Shift of dominant genetic clusters of Japanese encephalitis virus in Kyushu areas.
| Project/Area Number |
16590477
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Hygiene
|
|---|
| Research Institution | Oita University |
|---|
Principal Investigator |
MAKINO Yoshihiro Oita University, Faculty of Medicine, Professor, 医学部, 教授 (60039930)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MA Shao-Ping Oita University, Faculty of Medicine, Research fellow, 医学部, 助手 (50301396)
|
|---|
| Project Period (FY) |
2004 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
|
|---|
| Keywords | Japanese encephalitis virus / Genotype / Phylogenetic tree / Neutralization test / 日本脳炎ウィルス / 遺伝子系統樹 / 遺伝子抗体現象 / 遺伝型の交代現象 |
|---|
| Research Abstract |
Japanese encephalitis virus (JEV)
Japanese encephalitis virus (JEV) has been classified into 4 subtypes (G1-4) based on the molecular phylogenetic analysis of the C-prM gene region (240 base) and the location of isolation. The subtype of Japanese strains was believed to be G3. But, we found that since around 1994, the dominant strain had shifted from G3 to G1, which belonged to the northern Thailand subgroup. It is suggested that these G1 strains might be introduced into Japan from abroad. Phylogenetic analyses based on the C-prM gene region of 23 JEV strains isolated at Nagasaki, Okinawa, Osaka, Ishikawa and Tokyo during 1965-2001 indicated that G3 strains were further divided into 6 clusters (A, B, C, D, E1 and E2), each one of which became dominant during 1935-1994. Antigenic difference among the strains belong to different genetic clusters was examined by 50% plaque-reduction neutralization (NT) test. Six JEV strains and 20 swine sera naturally infected with JEV were used. The results indicated that NT reactivity showed significant difference (P<0.05) (1)between the JEV strains isolated in 1981 and 1989 ; (2)between the strains isolated in 1989 and 1995 ; and (3)between the two strains isolated in 1995. However, NT reactivity was not significantly different between strains isolated in 1981 and 1995. The results indicate that there is a possibility of introduction of antigenically distinct JEV strains into Japan. Continuous JEV surveillance is necessary.
|
Report
(3 results)
Research Products
(2 results)
-
-
[Journal Article] Shift in Japanese encephalitis virus (JEV) genotype circulating in northern Vietnam : implications for frequent introductions of JEV from Southeast Asia to East Asia.2004
Author(s)
Nga, PT, Parquet MC, Cuong VD, Ma S-P, Hasebe F., Inoue S., Makino Y., Takagi M, Nam VS, Marita K.
-
Journal Title
Journal of General Virology 85
Pages: 1625-1631
Description
「研究成果報告書概要(欧文)」より
Related Report
