Analysis of the effects of the disruption of the antioxidant protein gene on developments of malaria parasite in its mammalian and insect stages
| Project/Area Number |
16590351
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Research Institute, International Medical Center of Japan |
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Principal Investigator |
KAWAZU Shin-ichiro International Medical Center of Japan, Research Institute, Division Chief, 適正技術開発・移転研究部適正技術開発研究室, 室長 (60312295)
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| Co-Investigator(Kenkyū-buntansha) |
KANO Shigeyuki International Medical Center of Japan, Research Institute, Department Director, 適正技術開発・移転研究部, 部長 (60233912)
TSUBOI Takafumi Ehime University, Cell-Free Science and Technology Research Center, Professor, 無細胞生命科学工学研究センター, 教授 (00188616)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Infectious diseases / Malaria / Stress / Redox / Reverse genetics / Transgenic parasite / Mosquito stage / レドクス |
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| Research Abstract |
In this project, we investigated the physiologic role of typical 2-Cys peroxiredoxin in malaria parasites (TPx-1) by disrupting the gene in the rodent malaria parasite Plasmodium berghei. The gene-disrupted parasite (Prx KO) developed normally in mouse erythrocytes and multiplied at a rate similar to that of the parent strain (WT) during the experimental period. However, Prx KO produced up to 60% fewer gametocytes, sexual-stage parasites involved in the transition between the mammalian host and the mosquito, than WT did. Prx KO also showed defect in sporozoite production in its mosquito stage. These results suggest that TPx-1 is required for normal gametocyte and sporozoite development. Although the mechanism by which PbTPx-1 contributes to the parasite development remains unknown, these findings suggest, for the first time, the involvement of Prx in the sexual development of the malaria parasite. Further studies clarifying the roles of Prx will provide new insight into the biology of malaria parasites and facilitate development of strategies to interrupt their life cycle.
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Report
(3 results)
Research Products
(8 results)
