In vivo dynamics of EBNA1-oriP interaction during latent and lytic replication of Epstein-Barr virus.
| Project/Area Number |
16590398
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | University of Toyama (2006)
Aichi Cancer Center Research Institute (2004-2005) |
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Principal Investigator |
DAIKOKU Tohru University of Toyama, aboratory of Virology, Faculty of Medicine, Associate Professor, 医学薬学研究部ウイルス学, 助教授 (80291409)
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| Co-Investigator(Kenkyū-buntansha) |
菅谷 豊 愛知県がんセンター(研究所), リサーチレジデント
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | EBV / EBNA1 / ChIP / Replication compartments / ミスマッチ修復 |
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| Research Abstract |
The Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) is required for maintenance of the viral genome DNA during the latent phase of EBV replication but continues to be synthesized after the induction of viral productive replication. An EBV genome-wide chromatin immunoprecipitation assay revealed that EBNA1 constantly binds to oriP of the EBV genome during not only latent but also lytic infection. Although the total levels of EBNA1 proved constant throughout the latter, the levels of the oriP-bound form were increased as lytic infection proceeded. EBV productive DNA replication occurs at discrete sites in nuclei, called replication compartments, where viral replication proteins are clustered. Confocal laser microscopic analyses revealed that whereas EBNA1 was distributed broadly in nuclei as fine punctate dots during the latent phase of infection, the protein became redistributed to the viral replication compartments and localized as distinct spots within and/or nearby the compartments after the induction of lytic replication. Taking these findings into consideration, oriP regions of the EBV genome might be organized by EBNA1 into replication domains that may set up scaffolding for lytic replication and transcription.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] Establishment of a novel foreign gene delivery system combining an HSV amplicon with an attenuated replication-competent virus, HSV-1 HF10.2006
Author(s)
Zhang L, Daikoku T, Ohtake K, Ohtsuka J, Nawa A, Kudoh A, Iwahori S, Isomura H, Nishiyama Y, Tsurumi T.
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Journal Title
Journal of Virological Methods. 137(2)
Pages: 177-1
Description
「研究成果報告書概要(欧文)」より
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