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Regulatory mechanisms for lung and airway fibrosis

Research Project

Project/Area Number 16590747
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionHiroshima University

Principal Investigator

YOKOYAMA Akihito  Hiroshima University, Graduate School of Biomedical Sciences, Associate Professor, 大学院・医歯薬学総合研究科, 助教授 (30191513)

Co-Investigator(Kenkyū-buntansha) KOHNO Nobuoki  Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (80215194)
近藤 圭一  島根大学, 医学部附属病院, 講師 (20332827)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsbronchial asthma / cytokine / fibroblast / T cells / interferon / pulmonary fibrosis / remodeling
Research Abstract

The purpose of this study is to disclose the mechanisms of airway and lung parenchymal fibrosis. We specifically explore the roles of T cells and a negative regulator of cytokine signaling, suppressor of cytokine signaling-1 (SOCS-1) in the regulatory mechanisms of lung fibrosis.
(1) By using polarized Th1 and Th2 cells from TCR-transgenic mice, we demonstrated that activation of Th1 cells followed by Th2 cells in the lung can induce lung fibrosis in vivo.
(2) To investigate the characteristics of fibroblasts in idiopathic pulmonary fibrosis (IPF), we obtained 8 fibroblast cell lines from lungs with IPF and 8 lines from normal lungs. We found that the fibroblasts from IPF spontaneously produced higher amounts of type I collagen and had lower expression levels of SOCS1 than fibroblasts from normal lung. Following IFN-γ stimulation, the amount of SOCS1 mRNA expressed by IPF fibroblasts was comparable to that of normal fibroblasts. Thus, the extent of SOCS1 increase after stimulation by IFN … More -γ was significantly higher in IPF fibroblasts. The extent to which IFN-γ inhibited collagen production was also larger in IPF fibroblasts than in normal fibroblasts.
(3) By using mouse fibroblasts, we demonstrated the causal relationship between decreased expression of SOCS-1 and increased production of collagen: the deficiency of SOCS1 in fibroblasts resulted in increased collagen production, whereas overexpression of SOCS1 suppressed collagen production. IFN-γ suppressed spontaneous collagen production even in SOCS1-deficient fibroblasts, indicating that IFN-γ inhibition is SOCS1-independent. In contrast, IFN-γ suppressed the increase of collagen production induced by IL-4 in wild type fibroblasts but not SOCS1-deficient fibroblasts, suggesting IFN-γ acted exclusively via SOCS1 in this case.
(4) These results suggest that the exaggerated production of collagen observed in fibroblasts from IPF is causally related to the diminished expression of SOCS1, and IPF fibroblasts are more susceptible to IFN-γ because of decreased expression of SOCS1. Less

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (30 results)

All 2007 2006 2005 2004 Other

All Journal Article (30 results)

  • [Journal Article] Overproduction of collagen and diminished SOCS1 expression are causally linked in fibroblasts from idiopathic pulmonary fibrosis2007

    • Author(s)
      Shoda H, Yokoyama A, et al.
    • Journal Title

      Biochem Biophys Res Commun 353

      Pages: 1004-1010

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Overproduction of collagen and diminished SOCS1 expression are causally linked in fibroblasts from idiopathic pulmonary fibrosis2007

    • Author(s)
      Shoda H, Yokoyama A, Nishino R, Nakashima T, Ishikawa N, Haruta Y, Hattori N, Naka T, Kohno N.
    • Journal Title

      Biochem Biophys Res Commun 353

      Pages: 1004-1010

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Air Trapping in Patients with Asthma May Represent Remodeling of Small Airways.2006

    • Author(s)
      Ito R, Yokoyama A, et al.
    • Journal Title

      Journal of asthma 43

      Pages: 25-29

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Prognostic Value of Circulating KL-6 in Idiopathic Pulmonary Fibrosis2006

    • Author(s)
      Yokoyama A, et al.
    • Journal Title

      Respirology 11

      Pages: 164-168

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Anti-tumor effect of the anti-KL-6/MUC1 monoclonal antibody through exposure of surface molecules by MUC1 capping2006

    • Author(s)
      Doi M, Yokoyama A, et al.
    • Journal Title

      Cancer Science

      Pages: 420-429

    • NAID

      10018177101

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Air Trapping in Patients with Asthma May Represent Remodeling of Small Airways.2006

    • Author(s)
      Ito R, Yokoyama A, Hamada H, Yasuhara Y, Kohno N, Higaki J.
    • Journal Title

      J Asthma 43

      Pages: 25-29

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Prognostic Value of Circulating KL-6 in Idiopathic Pulmonary Fibrosis.2006

    • Author(s)
      Yokoyama A, Kondo K, Nakajima M, Matsushima T, Takahashi T, Nishimura M, Bando M, Sugiyama Y, Totani Y, Ishizaki T, Ichiyasu H, Suga M, Hamada H, Kohno N.
    • Journal Title

      Respirology 11

      Pages: 164-168

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Anti-tumor effect of the anti-KL-6/MUCI monoclonal antibody through exposure of surface molecules by MUC1 capping.2006

    • Author(s)
      Doi M, Yokoyama A, Kondo K, Ohnishi H, Ishikawa N, Hattori N, Kohno N.
    • Journal Title

      Cancer Sci 97

      Pages: 420-429

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Air Trapping in Patients with Asthma May Represent Remodeling of Small Airways2006

    • Author(s)
      Ito R
    • Journal Title

      J Asthma 43・2

      Pages: 125-129

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Adoptive Transfer of Th1 Clones Inhibits Eosinophilic Inflammation and Airway Hyperresponsiveness in a Murine Model of Asthma2005

    • Author(s)
      Irifune K, Yokoyama A, et al.
    • Journal Title

      European Respiratory Journal 25

      Pages: 653-659

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Polymorphism of MUC1 mucin gene is associated with susceptibility and prognosis of lung adenocarcinoma2005

    • Author(s)
      Mitsuta, K, Yokoyama A, et al.
    • Journal Title

      Oncology Reports 14

      Pages: 185-189

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Circulating aminopeptidase N/CD13 is an independent prognostic factor in patients with non-small cell lung cancer.2005

    • Author(s)
      Murakami H, Yokoyama A, et al.
    • Journal Title

      Clinical Cancer Research 11

      Pages: 8674-8679

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] KL-6, a Human MUC1 Mucin, Promotes Proliferation and Survival of Lung Fibroblasts2005

    • Author(s)
      Ohshimo S, Yokoyama A, et al.
    • Journal Title

      Biochem Biophys Res Commun 338

      Pages: 1845-1852

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Adoptive Transfer of Th1 Clones Inhibits Eosinophilic Inflammation and Airway Hyperresponsiveness in a Murine Model of Asthma2005

    • Author(s)
      H, Hamada H, Nakajima M, Kohno N, Higaki J.
    • Journal Title

      Eur Respir J 25

      Pages: 653-659

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Polymorphism of MUC1 mucin gene is associated with susceptibility and prognosis of lung adenocarcinoma.2005

    • Author(s)
      Mitsuta K, Yokoyama A, Kondo K, Nakajima M, Kohno N.
    • Journal Title

      Oncol Rep 14

      Pages: 185-189

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Circulating aminopeptidase N/CD13 is an independent prognostic factor in patients with non-small cell lung cancer.2005

    • Author(s)
      Murakami H, Yokoyama A, Kondo K, Nakanishi S, Kohno N, Miyake M.
    • Journal Title

      Clin Cancer Res 11

      Pages: 8674-8679

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] KL-6, a Human MUCI Mucin, Promotes Proliferation and Survival of Lung Fibroblasts.2005

    • Author(s)
      Ohshimo S, Yokoyama A, Hattori N, Ishikawa N, Hirasawa Y, Kohno N.
    • Journal Title

      Biochem Biophys Res Comm 338

      Pages: 1845-1852

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Adoptive Transfer of Th1 Clones Inhibits Eosinophilic Inflammation and Airway Hyperresponsiveness in a Murine Model of Asthma2005

    • Author(s)
      Irifune K
    • Journal Title

      Eur Respir J 25・4

      Pages: 653-659

    • Related Report
      2005 Annual Research Report
  • [Journal Article] KL-6, a Human MUC1 Mucin, Promotes Proliferation and Survival of Lung Fibroblasts.2005

    • Author(s)
      Ohshimo S
    • Journal Title

      Biochem Biophys Res Comm 338・4

      Pages: 1845-1852

    • Related Report
      2005 Annual Research Report
  • [Journal Article] IL-10/IL-12軸を介した新たな気道過敏性の制御機構2005

    • Author(s)
      横山彰仁
    • Journal Title

      呼吸 24・3

      Pages: 201-208

    • Related Report
      2005 Annual Research Report
  • [Journal Article] NKT細胞と気管支喘息:病態発現におけるNKT細胞の関与2005

    • Author(s)
      横山彰仁
    • Journal Title

      分子呼吸器病 9・4

      Pages: 320-324

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Circulating Natural Killer T Cells in Patients with Asthma2004

    • Author(s)
      Ikegami Y, Yokoyama A, et al.
    • Journal Title

      Journal of Asthma 41

      Pages: 877-882

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] SOCS-1ノックアウトマウス2004

    • Author(s)
      庄田浩康, 横山彰仁
    • Journal Title

      分子呼吸器病 8

      Pages: 503-508

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Circulating Natural Killer T Cells in Patients with Asthma Irifune K, Yokoyama A, Sakai K, Watanabe A, Katayama H, Ohnishi2004

    • Author(s)
      Ikegami Y, Yokoyama A, Haruta Y, Hiyama K, Mendoza C, Kohno K.
    • Journal Title

      J Asthma 41

      Pages: 877-882

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 気管支喘息とロイコトリエン拮抗薬の臨床2004

    • Author(s)
      横山彰仁
    • Journal Title

      日本内科学会雑誌 93

      Pages: 2656-2661

    • NAID

      10014403208

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Overexpression of Suppressor of Cytokine Signaling-5 Augments Eosinophilic Airway Inflammation in Mice

    • Author(s)
      Ohshima M, Yokoyama A, et al.
    • Journal Title

      Clinical Experimental Allergy In press

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Circulating KL-6/MUC1 mucin carrying sialyl Lewisa oligosaccharide is an independent prognostic factor in patients with lung adenocarcinoma

    • Author(s)
      Inata J, Hattori N, Yokoyama A, et al.
    • Journal Title

      International Journal of Cancer In press

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Overexpression of Suppressor of Cytokine Signaling-5 Augments Eosinophilic Airway Inflammation in Mice

    • Author(s)
      Ohshima M, Yokoyama A, Ohnishi H, Hamada H, Kohno N, Higaki J, Naka T.
    • Journal Title

      Clin Exp Allergy (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Overexpression of Suppressor of Cytokine Signaling-5 Augments Eosinophilic Airway Inflammation in Mice

    • Author(s)
      Ohshima M, Yokoyama A, et al.
    • Journal Title

      Clinical Experimental Allergy (In press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Circulating KL-6/MUC1 mucin carrying sialyl Lewisa oligosaccharide is an independent prognostic factor in patients with lung adenocarcinoma

    • Author(s)
      Inata J, Hattori N, Yokoyama A, et al.
    • Journal Title

      International Journal of Cancer (In press)

    • Related Report
      2006 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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