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Development of a rapid molecular diagnostic method for malaria at endemic villages

Research Project

Project/Area Number 16590341
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Parasitology (including Sanitary zoology)
Research InstitutionOITA UNIVERSITY

Principal Investigator

KAWAMOTO Fumihiko  OITA UNIVERSITY, Inst.Scientific Research, Prof., 総合科学研究支援センター, 教授 (40115556)

Co-Investigator(Kenkyū-buntansha) KANBE Toshio  Nagoya Univ., Grad.Sch., Lecturer, 大学院・医学系研究科, 講師 (50093018)
OTSUKA Yasushi  OITA UNIVERSITY, Faculty of Medicine, Research Assoc., 医学部, 助手 (00244161)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsMalaria / Molecular diagnosis / Rapid diagnosis / Simple diagnosis / Lamp method / Topo-isomerase II / Gene analysis / Rapid DNA extraction
Research Abstract

Molecular diagnosis for malaria by Nested PCR method is highly sensitive, but it is impossible to apply at the fields of malaria endemic areas. Recently, a new molecular amplification method (Lamp method) was reported. This method is simple and DNA is easily amplified only by incubating reagents with at 63 C. Furthermore, amplified product can be detected by naked eyes without any equipment. Therefore, this study was aimed to develop a new molecular diagnosis for malaria using the Lamp method.
Primer sets were designed from a target sequence in 18S ribosomal RNA gene. Using this set, DNA of malaria parasites was amplified greatly and white precipitates was seen in the bottom of the reaction tubes within 30 min to 1 hrs, indicating that malaria diagnosis was possible by this method. Application of this method in a field at Flores island, Indonesia, was also successful and detection lebel was the same with a nested PCR diagnosis.
For speciation of the four malaria species, it was impossible to design good primer sets from the 18S RNA gene because of too short target sequences. In order to use Topoisomerase II gene which has been used for speciation of yeasts and Candida, we have been analyzing its gene in malaria parasites and design primer sets for the speciation of the human malaria parasites.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (13 results)

All 2006 2005 2004

All Journal Article (13 results)

  • [Journal Article] Sequence variation in the T-cell epitopes of the Plasmodium falciparum circumsporzoite (CS) protein among field isolates is temporally stable : a five-year longitudinal study in Southern Vietnam.2006

    • Author(s)
      Jalloh, A. et al.
    • Journal Title

      J. Clin. Microbiol. (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Sequence variation in the T-cell epitopes of the Plasmodium falciparum circumsporozoite (CS) protein among field isolates is temporally stable : a five-year longitudinal study in Southern Vietnam.2006

    • Author(s)
      Jalloh, A.et al.
    • Journal Title

      J.Clin.Microbiol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Sequence variation in the T-cell epitopes of the Plasmodium falciparum circumsporozoite (CS) protein among field isolates is temporally stable: a five-year longitudinal study in Southern Vietnam.2006

    • Author(s)
      Jalloh, A. et al.
    • Journal Title

      J.Clin.Microbiol. (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Malaria endemic patterns on Lombok and Sumbawa islands, Imdonesia.2005

    • Author(s)
      Dachlan, Y. P. et al.
    • Journal Title

      Trop. Med. Health 33

      Pages: 105-113

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Glucose-6-phosphate dehydrogenase (G6PD) mutations in Camodia : G6PD viangchan (871G>A) is the most common variant in the Cambodian population.2005

    • Author(s)
      Matsuoka, H. et al.
    • Journal Title

      J. Human Genet. 50

      Pages: 468-472

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Malaria endemic patterns on Lombok and Sumbawa islands, Indonesia.2005

    • Author(s)
      Dachlan, Y.P.et al.
    • Journal Title

      Trop.Med.Health 33

      Pages: 105-113

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Glucose-6-phosphate dehydrogenase (G6PD) mutations in Cambodia : G6PD Viangchan (871G > A) is the most common variant in the Cambodian population.2005

    • Author(s)
      Matsuoka, H.et al.
    • Journal Title

      J.Human Genet. 50

      Pages: 468-472

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Malaria endemic patterns on Lombok and Sumbawa islands, Indonesia.2005

    • Author(s)
      Dachlan, Y.P. et al.
    • Journal Title

      Trop.Med.Health 33

      Pages: 105-113

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Glucose-6-phosphate dehydrogenase (G6PD) mutations in Cambodia: G6PD Viangchan(871G>A) is the most common variant in the Cambodian population.2005

    • Author(s)
      Matsuoka, H. et al.
    • Journal Title

      J.Human Genet. 50

      Pages: 468-472

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Molecular analysis of Plasmodium ovale variants.2004

    • Author(s)
      Win, T.T.et al.
    • Journal Title

      Emerg. Ing. Dis. 10

      Pages: 1235-1240

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular analysis of Plasmodium ovale variants.2004

    • Author(s)
      Win, T.T.et al.
    • Journal Title

      Emerg.Infec.Dis. 10

      Pages: 1235-1240

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular analysis of Plasmodium ovale variants.2004

    • Author(s)
      Win, T.T.et al.
    • Journal Title

      Emerg.inf.Dis. 10

      Pages: 1235-1240

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Molecular analysis of Plasmodium ovale variants.2004

    • Author(s)
      Win, T.T.et al.
    • Journal Title

      Emerg.Inf.Dis. 10

      Pages: 1235-1240

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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