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Development of New device for Regeneration of small intestinal tissue

Research Project

Project/Area Number 16590590
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionKyoto University

Principal Investigator

NAKASE Hiroshi  Kyoto University, Department of Gastroenterology & Hepatology, Assistant Professor, 医学研究科, 講師 (60362498)

Co-Investigator(Kenkyū-buntansha) TABATA Yasuhiko  Kyoto University, Institute for Frontier Medical Science, Professor, 再生医科学研究所, 教授 (50211371)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordssmall intestine regenerating tube / bone marrow cell / basic FGF / bFGF / basic FGF
Research Abstract

1. Development of innovative polylactic acid tube (or polyglycolic acid) for regenerating small intestinal tissue. We have completed innovative polylactic acid (or polyglycolic acid) tube which could be suitable for regeneration of small intestine. We also showed that bFGF is most useful growth factor for regeneration of small intestinal tissue. Therefore, we completed the biodegradable gelatin microspheres, which can continuously release bFGF. We attached these microspheres to inside of the tube and injected bone marrow cells into this tube (we named it as small intestinal regenerating tube=SIR tube.). We transplanted SIR tube to small intestine of rat, which had taken resection of small intestine for experiment. Interestingly, regeneration of small intestinal tissue was observed inside of SIR tube 2 months after transplanting.
2. We checked regenerated small intestinal tissue histologically and functionally. As a result, minute endoscopic findings revealed that villous epithelium was appeared one month after transplanting.
3. Moreover, we investigated the proportion of immune-regulating cells in regenerated small intestine. Histological and FACS analysis revealed that there was no significant difference of the proportion of macrophages, T, and B cells in between regenerated and normal small intestine.
4. We injected GFP bone marrow cells in SIR tube. Three months after transplanting this tube, we investigated what kinds of cell GFP bone marrow cells would differentiate into in regenerated small intestine. As a result, confocal microscopic findings showed that GFP was positive for myofibroblasts and small intestinal epithelium, which suggested that transplanted bone marrow cells differentiated into epithelium and mesenchymal cells and compose of newly regenerated small intestine.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (24 results)

All 2006 2005 2004 Other

All Journal Article (23 results) Book (1 results)

  • [Journal Article] Specific Antibodies Against Recombinant Protein of Insertion element 900 of Mycobacterium avium subspecies paratuberculosis in Japanese patients with Crohn's Disease.2006

    • Author(s)
      Nakase H, et al.
    • Journal Title

      Inflammatory Bowel Disease 12

      Pages: 62-69

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Solitary rectal ulcer syndrome with ulcerative colitis.2006

    • Author(s)
      Uza N, Nakase H, et al.
    • Journal Title

      Gastrointestinal Endoscopy 63

      Pages: 355-356

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Protective role of redox-active thioredoxin-1 for severe acute pancreatitis2006

    • Author(s)
      Ohashi S, Nakase H, et al.
    • Journal Title

      Am J Physiol Gastrointest Liver Physiol 290

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Gastric mucosal hyperplasia via up-regulation of gastrin induced by persistent activation of gastric innate immunity in MHC class II-deficient mice.2006

    • Author(s)
      Fukui T, Nakase H, et al.
    • Journal Title

      GUT 55

      Pages: 607-615

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Meckel's diverticulum with an enterolith.2006

    • Author(s)
      Uza N, Nakase H, et al.
    • Journal Title

      Gastroenterology (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Specific antibodies against recombinant proteins of insertion element 900 of mycobacterium avium subspecies paratuberculosis in Japanese patients with Crohn's Disease2006

    • Author(s)
      Nakase H, et al.
    • Journal Title

      Inflammatory bowel disease 12

      Pages: 62-69

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Solitary rectal ulcer syndrome with ulcerative colitis2006

    • Author(s)
      Uza N, Nakase H, et al.
    • Journal Title

      Gastrointestinal Endoscopy. 63

      Pages: 355-356

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Protective role ofredox-active thioredoxin-1 for severe acute pancreatitis2006

    • Author(s)
      Ohashi S, Nakase H, et al.
    • Journal Title

      Am J Physiol Gastrointest Liver Physiol 290

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Gastric mucosal hyperplasia via up-regulation of gastrin induced by persistent activation of gastric innate immunity in MHC class II-deficient mice.2006

    • Author(s)
      Fukui H, Nakase H, et al.
    • Journal Title

      Gut 55

      Pages: 607-615

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Rectal carcinoid tumor mimicking colonic adenomatous lesion2006

    • Author(s)
      Nakase H, et al.
    • Journal Title

      Gastrointestinal Endoscopy 62

      Pages: 976-977

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Specific Antibodies Against Recombinant Protein of Insertion element 900 of Mycobacterium avium subspecies paratuberculosis in Japanese patients with Crohn's Disease.2006

    • Author(s)
      Nakase H, et al.
    • Journal Title

      Inflamm Bowel Disease 12

      Pages: 62-69

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Solitary rectal ulcer syndrome with ulcerative colitis.2006

    • Author(s)
      Uza N, Nakase H, et al.
    • Journal Title

      Gastrointest Endosc 63

      Pages: 355-356

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Image of the Month : Meckel's diverticulum with an enterolith.2006

    • Author(s)
      Uza N, Nakase H, et al.
    • Journal Title

      Gastroenterology (印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Rectal Carcinoid Tumor Mimicking Colonic Adenomatous Lesion.2005

    • Author(s)
      Nakase H, et al.
    • Journal Title

      Gastrointestinal Endoscopy 62

      Pages: 976-977

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Rectal Carcinoid Tumor Mimicking Colonic Adenomatous Lesion.2005

    • Author(s)
      Nakase H, et al.
    • Journal Title

      Gastrointest Endosc 62

      Pages: 976-977

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Possible role of REG I{alpha} protein in ulcerative colitis and colitic cancer.2005

    • Author(s)
      Sekikawa A, Nakase H, et al.
    • Journal Title

      Gut 54

      Pages: 1437-1444

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Refractory enterovesical and duodenocolic fistulas in Crohn's disease successfully managed with tacrolimus.2005

    • Author(s)
      Fukuda A, Nakase H, et al.
    • Journal Title

      J Gastroenterol (印刷中)

    • NAID

      10015710243

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Therapeutic effects of rectal administration of basic fibroblast growth factor on experimental murine colitis.2005

    • Author(s)
      Matsuura M, Nakase H, et al.
    • Journal Title

      Gastroenterology (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Colonic ameboma2005

    • Author(s)
      Matsuura M, Nakase H, et al.
    • Journal Title

      Gastrointest Endosc (発表予定)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Characteristics of gastric B-cell lymphoma of mucosa-associated lymphoid tissue type involving multiple organs.2004

    • Author(s)
      Iwano M, Nakase H, et al.
    • Journal Title

      J Gastroenterol. 39

      Pages: 739-746

    • NAID

      80016835116

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Environmental pollutant tributyltin promotes Th2 polarization and exacerbates airway inflammation.2004

    • Author(s)
      Kato T, Nakase H, et al.
    • Journal Title

      Eur J Immunol. 34

      Pages: 1312-1321

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Metastatic thyroid lymphoma to gastroduodenum2004

    • Author(s)
      Katayama M, Nakase H, et al.
    • Journal Title

      Gastrointest Endosc 60

      Pages: 605-606

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Meckel's diverticulum with an enteolith

    • Author(s)
      Uza N, Nakase H, et al.
    • Journal Title

      Gastroenterology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 最新医学 特集 炎症性腸疾患-最近の知見と今後解決されるべき問題点-2004

    • Author(s)
      仲瀬裕志, 千葉 勉
    • Total Pages
      142
    • Publisher
      最新医学社
    • Related Report
      2004 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

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