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The large-scale gene analysis to develop the tailor-made prevention of osteoporosis

Research Project

Project/Area Number 16590522
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Public health/Health science
Research InstitutionKinki University

Principal Investigator

MORITA Akemi  Kinki University, School of Medicine, Department of Public Health, Assistant Professor, 医学部, 講師 (40262638)

Co-Investigator(Kenkyū-buntansha) IKEDA Yukihiro  Kinki University, School of Medicine, Department of Public Health, Assistant Researcher, 医学部, 助手 (20368294)
DOHI Yoshiko  Nara Medical University, Department of Public Health, Associate Professor, 医学部, 助教授 (50155628)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsOsteoporosis / Polymorphism / Randomized Cohort / Bone Mineral Density / Life-style / Tailor-maid Prevention / VDR遺伝子 / TGF-β遺伝子
Research Abstract

Using a large-scale representative sample of the Japanese female population, we examined the effects of single nucleotide polymorphisms in the VDR, TGF-β, Osteocalcin, MTHFR, CYP19, CYP1A1 and COMT gene on bone mineral density (BMD), and the interactions between these polymorphisms and lifestyle factors on BMD. Fifty women were randomly selected from each of the 5-year age-stratified populations (15-79 years) in each of three chosen municipalities as a part of the Japanese population-based osteoporosis (JPOS) baseline study. BMD at the lumbar spine, hip and distal forearm were measured using DXA at baseline, and again in a follow-up study conducted three years after the baseline.
Information on lifestyle factors was collected in a questionnaire and followed up in interviews. The polymorphisms were determined by the PCR-RFLP method and a TaqMan allelic discrimination assay. At baseline, 1,340 women were analyzed. There was no association between each genotype and the baseline BMD or the change in BMD at any of the skeletal sites. We found significant associations between daily milk consumption and baseline BMD at some skeletal sites, but only in subjects with GG genotype within a binding site of Cdx-2 in the VDR gene. There were significant positive associations between the natto intake and the rates of changes in BMD in postmenopausal women of JPOS study. We found significant interactions between the natto intake and CYP19 and COMT polymorphisms on BMD at femoral neck in postmenopausal women. In addition, the prevalence of osteoporosis and osteopenia was different between the genotypes of CYP19.
In conclusion, each polymorphism alone did not have substantial effect on BMD in Japanese women. However, these polymorphisms might have some effect in women with specific lifestyle factors. Currently, we are studying the effects of other candidate genes on BMD and searching new polymorphisms associated with bone formation.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (30 results)

All 2006 2005 2004

All Journal Article (28 results) Book (2 results)

  • [Journal Article] Intake of fermented soybeans, natto, is associated with reduced bone loss in postmenopausal women : -Japanese Population-based Osteoporosis (JPOS) Study-2006

    • Author(s)
      Yukihiro Ikeda
    • Journal Title

      Journal of Nutrition accepted

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Intake of fermented soybeans, natto, is associated with reduced bone loss in postmenopausal women : -Japanese Population-based Osteoporosis (JPOS) Study-2006

    • Author(s)
      Yukihiro Ikeda
    • Journal Title

      Journal of Nutrition (accepted)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Intake of fermented soybeans, natto, is associated with reduced bone loss in postmenopausal women : - Japanese Population-based Osteoporosis (JPOS) Study-2006

    • Author(s)
      Yukihiro Ikeda
    • Journal Title

      Journal of Nutrition accepted

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The effects of the Cdx-2 polymorphism in the promoter region of the vitamin D receptor gene and its interactions with lifestyle factors on bone mineral density in representative sample of Japanese women-the Japanese Population-based Osteoporosis (JPOS) Study2005

    • Author(s)
      Akemi Morita
    • Journal Title

      Calcified Tissue International Vol. 77(6)

      Pages: 339-347

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 骨折・骨組鴛症の遺伝要因とその予防医学的利用2005

    • Author(s)
      森田明美
    • Journal Title

      Clinical Calcium Vol. 15(8)

      Pages: 1364-1371

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Role of metallothionein isoforms in bone formation processes in rat marrow mesenchymal stem cells in culture2005

    • Author(s)
      Yoshiko Dohi
    • Journal Title

      Biological Trace Element Research Vol. 104 (1)

      Pages: 57-70

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The effects of the Cdx-2 polymorphism in the promoter region of the vitamin D receptor gene and its interactions with lifestyle factors on bone mineral density in representative sample of Japanese women-the Japanese Population-based Osteoporosis (JPOS) Study2005

    • Author(s)
      Akemi Morita
    • Journal Title

      Calcif.Tissue Int. 77(6)

      Pages: 339-347

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Genetic factors of osteoporosis and fracture and the application of genetic information to the preventive medicine2005

    • Author(s)
      Akemi Morita
    • Journal Title

      Clin Calcium. 15(8)

      Pages: 1364-1371

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Role of metallothionein isoforms in bone formation processes in rat marrow mesenchymal stem cells in culture2005

    • Author(s)
      Yoshiko Dohi
    • Journal Title

      Biol.Trace Elem.Res. 104(1)

      Pages: 57-70

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The effects of the Cdx-2 polymorphism in the promoter region of the vitamin D receptor gene and its interactions with lifestyle factors on bone mineral density in representative sample of Japanese women-the Japanese Population-based Osteoporosis (JPOS) Study2005

    • Author(s)
      Akemi Morita
    • Journal Title

      Calcified Tissue International Vol.77(6)

      Pages: 339-347

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 骨折・骨粗鬆症の遺伝要因とその予防医学的利用2005

    • Author(s)
      森田明美
    • Journal Title

      Clinical Calcium Vol.15(8)

      Pages: 1364-1371

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Role of metallothionein isoforms in bone formation processes in rat marrow mesenchymal stem cells in culture2005

    • Author(s)
      Yoshiko Dohi
    • Journal Title

      Biological Trace Element Research Vol.104(1)

      Pages: 57-70

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Precision control and seasonal variations in quantitative ultrasound measurement of the calcaneus2004

    • Author(s)
      Yukihiro Ikeda
    • Journal Title

      Journal of Bone and Mineral Metabolism Vol. 22(6)

      Pages: 588-593

    • NAID

      10014316820

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Prediction of bone mineral density from vitamin D receptor polymorphisms is uncertain in representative samples of Japanese women. The Japanese Population-based Osteoporosis (JPOS)Study2004

    • Author(s)
      Akemi Morita
    • Journal Title

      International Journal of Epidemiology Vol. 33(5)

      Pages: 979-988

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 超音波による骨量測定2004

    • Author(s)
      池田行宏
    • Journal Title

      総合臨床 Vol. 53(9)

      Pages: 2553-2554

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of the cDNA encoding bullfrog, Rana catesbeiana, osteocalcin and two forms of the protein isolated from bone2004

    • Author(s)
      Yoshiko Dohi
    • Journal Title

      Biochimie Vol. 86(7)

      Pages: 471-480

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 骨の再生医学(特集 : 骨・軟骨破壊の分子機構から修復再生に向けて)2004

    • Author(s)
      土肥祥子
    • Journal Title

      分子リウマチ Vol. 1(2)

      Pages: 99-106

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Ultrasound bone densitometry of the calcaneus with Sahara in healthy Japanese adolescents - Japanese Population-based Osteoporosis (JPOS) study -2004

    • Author(s)
      Yukihiro Ikeda
    • Journal Title

      Journal of Bone and Mineral Metabolism Vol. 22(3)

      Pages: 248-253

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] ビタミンD受容体(VDR)遺伝子多型2004

    • Author(s)
      森田明美
    • Journal Title

      日本臨床 Vol. 62 (Suppl 2)

      Pages: 160-163

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Precision control and seasonal variations in quantitative ultrasound measurement of the calcaneus2004

    • Author(s)
      Yukihiro Ikeda
    • Journal Title

      Journal of Bone and Mineral Metabolism. 22(6)

      Pages: 588-593

    • NAID

      10014316820

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Prediction of bone mineral density from vitamin D receptor polymorphisms is uncertain in representative samples of Japanese women. The Japanese Population-based Osteoporosis (JPOS) Study2004

    • Author(s)
      Akemi Morita
    • Journal Title

      Int.J.Epidemiol. 33(5)

      Pages: 979-988

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Bone mass measurement by means of quantitative ultrasound2004

    • Author(s)
      Yukihiro Ikeda
    • Journal Title

      Sogo Rinsho. 53(9)

      Pages: 2553-2554

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of the cDNA encoding bullfrog, Rana catesbeiana, osteocalcin and two forms of the protein isolated from bone2004

    • Author(s)
      Yoshiko Dohi
    • Journal Title

      Biochimie. 86(7)

      Pages: 471-480

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Regenerative medicine of bone2004

    • Author(s)
      Yoshiko Dohi
    • Journal Title

      Molecular Rheumatology. 1(2)

      Pages: 99-106

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Ultrasound bone densitometry of the calcaneus with Sahara in healthy Japanese adolescents - Japanese Population-based Osteoporosis (JPOS) study -2004

    • Author(s)
      Yukihiro Ikeda
    • Journal Title

      Journal of Bone and Mineral Metabolism. 22(3)

      Pages: 248-253

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Association of vitamin D receptor gene polymorphisms with osteoporosis2004

    • Author(s)
      Akemi Morita
    • Journal Title

      Nippon Rinsho. 62 Suppl 2

      Pages: 160-163

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Prediction of bone mineral density from vitamin D receptor polymorphisms is uncertain in representative samples of Japanese women. The Japanese Population-based Osteoporosis (JPOS) Study2004

    • Author(s)
      Akemi Morita
    • Journal Title

      Int J Epidemiol Vol.33(5)

      Pages: 979-988

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Ultrasound bone densitometry of the calcaneus with Sahara in healthy Japanese adolescents : Japanese Population -based Osteoporosis (JPOS) study2004

    • Author(s)
      Yukihiro Ikeda
    • Journal Title

      Journal of Bone and Mineral Metabolism Vol.22(3)

      Pages: 248-253

    • Related Report
      2004 Annual Research Report
  • [Book] 地域におけるエビデンスに基づく骨折・骨粗鬆症予防ガイドライン2004

    • Author(s)
      伊木雅之
    • Total Pages
      133
    • Publisher
      日本公衆衛生協会
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Book] Evidence-based guideline for prevention of osteoporosis and osteoporotic fractures in community health2004

    • Author(s)
      Masayuki Iki, editor
    • Total Pages
      113
    • Publisher
      Japan Public Health Association
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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