| Project/Area Number |
16590330
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
OKAWARA Akiko National Institute of Infectious Diseases, Senior Researcher, 生物活性物質部, 主任研究官 (30260277)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Kazuo National Institute of Infectious Diseases, Bioactive Molecules, Laboratory head, 室長 (20192130)
NAKAYAMA Toshinori Chiba University, Graduate School of Medicine, Department of Immunology, Professor, 大学院・医学研究院, 教授 (50237468)
OHNO Naohito Tokyo University of Pharmacy and Life Science, Laboratory for Immunopharmacology of Microbial Products, School of pharmacy, Professor, 薬学部, 教授 (80152213)
MINAMITANI Haruyuki Keio University, Graduate School of Science and Technology, Institute of Biomedical Engineering, Professor, 理工学部, 教授 (70051779)
KAMEOKA Yosuke National Institute of Biomedical Innovation, Laboratory of Genetic Resources, Senior Researcher, 生物資源研究部, 主任研究員 (00224692)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | neutrophil / MPO-ANCA / nephritis / vasculitis / QD |
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| Research Abstract |
We examined the role of myeloperoxidase (MPO) and the Antibody (Ab) to MPO in the pathogenesis of glomerulonephritis associated with MPO-specific anti-neuttophil cytoplasmic auto-Ab (MPO-ANCA) in experimental glomerulonephritis mice using quantum dots (QDs). We demonstrated the QD-conjugated anti-MPO Ab visualized the expression of MPO on the neutrophil surface after stimulation with proinflammatory cutokines such as IL-1β and TNF-α and FMLP. It is notable that the spontaneous crescentic glomerulonephritis model mouse (SCG/Kj mice) showed the expression of MPO translocation to the surface even in resident state. Moreover, we also observed that MPO translocation to the surface of neutrophils in patients with rapid progressive glomerulonephritis without any stimulation, suggesting that MPO translocation may be certain to contribute to the development of glomerular lesion. These results indicate that the expressed MPO on the activated neutrophils with anti-MPO Ab may coordinately play essential roles in the initial steps for the development of glomerulonephritis.
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