| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
|
|---|
| Research Abstract |
Since metallo-beta-lactamase (MBLs) can hydrolyze a very wide range of broad-spectrum beta-lactams, MBL-producing gram-negative bacteria usually demonstrate consistent resistance to a variety of broad-spectrum beta-lactams, including oxyiminocephalosporins, cephamycins, and carbapenems, which are the least resort for control of infections caused by gram-negative bacteria. Thus, MBL-producing gram-negative bacteria have been recognized to be among the most important nosocomial pathogens, and further proliferation of these strains in clinical settings will pose a serious global problem in the future. The worldwide spread of this kind of organism is becoming a general concern, since several MBL-producing gram-negative bacteria have recently been reported outside Japan. On the other hand, the emergence of gram-negative bacterial species with acquired resistance to various broad-spectrum beta-lactams is becoming a worldwide clinical problem. Srains producing TEM- or SHV-derived extended-spe
… More
ctrum beta-lactamases (ESBLs) usually demonstrate high-level resistance to broad-spectrum oxyimino beta-lactams such as ceftiazidime and cefotaxime. Moreover, several K.pneumoniae strains that showed resistance to cephamycins as well as oxyimino cephalosporins were also found to priduce AmpC-type beta-lactamases. Indeed, PCR analyses usually give reliable and satisfactory results, but this method is of limited practical use for daily application in clinical laboratories because of the cost. We demonstrated a convenient test (SMA test) by using thiol compound in 2000. But this method was only MBL detection. In this study, we tried to screen beta-lactamase producers by using disk diffusion test with the combination of SMA and various antibiotic disks. By this method, CAZ-resistant strains producing AmpC or ESBLs were distinguishable from MBL producers. Therefore the method described in this study is very helpful for screening MBL- and other beta-lactamase-producing strains in dauly clinical labolatory testing. Less
|
