| Project/Area Number |
16590756
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Oita University |
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Principal Investigator |
KADOTA Jun-ichi Oita University, Faculty of Medicine, Professor, 医学部, 教授 (50233838)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Pseudomonas aeruginosa / Twitching motility / macrolide antibiotics / chronic respiratory infection / carbocystein / biofilm / Th1 immune response / Th2 immune response / twitching motility / interferon-γ / interletukin-4 / RANTES / Th1細胞 / Th2細胞 |
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| Research Abstract |
1)To investigate the pathogenesis of twitching motility, a murine model of chronic respiratory infection was established by intratracheal inoculation of wild or mutant strain of twitching motility of P.aeruginosa. The results indicate that twitching motility deeply related to the development of chronic respiratory infection through shifting the host defence from Th1 to Th2.
2)In this model infected with wild strain, 12-week administration of macrolide antibiotic increased mice survival by inducing IFN-γ producing Th1 immune response in parallel with the manner seen in th model with mutant strain. Furthermore, in vitro study demonstrated the inhibition of P.aeruginosa twitching motility by 14- and 15-membered ring macrolides. This suggest that the suppression of twitching motility is one of the mechanism by which macrolide antibiotics have a therapeutic effect on chronic P.aeruginosa respiratory infection.
3)In addition, 12-week carbocystein treatment also inhibited biofilm formation of P.aeruginosa on plastic tube inserted into trachea of mice. The drug significantly inhibited in vitro biofilm formation of the bacteria. This result may provide another therapeutic strategy for chronic P.aeruginosa respiratory infection.
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