Analysis of replication of Hepatitis C Virus using highly efficient replication system
| Project/Area Number |
16590653
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Tokyo Metropolitan Organization for Medical Research |
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Principal Investigator |
WAKITA Takaji Tokyo Metropolitan Organization for Medical Research, Tokyo Metropolitan Institute for Neuroscience, Senior Research Scientist, 東京都神経科学総合研究所, 副参事研究員 (40280789)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAMOTO Michiko Tokyo Metropolitan Organization for Medical Research, Tokyo Metropolitan Institute for Neuroscience, Scientist, 研究員 (40190821)
KATO Takanobu Nagoya City University, Graduate School of Medicine, Assistant Professor, 大学院・医学研究科, 助手 (20333370)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | HCV / virus / viral genome replication / HCC / chronic hepatitis / interferon |
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| Research Abstract |
Hepatitis C virus (HCV) is a major public health problem, infecting an estimated 170 million people worldwide. Current therapy for HCV-related chronic hepatitis is based on the use of interferon. However, virus clearance rates are insufficient. Investigations to develop the anti-viral therapy or to understand the life cycle of this virus have been hampered by the lack of viral culture systems. We isolated the JFH-1 strain from a patient with fulminant hepatitis, and the JFH-1 subgenomic replicon could replicate efficiently in culture cell without adaptive mutation. Using this JFH-1 strain, we developed highly efficient HCV replication system in culture cells. The full-length JFH-1 RNA was transfected into Huh7 cells. Subsequently, viral RNA efficiently replicated in transfected cells and viral particles were secreted. Secreted viral particle exhibited spherical shape by electron-microscopic analysis and was separated in the fraction eith 1.15g/ml density. Furthermore, secreted virus displayed infectivity for naive Huh7 cells. In addition, we have also developed an replicon system using full-genomic JFH-1 genome. Both systems provide powerful tools for studying the viral life cycle and constructing anti-viral strategies.
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Report
(3 results)
Research Products
(40 results)
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[Journal Article] Subcellular localization of hepatitis C virus structural proteins in a cell culture system that efficiently replicates the virus.2006
Author(s)
Y Rouille, F Helle, D Deigrange, P Roingeard, C Voisset, E Blanchard, S Belouzard, J McKeating, A Patel, G Maertens, T Wakita, C Wychowski, J Dubuisson
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Journal Title
Journal of Virology (in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Detection of Anti-Hepatitis C Virus Effects of Interferon and Ribavirin by a Sensitive Replicon System2005
Author(s)
T Kato, T Date, M Miyamoto, M Sugiyama, Y Tanaka, E Orito, T Ohno, K Sugihara, I Hasegawa, K Fujiwara, K Ito, A Ozasa, M Mizokami, T Wakita.
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Journal Title
Journal of Clinical Microbiology 43
Pages: 5679-5684
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Production of Infectious Hepatitis C Virus in Tissue Culture from a Cloned Viral Genome2005
Author(s)
T Wakita, T Pietschmann, T Kato, T Date, M Miyamoto, Z Zhao, K Murthy, A Habermann, H-G Krausslich, M Mizokami, R Bartenschlager, T J Liang
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Journal Title
Nat Med. 11
Pages: 791-796
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Nuclear Localization of Japanese Encephalitis Virus Core Protein Enhances Viral Replication2005
Author(s)
Y Mori, T Okabayashi, T Yamashita, Z Zhao, T Wakita, K Yasui, F Hasebe, M Tadano, E Konishi, K Moriishi, Y Matsuura.
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Journal Title
J Virol. 79
Pages: 3448-3458
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Regulation of Hepatitis C Virus Replication by Interferon Regulatory Facctor-12004
Author(s)
Kanazawa N, Kurosaki M, Sakamoto N, Enomoto N, Itsui Y, Yamashiro T, Tanabe Y, Maekawa S, Nakagawa M, Chen C-H, Oshima S, Nakamura T, Kato T, Wakita T, Watanabe M
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Journal Title
J Virol 78
Pages: 9713-9720
Description
「研究成果報告書概要(欧文)」より
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