| Project/Area Number |
16590340
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Nagasaki University |
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Principal Investigator |
YUI Katsuyuki Nagasaki University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (90274638)
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| Co-Investigator(Kenkyū-buntansha) |
HONMA Kiri Nagasaki University, Graduate School of Biomedical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 講師 (70307940)
MIYAKODA Mana Nagasaki University, Graduate School of Biomedical Sciences, Research Associate, 大学院・医歯薬学総合研究科, 助手 (30398151)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | malaria / T cells / memory cells / dendritic cells / cytokine / macrophage / pattern recognition receptor / immune regulation / スポロゾイト / 防御免疫 |
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| Research Abstract |
1.Modulation of T-cell immune responses by malaria parasites
We studied immune responses and the protective effect of CD4^+ T-cells in mice recovered from P.yoelii 17XNL infection. CD4^+ T-cells from spleen of mice recovered from malaria infection showed spesific proliferative responses. However, they produced limited levels of IL-2 and high levels of IL-10. Adoptive transfer of these purified CD4^+ T-cells could not confer protective immunity against sporozoite infection. These results suggested that CD4^+ T-cells in Plasmodium-infected mice are functionally modulated.
2.Function of dendritic cells in malaria-infected mice
We characterized function of dendritic cells from mice infected with P.yoelii. Production of IL-12 in response to Toll-like receptor stimulation was reduced when compared with normal dendritic cells while IL-10 production was augmented. The ability to activate antigen specific T-cells was not reduced in both MHC class I and class II pathways. We will continue to study molecular mechanisms underlying augmented IL-10 production and function of T-cells activated by these dendritic cells.
3.Function of IRF-4, transcription factor that regulates immune responses, in dendritic cells and macrophages
Transcription factor IRF-4 is expressed in immune cells including lymphocytes, dendritic cells and macrophages. We have revealed that IRF-4 plays a critical role for the differentiation of a subset of dendritic cells and has inhibitory role for the TLR-mediated signaling. This inhibition mainly regulates activation of MAP kinase JNK and NF-κB.
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