Molecular mechanism of repressing IFN-mediated activity by viral interferon regulatory factor.
| Project/Area Number |
16590397
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | National Center for Child Health and Development |
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Principal Investigator |
NAKAMURA Hiroyuki National Center for Child Health and Development, Department of Infectious Diseases, Laboratory Head, 母児感染研究部, 室長 (70256866)
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| Project Period (FY) |
2004 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | HHV-8 / KSHV / vIRF1 / interferon / p53 / p53 / ATM |
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| Research Abstract |
Using human B-cell lines in which vIRF1 was expressed in a doxycycline-inducible manner, it was demonstrated that vIRF1 expression downregulates p53 protein level at the posttranslational level. It also was demonstrated that vIRF1 expression enhanced p53 cytoplasmic localization, resulting in an increase of mdm2-mediated p53 ubiquitination and degradation.
Furthermore, vIRF1 interacts with ATM kinase. The interaction leads to the inhibition of etoposide-induced ATM activation and p53 phosphorylation.
In summary, vIRF1 interacts with ATM and inhibits ATM/p53 signaling pathway.
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Report
(3 results)
Research Products
(10 results)
