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DNA methylation through prostaglandin in colon carcinogenesis

Research Project

Project/Area Number 16590626
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionSaitama Medical School

Principal Investigator

OTA Shinichi  Saitama Medical School, Medicine, Professor, 医学部, 教授 (30185269)

Co-Investigator(Kenkyū-buntansha) ARAI Shin  Saitama Medical School, Medicine, Assistant Professor, 医学部, 講師 (20306294)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordscolon cancer / prostaglandin / methylation / COX-2
Research Abstract

1.The effects of prostaglandin (PG) E_2 and PGJ_2 on gene expression were studied using cDNA microarray. Several genes were identified. Results of some genes were not compatible with that of PCR, and these discrepancies were under investigation.
2.Levels of P1GF protein were not affected by PG.
3.Among several cytokines, PGE_2 and PGJ_2 dramatically reduced TNF-α production by human macrophages.
4.COX-2, a prostaglandin synthetase, was expressed in subepithelial mesenchymal cells of early colon cancer, laterally spreading tumor, and colon adenoma.
5.COX-2 expressing cells were α-smooth actin negative and partly CD68 positive. Therefore, these cells were partly macrophages but not myofibroblasts.
6.To investigate the methylation of APC genes, expression of β-cathenin, which is inactivated by APC protein, was studied. Transfer of this protein from cytoplasm to nucleus was not observed in adenomas, but was found in colon cancer.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (9 results)

All 2006 2005 Other

All Journal Article (5 results) Book (4 results)

  • [Journal Article] Prostaglandins in gastrointestinal diseases2006

    • Author(s)
      Bamba H, Ota S
    • Journal Title

      Current Topics in Biochemical Research (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Prostaglandin in gastrointestinal diseases.2006

    • Author(s)
      Bamba H, Ota S.
    • Journal Title

      Current Topics in Biochemical Research (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Role of cyclooxygenase-2 in gastric carcinogenesis.2005

    • Author(s)
      Bamba H, Ota S., et al.
    • Journal Title

      6^<th> International Gastric cancer Congress (Editor : Kitajima M and Otani Y, Medimond S.r.l, Bologna)

      Pages: 295-299

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Chaptor VI : COX-2, prostaglandins, and colon cancer2005

    • Author(s)
      Ota S., Bamba H.
    • Journal Title

      Focus Colorectal Cancer Research (Editor : Julia D.Martinez)(Nova Science Publishers, Inc., New York)

      Pages: 123-141

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Prostaglandins in gastrointestinal diseases

    • Author(s)
      Ota s, Bamba H.
    • Journal Title

      Current Topics in Biochemical Research (in press)

    • Related Report
      2005 Annual Research Report
  • [Book] 6^<th> International Gastric Cancer Congress2005

    • Author(s)
      Bamba H, Ota S, et al.
    • Total Pages
      601
    • Publisher
      Medimond S. r. l
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] Focus in Colorectal Cancer Research2005

    • Author(s)
      Ota S, Bamaba H.
    • Total Pages
      322
    • Publisher
      Nova Science Publishers
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 6^<th> International Gastric Cancer Congress2005

    • Author(s)
      Bamba H, Ota S, et al.
    • Total Pages
      601
    • Publisher
      International Proceedings Division
    • Related Report
      2005 Annual Research Report
  • [Book] Focus in Colorectal Cancer Research2005

    • Author(s)
      Ota s, Bamba H.
    • Total Pages
      322
    • Publisher
      Nova Science Publishers
    • Related Report
      2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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