| Project/Area Number |
16590737
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YAMAMOTO Hiroshi The University of Tokyo, Faculty of Medicine, Assistant, 医学部附属病院, 助手 (10361487)
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| Co-Investigator(Kenkyū-buntansha) |
NAGASE Takahide The University of Tokyo, Faculty of Medicine, Professor, 医学部附属病院, 教授 (40208004)
SHINDO Takayuki Shinshu University, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (90345215)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | bronchial asthma / pulmonary fibrosis / adrenomedullin / 遺伝子改変マウス / 気道過敏症 |
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| Research Abstract |
Heterozygous Adrenomedullin (AM) gene deficient (AMKO) mice and their litter mate control (Wild type) were applied for this study. OVA-induced murine asthma model mice were introduced ; hence they showed greater responsiveness to Methacholine (MCh) inhalation challenge than the wild type (EC200RL : saline-treated AM^<+/+>, 16.81 ± 2.01mg/ml ; saline-treated AM^<+/->, 16.73± 2.34mg/ml ; OVA-treated AM^<+/+>, 7.95 ± 0.98mg/ml ; OVA-treated AM^<+/->, 2.41 ± 0.63^*mg/ml, respectively, ^*P<0.05 vs. the other groups). Immunoreactive AM in the lung tissue of AMKO mice before and after MCh challenge was significantly lower than the wild type. Tissue AM insufficiency might affect enhance airway responsiveness Morphometrical analysis of the sampled lung tissue sections revealed significant airway narrowing, owing to the trophic change of airway smooth muscle cells and/or the swelling of airway epithelial cells. Peribronchial infiltration by eosinophils, airway hypersecretion or goblet cell hyperplasia, TH1, Th2 cytokines, leukotrienes were assessed, but no significant difference between AMKO mice and wild type mice.
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