pages: 337-343

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Histochemical technologies for genomics and proteomics : Laser capture microdissection (LCM) and tissue mocroarray (TMA)2005

    • Author(s)
      Robert Y.Osamura, et al.
    • Journal Title

      Acta Histochemica Cytochemica 38

      Pages: 185-188

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Two or three dimensional imagings of simultaneous visualization of rat pituitary hormone and its mRNA2005

    • Author(s)
      Akira Matsuno, et al.
    • Journal Title

      Acta Histochemica Cytochemica 38

      Pages: 253-256

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Establishment of Stable GH3 Cell Line Expressing Enhanced Yellow Fluorescein Protein (EYFP)-Growth Hormone (GH) Fusion Protein2005

    • Author(s)
      Akira Matsuno, et al.
    • Journal Title

      J Histochemistry Cytochemistry 53

      Pages: 1177-1180

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Purified from cultured safflower cells, is a novel and potent singlet oxygen quencher2005

    • Author(s)
      Yasuhiro Kambayashi, et al.
    • Journal Title

      Acta Biochimica Polonica 52

      Pages: 903-908

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Recent progress in studies of pituitary tumor pathogenesis2005

    • Author(s)
      Takeo Minematsu, et al.
    • Journal Title

      Endocrine 28

      Pages: 37-41

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Dietary soybean enhances Pit-1 dependent pituitary hormone production in iodine deficient rats2005

    • Author(s)
      Hanako Kajiya, et al.
    • Journal Title

      Journal of Molecular Histology 36

      Pages: 263-272

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Pit-1 Gene Inhibition Using Small Interfering RNAs in Rat Pituitary GH Secreting Cell Line.2005

    • Author(s)
      Shunsuke Miyai, et al.
    • Journal Title

      Acta Histochemica Cytochemica 38

      Pages: 107-114

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Induction of GH,PRL, and TSHbeta mRNA by transfection of Pit-1 in a human pituitary adenoma-derived cell line.2005

    • Author(s)
      Shunsuke Miyai, et al.
    • Journal Title

      Cell Tissue Research 322

      Pages: 269-277

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Three-dimensional Imaging of the Intracellular Localization of Growth Hormone and Prolactin and Their mRNA Using Nanocrystal (Quantum Dot) and Confocal Laser Scanning Microscopy Techniques.2005

    • Author(s)
      Akira Matsuno, et al.
    • Journal Title

      J Histochemistry Cytochemistry 53

      Pages: 833-838

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Histochemical technologies for genomics and proteomics : Laser capture microdissection (LCM) and tissue microarray (TMA).2005

    • Author(s)
      Robert Y.Osamura, et al.
    • Journal Title

      Acta Histochemica Cytochemica 38

      Pages: 185-188

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Kinobeon A, Purified from cultured safflower cells, is a novel and potent singlet oxygen quencher.2005

    • Author(s)
      Yasuhiro Kambayashi, et al.
    • Journal Title

      Acta Biochimica Polonica 52

      Pages: 903-908

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Dietary soybean enhances Pit-1 dependent pituitary hormone production in iodine deficient rats.2005

    • Author(s)
      Kajiya H., Takekoshi S., et al.
    • Journal Title

      Journal of Molecular Histology 36

      Pages: 263-272

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Molecular and histological studies of pituitary tumorigenesis using experimental animal models.2005

    • Author(s)
      Minematsu T., Takekoshi S., et al.
    • Journal Title

      Acta Histochemica Cytochemica 38

      Pages: 87-92

    • NAID

      110003161233

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Pit-1 Gene Inhibition Using Small Interfering RNAs in Rat Pituitary GH Secreting Cell Line.2005

    • Author(s)
      Miyai S., Takekoshi S., et al.
    • Journal Title

      Acta Histochemica Cytochemica 38

      Pages: 107-114

    • NAID

      110003161236

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Induction of GH, PRL, and TSHbeta mRNA by transfection of Pit-1 in a human pituitary adenoma-derived cell line.2005

    • Author(s)
      Miyai S., Takekoshi S., et al.
    • Journal Title

      Cell Tissue Research 322

      Pages: 269-277

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Three-dimensional Imaging of the Intracellular Localization of Growth Hormone and Prolactin and Their mRNA Using Nanocrystal (Quantum Dot) and Confocal Laser Scanning Microscopy Techniques.2005

    • Author(s)
      Matsuno A., Takekoshi S., et al.
    • Journal Title

      J Histochemistry Cytochemistry 53

      Pages: 833-838

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Estrogen receptor-negative and human epidermal growth factor receptor 2-negative breast cancer tissue have the highest Ki-67 labeling index and EGFR expression2005

    • Author(s)
      Umemura S., Takekoshi S., et al.
    • Journal Title

      Oncology Reports 14

      Pages: 337-343

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Histochemical technologies for genomics and proteomics : Laser capture microdissection (LCM) and tissue microarray (TMA)2005

    • Author(s)
      Osamura Y., Takekoshi S., et al.
    • Journal Title

      Acta Histochemica Cytochemica 38

      Pages: 185-188

    • NAID

      110003161317

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Two or three dimensional imagings of simultaneous visualization of rat pituitary hormone and its mRNA2005

    • Author(s)
      Matsuno A., Takekoshi S., et al.
    • Journal Title

      Acta Histochemica Cytochemica 38

      Pages: 253-256

    • Related Report
      2005 Annual Research Report
  • [Journal Article]2005

    • Author(s)
      Matsuno A., Takekoshi S., et al.
    • Journal Title

      J Histochemistry Cytochemistry 53

      Pages: 1177-1180

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Kinobeon A, Purified from cultured safflower cells, is a novel and potent singlet oxygen quencher.2005

    • Author(s)
      Kambayashi Y., Takekoshi S., et al.
    • Journal Title

      Acta Biochimica Polonica 52

      Pages: 903-908

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Recent progress in studies of pituitary tumor pathogenesis.2005

    • Author(s)
      Minematsu T., Takekoshi S., et al.
    • Journal Title

      Endocrine 28

      Pages: 37-41

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Production of pituitary hormone by human pituitary adenoma is under autocrine and paracrine regulationof hypothalamic hormones secreted from adenoma cells2004

    • Author(s)
      Matsuno A., Takekoshi S., et al.
    • Journal Title

      Acta Histochemca Cytochemica 36

      Pages: 405-420

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Modification of p53 immunoexpression associated with chemotherapy regimens in advanced and refractory ovarian cancers2004

    • Author(s)
      Hirasawa Y., Takekoshi S., et al.
    • Journal Title

      Acta Histochemca Cytochemica 37

      Pages: 15-20

    • NAID

      110003144312

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Quercetin Enhances Melanogenesis by Increasing Activity and Synthesis of Tyrosinase in Human Melanoma Cells2004

    • Author(s)
      Nagata H., Takekoshi S., et al.
    • Journal Title

      Pigment Cell Research 17

      Pages: 66-73

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cocaine- and amphetamine-regulated transcript peptide in the rat anterior pituitary gland is localized in gonadotrophs and suppresses prolactin secretion2004

    • Author(s)
      Kuriyama G., Takekoshi S., et al.
    • Journal Title

      Endocrinology 2004

      Pages: 2542-2550

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Quercetin induced melanogenesis in a reconstituted three-dimensional human epidermal model2004

    • Author(s)
      Takeyama R., Takekoshi S., et al.
    • Journal Title

      Journal of Molecular Histology 35

      Pages: 157-165

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Implication of genetic alterations caused by progesterone administration in endometrial hyperplasia and adenocarcinoma

    • Author(s)
      Yasuda M., Takekoshi S., et al.
    • Journal Title

      International Journal of Gynecological Cancer (In press)

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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