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Analysis of secretion and maturation pathway of bacterial proteintoxin and its application to clinical study

Research Project

Project/Area Number 16590360
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

OKAMOTO Keinosuke  Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (70131183)

Co-Investigator(Kenkyū-buntansha) MIYOSHI Shin-ichi  Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (60182060)
NAKAO Hiroshi  Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Associate Professor, 大学院・医歯薬学総合研究科, 助教授 (20237217)
YAMANAKA Hiroyasu  Hiroshima International University, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (30202386)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsBacteria / Aeromonas / Protease / Chaperone / Escherichia coli / Enterotoxin / Secretion apparatus / Pathogenicity / 溶血毒 / フーリン / 下痢 / 基質 / タンパク毒素 / 成熟化 / 溶血毒素 / 外膜蛋白質
Research Abstract

Bacterial pathogenicity of bacteria is often determined by the exotoxins produced from the bacteria. The loss of the ability to produce these toxins leads to the loss of pathogenicity. These extoxins are protein. The protein just synthesized in cytoplasm is a string composed of amino acid residues. In the process to become mature (bio-active) protein, many bacterial factors and apparatus are involved. If the bacteria lose the ability to produce these factors and apparatus, the bacteria cannot produce exotoxins. This leads the conversion of pathogenic bacteria to non-pathogenic bacteria. In addition, the compounds to inhibit the maturation process of proteintoxin might be unique and good medicine for these pathogenic bacteria. From this view point, the studies reported here were performed. The subjects treated in this experiments were serine protease of Aeromonas and heat-stable enterotoxin (ST) of Escherichia coli. The studies revealed the following points. The serine protease of Aerom … More onas requires the dependent chaperone protein to become mature toxin. The requirement of dependent chaperone protein for the maturation is rare in bacterial proteases. The subsequent studies showed that the association of the chaperone protein with the serine protease is achieved in periplasmic space. The mutation experiments suggested that the carboxy terminal regions of both proteins, chaperone and serine protease, are important for the association. The inhibition of the association might results in the loss of serine protease production for Aeromonas. The studies about the secretion of ST revealed the following points. An outer membrane protein, TolC, is utilized in the secretion of ST. Almost gram-negative bacteria possess TolC, however, the amino acid sequences of these TolCs slightly differ each other among these TolCs. To clear the relationship between the difference and its host strain, we expressed tolC gene of Vibrio parahaemolyticus in Escherichia coli and measured tits activity. The results showed that TolC of V.parahemolyticus expressed in is functional when it interacts with AcrAB, which is a apparatus for secretion located in inner membrane, however, TolC of V.parahemolyticus cannot cooperate with other secretion apparatuses in inner membrane of E.coli Of course, TolC of E.coli can cooperate with these secretion apparatuses. The amino acid residues, which are not common to sequences of both TolCs, may be important in the binding for TolC with these secretion apparatuses. Less

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (32 results)

All 2006 2005 2004

All Journal Article (32 results)

  • [Journal Article] Cleavage specificity of the serine protease of Aeromonas sobria, a member of the kexin family of subtilases2006

    • Author(s)
      Hidetomo Kobayashi
    • Journal Title

      FEMS Microbiology Letters 256(1)

      Pages: 165-170

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Placental and intestinal alkaline phosphatases are receptors for Aeromonas sobria hemolysin2005

    • Author(s)
      Akihiro Wada 他
    • Journal Title

      International Journal of Medical Microbiology 294(7)

      Pages: 427-435

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A pore-forming toxin produces by Aeromonas sobria activates cAMP-dependent CI^- secretory pathways to cause diarrhea2005

    • Author(s)
      Naomi Tanoue 他
    • Journal Title

      FEMS Microbiology Letters 242(2)

      Pages: 195-201

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A hemolysin of Vibrio mimicus (VMH) stimulates cells to produce ATP and cyclic AMP which appear to be secretory mediators2005

    • Author(s)
      Yunshan Li 他
    • Journal Title

      Microbiology and Immunology 49(1)

      Pages: 73-78

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A pore-forming toxin produced by Aeromonas sobria activates Ca^<2+> dependent Cl^<-> secretion2005

    • Author(s)
      Akira Takahashi 他
    • Journal Title

      Microbial Pathogenesis 38(4)

      Pages: 173-180

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Inhibitory effects of heterocyclic amine - induced DNA adduct formation in mouse liver and lung by Beer2005

    • Author(s)
      S.Arimoto-Kobayashi, 他
    • Journal Title

      J. Agric. Food Chem. 53(3)

      Pages: 812-815

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Evaluation of photo-mutagenicity and photo-cytotoxicity of food coloring agents.2005

    • Author(s)
      Sakae Arimoto-Kobayashi 他
    • Journal Title

      Mutagenesis 20(3)

      Pages: 229-233

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The cytotoxin-hemolysin genes of human and eel pathogenic Vibrio vulnificus strains : Comparison of nucleotide sequences and applications to the genetic grouping2005

    • Author(s)
      Mitsutoshi Senoh 他
    • Journal Title

      Microbiology and Immunology 49(6)

      Pages: 513-519

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Novel antimutagenic factors derived from the edible mushroom Agrocybe cylindracea2005

    • Author(s)
      K.Taria, 他
    • Journal Title

      Mutation Research 586

      Pages: 115-123

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Placental and intestinal alkaline phosphatases are receptors for Aeromonas sobria hemolysin.2005

    • Author(s)
      Akihiro Wada, Ai-ping Wang, Hajime Isomoto, Yoshinori Satomi, Toshifumi, Takao, Akira Takahashi, Shizuka Awata, Tomohiko Nomura, Yoshio Fujii, Shigeru Kohno, Keinosuke Okamoto, Joel Moss, Jose luis millan, Toshiya Hirayama
    • Journal Title

      International Journal of Medical Microbiology 297(7)

      Pages: 427-435

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A pore-forming toxin produced by Aeromonas sobria activates cAMP-dependent Cl^- secretory pathways to cause diarrhea.2005

    • Author(s)
      Naomi Tanoue, Akira Takahashi, Keinosuke Okamoto, Yoshio Fujii, Yutaka Taketani, Nagakatsu Harada, Masayuki Nakano, Yutaka Nakaya
    • Journal Title

      FEMS Microbiology Letters 242

      Pages: 195-201

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A hemolysin of Vibrio mimicus (VMH) stimulates cells to produce ATP and cyclic AMP which appear to be secretory mediators.2005

    • Author(s)
      Yunshan Li, Keinosuke Okamoto, Eizo Takahashi, Shin-ichi Miyoshi, Sumio Shinoda, Takao Tsuji, Yoshio Fujii
    • Journal Title

      Microbiology and Immunology 49(1)

      Pages: 73-78

    • NAID

      10014377277

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A pore-forming toxin produced by Aeromonas sobria activates Ca^<2+> dependent Cl^- secretion2005

    • Author(s)
      Akira Takahashi, Naomi Tanoue, Masayuki Nakano, Akiko Hamamoto, Keinosuke Okamoto, Yoshio Fujii, Nagakathu Harada, Yutaka Nakaya
    • Journal Title

      Microbial Pathogenesis 38(4)

      Pages: 173-180

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Inhibitory effects of heterocyclic amine-induced DNA adduct formation in mouse liver and lung by Beer2005

    • Author(s)
      Arimoto-Kobayashi S., Takata J., Nakandakari N., Fujioka R., Okamoto K., Konuma T.
    • Journal Title

      J.Agric.Food.Chem. 53(3)

      Pages: 812-815

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Evaluation of photo-mutagenicity and photo-cytotoxicity of food coloring agents.2005

    • Author(s)
      Sakae Arimoto-Kobayashi, Masaki Machida, Keinosuke Okamoto, Akie Yamaguchi
    • Journal Title

      Mutagenesis 20(3)

      Pages: 229-233

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The cytotoxin-hemolysin genes of human and eel pathogenic Vibrio vulnificus strains : Comparison of nucleotide sequences and applications to the genetic grouping.2005

    • Author(s)
      Mitsutoshi Senoh, Shin-ichi Miyoshi, Keinosuke Okamoto, Belen Fouz, Carmen Amaro, Sumio Shinoda
    • Journal Title

      Microbiology and Immunology 49(6)

      Pages: 513-519

    • NAID

      10016132140

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Novel antimutagenic factors derived from the edible mushroom Agrocybe cylindracea2005

    • Author(s)
      K.Taira, Y.Miyashita, K.Okamato, S.Arimoto, E.Takahashi, T.Negishi
    • Journal Title

      Mutatrion Research 586

      Pages: 115-123

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Placental and intestinal alkaline phosphatases are receptors for Aeromonas sobria hemolysin2005

    • Author(s)
      Akihiro Wada
    • Journal Title

      International Journal of Medical Microbiology 294(7)

      Pages: 427-435

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A pore-forming toxin produced by Aeromonas sobria activates cAMP-dependent Cl^- secretory pathways to cause diarrhea2005

    • Author(s)
      Naomi Tanoue
    • Journal Title

      FEMS Microbiology Letters 242(2)

      Pages: 195-201

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A hemolysin of Vibrio mimicus (VMH) stimulates cells to produce ATP and cyclic AMP which appear to be secretory mediators2005

    • Author(s)
      Yunshan Li
    • Journal Title

      Microbiology and Immunology 49(1)

      Pages: 73-78

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A pore-forming toxin produced by Aeromonas sobria activates Ca^<2+> dependent Cl^- secretion2005

    • Author(s)
      Akira Takahashi
    • Journal Title

      Microbial Pathogenesis 38(4)

      Pages: 173-180

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The cytotoxin-hemolysin genes of human and eel pathogenic Vibrio vulnificus strains : Comparison of nucleotide sequences and applications to the genetic grouping2005

    • Author(s)
      Mitsutoshi Senoh
    • Journal Title

      Microbiology and Immunology 49(6)

      Pages: 513-519

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A hemolysin of Vibrio mimicus (VMH) stimulates cells to produce ATP and cyclic AMP which appear to be secretory mediators2005

    • Author(s)
      李 云善
    • Journal Title

      Microbiology and Immunology 49・1

      Pages: 73-78

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A pore-forming toxin produced by Aeromonas sobria activates cAMP-dependent Cl- secretory pathways to cause diarrhea2005

    • Author(s)
      田ノ上 奈緒美
    • Journal Title

      FEMS Microbiology Letters 242・2

      Pages: 195-201

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Placental and intestinal alkaline phosphatases are receptors for Aeromonas sobria hemolysin2005

    • Author(s)
      和田 昭裕
    • Journal Title

      International Journal of Medical Microbiology 294・7

      Pages: 427-435

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Amino acid residues involved in the expression of the activity of Escherichia coli TolC2004

    • Author(s)
      Hiroyasu Yamanaka 他
    • Journal Title

      Microbiology and Immunology 48(10)

      Pages: 713-722

    • NAID

      10013604757

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Alternative methods to evaluate the protective ability of sunscreen against photo-genotoxicity2004

    • Author(s)
      Mwgumi Toyoshima 他
    • Journal Title

      Journal of Photochemistry and Photobiology B : Biology 73(1)

      Pages: 59-66

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Evaluation of photo-mutagenicity and photo-cytotoxicity of food coloring agents.2004

    • Author(s)
      Arimoto-Kobayashi S., 他
    • Journal Title

      The Japan Food Chemical Research Foundation, Research Reports 10

      Pages: 24-28

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Amino acid residues involved in the expression of the activity of Escherichia coli TolC.2004

    • Author(s)
      Hiroyasu Yamanaka, Naoyuki Morisada, Masaya Miyano, Hideaki Tsuge, Sumio Shinoda, Eizo Takahashi, Keinosuke Okamoto
    • Journal Title

      Microbiology and Immunology 48(10)

      Pages: 713-722

    • NAID

      10013604757

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Alternative methods to evaluate the protective ability of sunscreen against photo-genotoxicity.2004

    • Author(s)
      Megumi Toyoshima, Kanako Hosoda, Miho Hanamura, Keinosuke Okamoto, Hiroshi Kobayshi, Tomoe Negishi
    • Journal Title

      Journal of Photochemistry and Photobiology B : Biology 73(1)

      Pages: 59-66

    • NAID

      120002314085

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Evaluation of photo-mutagenicity and photo-cytotoxicity of food coloring agents.2004

    • Author(s)
      Arimoto-Kobayashi S., Machida M., Okamoto K., Yamaguchi, A
    • Journal Title

      The Japan Food Chemical Research Foundation, Research Reports 10

      Pages: 24-28

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Amino acid residues involved in the expression of the activity of Escherichia coli TolC2004

    • Author(s)
      山中浩泰
    • Journal Title

      Microbiology and Immunology 48・10

      Pages: 713-722

    • NAID

      10013604757

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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