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Genetic typing and clinical method of detecting metallo-beta-lactamase-producers in gram-negative rods in Japan

Research Project

Project/Area Number 16590470
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionNational Institute of Infectious Diseases, Japan

Principal Investigator

SHIBATA Naohiro  National Institute of Infectious Diseases, Japan, Bacterial Infection Control and Pathogenesis, chief researcher, 細菌第二部, 研究員 (50311402)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsmetallo-beta-lactamase / genetic typing / drug-resistance / gram-negative bacteria / 検出法 / メトロ-β-ラクタマーゼ
Research Abstract

Since metallo-beta-lactamase (MBLs) can hydrolyze a very wide range of broad-spectrum beta-lactams, MBL-producing gram-negative bacteria usually demonstrate consistent resistance to a variety of broad-spectrum beta-lactams, including oxyiminocephalosporins, cephamycins, and carbapenems, which are the least resort for control of infections caused by gram-negative bacteria. Thus, MBL-producing gram-negative bacteria have been recognized to be among the most important nosocomial pathogens, and further proliferation of these strains in clinical settings will pose a serious global problem in the future. The worldwide spread of this kind of organism is becoming a general concern, since several MBL-producing gram-negative bacteria have recently been reported outside Japan. On the other hand, the emergence of gram-negative bacterial species with acquired resistance to various broad-spectrum beta-lactams is becoming a worldwide clinical problem. Srains producing TEM- or SHV-derived extended-spe … More ctrum beta-lactamases (ESBLs) usually demonstrate high-level resistance to broad-spectrum oxyimino beta-lactams such as ceftiazidime and cefotaxime. Moreover, several K.pneumoniae strains that showed resistance to cephamycins as well as oxyimino cephalosporins were also found to priduce AmpC-type beta-lactamases. Indeed, PCR analyses usually give reliable and satisfactory results, but this method is of limited practical use for daily application in clinical laboratories because of the cost. We demonstrated a convenient test (SMA test) by using thiol compound in 2000. But this method was only MBL detection. In this study, we tried to screen beta-lactamase producers by using disk diffusion test with the combination of SMA and various antibiotic disks. By this method, CAZ-resistant strains producing AmpC or ESBLs were distinguishable from MBL producers. Therefore the method described in this study is very helpful for screening MBL- and other beta-lactamase-producing strains in dauly clinical labolatory testing. Less

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (18 results)

All 2006 2005 2004 2003

All Journal Article (18 results)

  • [Journal Article] PCR classification of CTX-M-type β-lactamase genes identified in clinically isolated gram-negative bacilli in Japan.2006

    • Author(s)
      Shibata N, H Kurokawa, Y Doi, et al.
    • Journal Title

      Antimicrob. Agents Chemoter. 50(2)

      Pages: 791-795

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Horizontal transfer of blaCMY-bearing plasmids among clinical Esherichia coil and Kiebsiella pneumoniae isolates and emergence of cefepime-hydroyzing CMY-19.2006

    • Author(s)
      Wachino J, H Kurokawa, S Suzuki, K Yamane, N Shibata, K Kimura, Y Ike, Y Arakawa
    • Journal Title

      Antimicrob. Agents Chemoter. 50(2)

      Pages: 534-541

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Novel plasmid-mediated 16S rRNA methirase, RmtC, found in a proteus mirabilis isolate demonstrating extraordinary high-lebel resistance against various aminoglicosides.2006

    • Author(s)
      Wachino J, K Yamane, S Suzuki, N Shibata, K Kimura, Y Arakawa
    • Journal Title

      Antimicrob. Agents Chemoter. 50(1)

      Pages: 178-184

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] PCR Classification of CTX-M-Type Beta-Lactamase Genes Identified in Clinically Isolated Gram-Negative Bacilli in Japan.2006

    • Author(s)
      Shibata N, H Kurokawa, Y Doi, T Yagi, K Yamane, J Wachino, S Satowa, K Kimura, S Ishikawa, H Kato, Y Ozawa, K Shibayama, K Kai, F Konda, Y Arakawa.
    • Journal Title

      Antimicrobial Agents and Chemotherapy 50(2)

      Pages: 791-795

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Horizontal transfer of blaCMY-bearing plasmids among clinical Esherichia coli and Klebsiella phew noniae isolates and emergence of cefepime hydroyzing CMY-19.2006

    • Author(s)
      Wachino J, H Kurokawa, S Suzuki, K Yamane, N Shibata, K Ki mura, Y Ike, Y Arakawa.
    • Journal Title

      Antimicrobial Agents and Chemotherapy 50(2)

      Pages: 534-541

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Novel plasmid-Mediated 16S rRNA Methilase, RmtC, found in a p roteus mirabilis isolate demonstrating extraordinary high-level resistance against various aminoglycosides.2006

    • Author(s)
      Wachino J, K Yamane, K Shibayama, H Kurokawa, N Shibata, S Suzuki, Y Doi, K Kimura, Y Ike, Y Arakawa
    • Journal Title

      Antimicrobial Agents and Chemotherapy 50(1)

      Pages: 178-184

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] PCR classification of CTX-M-type β-lactamase genes identified in clinically isolated gram-negative bacilli in Japan.2006

    • Author(s)
      Shibata N, H Kurokawa, Y Doi, et al.
    • Journal Title

      Antimicrob.Agents Chemoter. 50(2)

      Pages: 791-795

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Horizontal transfer of blaCMY-bearing plasmids among clinical Esherichia coli and Klebsiella pneumoniae isolates and emergence of cefepime-hydroyzing CMY-19.2006

    • Author(s)
      Wachino J, H Kurokawa, S Suzuki, K Yamane, N Shibata, K Kimura, Y Ike, Y Arakawa
    • Journal Title

      Antimicrob.Agents Chemoter. 50(2)

      Pages: 534-541

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Novel plasmid-mediated 16S rRNA methirase, RmtC, found in a proteus mirabilis isolate demonstrating extraordinary high-lebel resistance against various aminoglicosides.2006

    • Author(s)
      Wachino J, K Yamane, S Suzuki, N Shibata, K Kimura, Y Ike, Y Arakawa
    • Journal Title

      Antimicrob.Agents Chemoter. 50(1)

      Pages: 178-184

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Practical methods using boronic acid compounds for identification of class C beta-lactamases-producing Klebsiella pneumoniae and Esherichia coli.2005

    • Author(s)
      Yago T, J Wachino, K Yamane, S Suzuki, K Doi, N Shibata, et al.
    • Journal Title

      J Chin. Microbiology 43(6)

      Pages: 2551-2558

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Practical Methods Using Boronbic acid Compounds for Identification of Class C beta Lactamase-Producing Klebsiella pneumoniae and Esherichia coli.2005

    • Author(s)
      Yagi T, J Wachino, H Kurokawa, S Suzuki, K Yamane, Y Doi, N Shibata, H Kato, K Shibayama, Y Arakawa
    • Journal Title

      Journal of Clinical Microbiology 43(6)

      Pages: 2551-2558

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Practical methods using boronic acid compounds for identification of class C beta-lactamases-producing Klebsiella pneumoniae and Esherichia coli.2005

    • Author(s)
      Yago T, J Wachino, K Yamane, S Suzuki, K Doi, N Shibata, et al.
    • Journal Title

      J Clin.Microbiology 43(6)

      Pages: 2551-2558

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Esherichia coli Producing CTX M-2 Beta Lactamase in Cattle, Japan.2004

    • Author(s)
      Shiraki Y, N Shibata, Y Doi, Y Arakawa.
    • Journal Title

      Emerg Infect Dis. 10(1)

      Pages: 69-75

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Metallo-beta-Lactamase-Producing Gram Negativer Bacilli : Labolatory-Based Surveillance in Cooperation with 13 Clinical Labolatories in the Kinki Region of Japan.2004

    • Author(s)
      Hisaaki Nisio, Masaru Komatsu, Naohiro Shibata, Kouichi Shimakawa, Noriyuki Sueyoshi, Toshiro Orita, Tomomi Kofuku, Katsutoshi Yamasaki, Masako Sakamoto, Shohiro Kinoshita, Masanori Aihara, Yoshichika Arakawa
    • Journal Title

      Journal of Clinical Microbiology 42巻11号

      Pages: 5256-5263

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Eshericchia toll Producing CTX-M-2 beta Lactamase in Cattle, Japan.2004

    • Author(s)
      Yutaka Shiraki, Naohiro Shibata, Yohei Doi, Yoshihika Arakawa
    • Journal Title

      Emerging Infectious Diseases 10巻1号

      Pages: 69-75

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Nosocomial Transmission of CTX-M-2 β-Lactamase-Producing Acinetobacter baumannii in a Neurosurgery Ward.2004

    • Author(s)
      Noriyuki Nagano, Yukiko Nagano, Cristophie Cordevant, Naohiro Shibata, Yoshichika Arakawa
    • Journal Title

      Journal of Clinical Microbiology 42巻9号

      Pages: 3978-3984

    • Related Report
      2004 Annual Research Report
  • [Journal Article] PCR Typing of genetic Determinats for Metallo-Beta-Lactamases and Integrases Carried By Gram-Negative Bacteria Isolated In Japan, with Focus on the Class 3 Integron.2003

    • Author(s)
      Shibata N, Y Doi, K Yamane, T Yagi, H Kurokawa, K Shibayama, H Kato, K Kai, Y Arakawa.
    • Journal Title

      Journal of Clinical Microbiology 41(12)

      Pages: 5407-5413

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A New TEM-derived extended-spectrum beta-lactamase (TEM-91) with an R164C substitution at the omega-loop confers ceftazidime resistance.2003

    • Author(s)
      Kurokawa H, N Shibata, Y Doi, K Shibayama, K Kamachi, T Yagi, Y Arakawa.
    • Journal Title

      Antimicrobial Agents and Chemotherapy 47(9)

      Pages: 2981-2983

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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