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In vivo dynamics of EBNA1-oriP interaction during latent and lytic replication of Epstein-Barr virus.

Research Project

Project/Area Number 16590398
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionUniversity of Toyama (2006)
Aichi Cancer Center Research Institute (2004-2005)

Principal Investigator

DAIKOKU Tohru  University of Toyama, aboratory of Virology, Faculty of Medicine, Associate Professor, 医学薬学研究部ウイルス学, 助教授 (80291409)

Co-Investigator(Kenkyū-buntansha) 菅谷 豊  愛知県がんセンター(研究所), リサーチレジデント
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsEBV / EBNA1 / ChIP / Replication compartments / ミスマッチ修復
Research Abstract

The Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) is required for maintenance of the viral genome DNA during the latent phase of EBV replication but continues to be synthesized after the induction of viral productive replication. An EBV genome-wide chromatin immunoprecipitation assay revealed that EBNA1 constantly binds to oriP of the EBV genome during not only latent but also lytic infection. Although the total levels of EBNA1 proved constant throughout the latter, the levels of the oriP-bound form were increased as lytic infection proceeded. EBV productive DNA replication occurs at discrete sites in nuclei, called replication compartments, where viral replication proteins are clustered. Confocal laser microscopic analyses revealed that whereas EBNA1 was distributed broadly in nuclei as fine punctate dots during the latent phase of infection, the protein became redistributed to the viral replication compartments and localized as distinct spots within and/or nearby the compartments after the induction of lytic replication. Taking these findings into consideration, oriP regions of the EBV genome might be organized by EBNA1 into replication domains that may set up scaffolding for lytic replication and transcription.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (27 results)

All 2006 2005 2004

All Journal Article (27 results)

  • [Journal Article] Phosphorylation of MCM4 at sites inactivating DNA helicase activity of the MCM4-MCM6-MCM7 complex during Epstein-Barr virus productive replication.2006

    • Author(s)
      Ayumi Kudoh
    • Journal Title

      Journal of Virology Volume 80・No 20

      Pages: 10064-10072

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Establishment of a novel foreign gene delivery system combining an HSV amplicon with an attenuated replication-competent virus, HSV-1 HF102006

    • Author(s)
      Lumin Zhang
    • Journal Title

      Journal of Virological Methods Volume 137・No 2

      Pages: 177-183

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Postreplicative mismatch repair factors are recruited to Epstein-Barr virus replication compartments2006

    • Author(s)
      Tohru Daikoku
    • Journal Title

      Journal of Biological Chemistry Volume 281・No 16

      Pages: 11422-11430

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] EBVの潜伏感染と再活性化機構2006

    • Author(s)
      大黒 徹
    • Journal Title

      ヘルペスウイルス学-基礎・臨床研究の進歩- 64巻・増刊号3

      Pages: 584-588

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Phosphorylation of MCM4 at sites inactivating DNA helicase activity of the MCM4-MCM6-MCM7 complex during Epstein-Barr virus productive replication.2006

    • Author(s)
      Kudoh A, Daikoku T, Ishimi Y, Kawaguchi Y, Shirata N, Iwahori S, Isomura H, Tsurumi T.
    • Journal Title

      Journal of Virology. 80(20)

      Pages: 10064-10072

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Establishment of a novel foreign gene delivery system combining an HSV amplicon with an attenuated replication-competent virus, HSV-1 HF10.2006

    • Author(s)
      Zhang L, Daikoku T, Ohtake K, Ohtsuka J, Nawa A, Kudoh A, Iwahori S, Isomura H, Nishiyama Y, Tsurumi T.
    • Journal Title

      Journal of Virological Methods. 137(2)

      Pages: 177-1

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Postreplicative mismatch repair factors are recruited to Epstein-Barr virus replication compartments.2006

    • Author(s)
      Daikoku T, Kudoh A, Sugaya Y, Iwahori S, Shirata N, Isomura H, Tsurumi T.
    • Journal Title

      Journal of Biological Chemistry. 21;281(16)

      Pages: 11422-11430

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Latent infection and reactivation of Epstein-Barr virus2006

    • Author(s)
      Daikoku T
    • Journal Title

      Nippon Rinsho 64 Suppl

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] EBVの潜伏感染と再活性化機構2006

    • Author(s)
      大黒 徹
    • Journal Title

      ヘルペスウイルス学 -基礎・臨床研究の進歩- 64巻・増刊号3

      Pages: 584-588

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Postreplicative mismatch repair factors are recruited to Epstein-Barr virus replication compartments2006

    • Author(s)
      Tohru Daikoku
    • Journal Title

      The Journal of Biological Chemistry Vol 281・No16

      Pages: 11422-11430

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Two Spl/Sp3 binding sites in the major immediate-early proximal enhancer of human cytomegalovirus have a significant role in viral replication2005

    • Author(s)
      Hiroki Isomura
    • Journal Title

      Journal of Virology Volume 79・No 15

      Pages: 9597-9607

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Activation of ataxia telangiectasia-mutated DNA damage checkpoint signal transduction elicited by herpes simplex virus infection2005

    • Author(s)
      Noriko Shirata
    • Journal Title

      Journal of Biological Chemistry Volume 280・No 34

      Pages: 30336-30341

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Architecture of replication compartments formed during Epstein-Barr virus lytic replication2005

    • Author(s)
      Tohru Daikoku
    • Journal Title

      Journal of Virology Volume 79・No 6

      Pages: 3409-3418

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Epstein-Barr virus lytic replication elicits ATM checkpoint signal transduction while providing an S-phase-like cellular environment.2005

    • Author(s)
      Ayumi Kudoh
    • Journal Title

      Journal of Biological Chemistry Volume 280・No 9

      Pages: 8156-8163

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Two Sp1/Sp3 binding sites in the major immediate-early proximal enhancer of human cytomegalovirus have a significant role in viral replication.2005

    • Author(s)
      Isomura H, Stinski MF, Kudoh A, Daikoku T, Shirata N, Tsurumi T.
    • Journal Title

      Journal of Virology. 79(15)

      Pages: 9597-9607

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Activation of ataxia telangiectasia-mutated DNA damage checkpoint signal transduction elicited by herpes simplex virus infection.2005

    • Author(s)
      Shirata N, Kudoh A, Daikoku T, Tatsumi Y, Fujita M, Kiyono T, Sugaya Y, Isomura H, Ishizaki K, Tsurumi T.
    • Journal Title

      Journal of Biological Chemistry. 26;280(34)

      Pages: 30336-30341

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Architecture of replication compartments formed during Epstein-Barr virus lytic replication.2005

    • Author(s)
      Daikoku T, Kudoh A, Fujita M, Sugaya Y, Isomura H, Shirata N, Tsurumi T.
    • Journal Title

      Journal of Virology. 79(6)

      Pages: 3409-3418

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Epstein-Barr virus lytic replication elicits ATM checkpoint signal transduction while providing an S-phase-like cellular environment.2005

    • Author(s)
      Kudoh A, Fujita M, Zhang L, Shirata N, Daikoku T, Sugaya Y, Isomura H, Nishiyama Y, Tsurumi T.
    • Journal Title

      Journal of Biological Chemistry. 4;280(9)

      Pages: 8156-8163

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Architecture of replication compartments formed during Epstein-Barr virus lytic replication.2005

    • Author(s)
      Tohru Daikoku
    • Journal Title

      Journal of Virology 76(6)

      Pages: 3409-3418

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Architecture of replication compartments formed during Epstein-Barr virus lytic replication.2005

    • Author(s)
      Daikoku T, Kudoh A, Fujita M, Sugaya Y, Isomura H, Shirata N, Tsurumi T
    • Journal Title

      Journal of Virology 79(6)

      Pages: 3409-3418

    • Related Report
      2004 Annual Research Report
  • [Journal Article] In vivo dynamics of EBNA1-oriP interaction during latent and lytic replication of Epstein-Barr virus.2004

    • Author(s)
      Tohru Daikoku
    • Journal Title

      Journal of Biological Chemistry Volume 279・ No 52

      Pages: 54817-54825

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Inhibition of S-phase cyclin-dependent kinase activity blocks expression of Epstein-Barr virus immediate-early and early genes, preventing viral lytic replication.2004

    • Author(s)
      Ayumi Kudoh
    • Journal Title

      Journal of Virology Volume 78・No 1

      Pages: 104-115

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] In vivo dynamics of EBNA1-oriP interaction during latent and lytic replication of Epstein-Barr virus.2004

    • Author(s)
      Daikoku T, Kudoh A, Fujita M, Sugaya Y, Isomura H, Tsurumi T.
    • Journal Title

      Journal of Biological Chemistry. 24;279(52)

      Pages: 54817-54825

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Inhibition of S-phase cyclin-dependent kinase activity blocks expression of Epstein-Barr virus immediate-early and early genes, preventing viral lytic replication.2004

    • Author(s)
      Kudoh A, Daikoku T, Sugaya Y, Isomura H, Fujita M, Kiyono T, Nishiyama Y, Tsurumi T.
    • Journal Title

      Journal of Virology. 78(1)

      Pages: 104-115

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Epstein-Barr virus lytic replication elicits ATM checkpoint signal transduction while providing an S-phase-like cellular environment.2004

    • Author(s)
      Kudoh A, Fujita M, Zhang L, Shirata N, Daikoku T, Sugaya Y, Isomura H, Nishiyama Y, Tsurumi T
    • Journal Title

      Journal of Biological Chemistry 280(9)

      Pages: 8156-8163

    • Related Report
      2004 Annual Research Report
  • [Journal Article] In vivo dynamics of EBNA1-oriP interaction during latent and lytic replication of Epstein-Barr virus.2004

    • Author(s)
      Daikoku T, Kudoh A, Fujita M, Sugaya Y, Isomura H, Tsurumi T.
    • Journal Title

      Journal of Biological Chemistry 279(52)

      Pages: 54817-54825

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Inhibition of S-phase cyclin-dependent kinase activity blocks expression of Epstein-Barr virus immediate-early and early genes, preventing viral lytic replication.2004

    • Author(s)
      Kudoh A, Daikoku T.Sugaya Y, Isomura H, Fujita M, Kiyono T, Nishiyama Y, Tsurumi
    • Journal Title

      Journal of Virology 78(1)

      Pages: 104-115

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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