| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Research Abstract |
Aldose reductase (AR) and aldehyde reductase (ALR) are members of the aldo-keto reductase superfamily, and participate in the reduction of a wide range of carbonyl compounds. However, the roles of these reductases in the renal disease are unclear. In this study, we examined the expressions of AR and ALR in the rat remnant kidney at 1, 2, 4 and 10 weeks after subtotal nephrectomy. Expressions of AR and ALR are evaluated by Western blot analysis and immunostaining of renal tissue. In remnant kidney increased AR expression was detected at 2 weeks and AR expression was time-dependently increased during the progression of renal failure. In contrast, in sham-operated kidney AR expression was unchanged. Interestingly, ALR expressions were changed neither in remnant kidney nor in sham-operated kidney up to 10 weeks after surgery. Immunohistochemical examination revealed that in sham-operated rats AR expression was weakly detected in distal tubular epithelial cells, Henle's loop and podocytes. However, in remnant kidney increased AR expression was detected in glomerular endothelial cells in addition to them. ALR expression was observed in proximal tubules in both sham-operated and nephrectomized rats. The serum levels of reactive carbonyl compounds were higher in nephrectomized rats than in sham-operated rats. Our results indicate that AR and ALR show different responses to renal injury induced by renal mass reduction. This suggested that AR may play an important role to protect kidneys from carbonyl stress caused by renal failure. Furthermore, the expression of other anti-oxidative enzymes including thioredoxin reductase and peroxiredoxins were also detected in remnant kidney.
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