| Project/Area Number |
16590589
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | KINKI UNIVERSITY (2005)
Kyoto University (2004) |
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Principal Investigator |
NABESHIMA Motoshige (2005) KINKI UNIVERSITY, Hospital, Associate professor, 医学部附属病院, 助教授 (80314178)
妹尾 浩 (2004) 京都大学, 医学研究科, 助手 (90335266)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAMOTO Shinichi Kyoto University, Department of Gastroenterology and Hepatology, Assistant professor, 医学研究科, 助手 (90378761)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | colon / β-catenin / inflammation / tumorigenesis / stroma / IL-6 / Gas6 / cytokine / β一カテニン / 上皮・間質相互作用 / Th-1 / COX-2 |
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| Research Abstract |
To investigate the role of stromal factors during colorectal tumorigenesis, we determined the factors highly expressed in cancer stroma using laser capture microdissection system and DNA arrays. Among those factors, we analyzed the effects of IL-6, COX-2 and Gas6 in this study.
First, in SW480 cells we observed translocation of β-catenin from the nucleus to the cytoplasm by inhibition of STAT3 which transmits IL-6 signaling. Second, by using co-culture system we demonstrated that local hypoxia up-regulated COX-2 expression in tumor stromal cells. Third, we found that Gas6 activated PI3K/Akt pathway in DLD-1 cells with suppression of cellular apoptosis.
These data suggest a strong association between stromal factors and cancer cell behaviors, and may lead us to novel therapeutic strategies.
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