Genetic typing and clinical method of detecting metallo-beta-lactamase-producers in gram-negative rods in Japan
| Project/Area Number |
16590470
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | National Institute of Infectious Diseases, Japan |
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Principal Investigator |
SHIBATA Naohiro National Institute of Infectious Diseases, Japan, Bacterial Infection Control and Pathogenesis, chief researcher, 細菌第二部, 研究員 (50311402)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | metallo-beta-lactamase / genetic typing / drug-resistance / gram-negative bacteria / 検出法 / メトロ-β-ラクタマーゼ |
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| Research Abstract |
Since metallo-beta-lactamase (MBLs) can hydrolyze a very wide range of broad-spectrum beta-lactams, MBL-producing gram-negative bacteria usually demonstrate consistent resistance to a variety of broad-spectrum beta-lactams, including oxyiminocephalosporins, cephamycins, and carbapenems, which are the least resort for control of infections caused by gram-negative bacteria. Thus, MBL-producing gram-negative bacteria have been recognized to be among the most important nosocomial pathogens, and further proliferation of these strains in clinical settings will pose a serious global problem in the future. The worldwide spread of this kind of organism is becoming a general concern, since several MBL-producing gram-negative bacteria have recently been reported outside Japan. On the other hand, the emergence of gram-negative bacterial species with acquired resistance to various broad-spectrum beta-lactams is becoming a worldwide clinical problem. Srains producing TEM- or SHV-derived extended-spe
… More
ctrum beta-lactamases (ESBLs) usually demonstrate high-level resistance to broad-spectrum oxyimino beta-lactams such as ceftiazidime and cefotaxime. Moreover, several K.pneumoniae strains that showed resistance to cephamycins as well as oxyimino cephalosporins were also found to priduce AmpC-type beta-lactamases. Indeed, PCR analyses usually give reliable and satisfactory results, but this method is of limited practical use for daily application in clinical laboratories because of the cost. We demonstrated a convenient test (SMA test) by using thiol compound in 2000. But this method was only MBL detection. In this study, we tried to screen beta-lactamase producers by using disk diffusion test with the combination of SMA and various antibiotic disks. By this method, CAZ-resistant strains producing AmpC or ESBLs were distinguishable from MBL producers. Therefore the method described in this study is very helpful for screening MBL- and other beta-lactamase-producing strains in dauly clinical labolatory testing. Less
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Report
(3 results)
Research Products
(18 results)
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[Journal Article] PCR Classification of CTX-M-Type Beta-Lactamase Genes Identified in Clinically Isolated Gram-Negative Bacilli in Japan.2006
Author(s)
Shibata N, H Kurokawa, Y Doi, T Yagi, K Yamane, J Wachino, S Satowa, K Kimura, S Ishikawa, H Kato, Y Ozawa, K Shibayama, K Kai, F Konda, Y Arakawa.
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Journal Title
Antimicrobial Agents and Chemotherapy 50(2)
Pages: 791-795
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Novel plasmid-Mediated 16S rRNA Methilase, RmtC, found in a p roteus mirabilis isolate demonstrating extraordinary high-level resistance against various aminoglycosides.2006
Author(s)
Wachino J, K Yamane, K Shibayama, H Kurokawa, N Shibata, S Suzuki, Y Doi, K Kimura, Y Ike, Y Arakawa
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Journal Title
Antimicrobial Agents and Chemotherapy 50(1)
Pages: 178-184
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Metallo-beta-Lactamase-Producing Gram Negativer Bacilli : Labolatory-Based Surveillance in Cooperation with 13 Clinical Labolatories in the Kinki Region of Japan.2004
Author(s)
Hisaaki Nisio, Masaru Komatsu, Naohiro Shibata, Kouichi Shimakawa, Noriyuki Sueyoshi, Toshiro Orita, Tomomi Kofuku, Katsutoshi Yamasaki, Masako Sakamoto, Shohiro Kinoshita, Masanori Aihara, Yoshichika Arakawa
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Journal Title
Journal of Clinical Microbiology 42巻11号
Pages: 5256-5263
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