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ROLE OF KLOTHO GENE IN RENAL PROTECTION

Research Project

Project/Area Number 16590776
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionGunma University

Principal Investigator

SAITO Yuichiro  GUNMA UNIVERSITY, SCHOOL OF MEDICINE, ASSISTANT, 医学部, 助手 (30344922)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsENDOTHELIUM / NITRIC OXIDE / OXIDATIVE STRESS / ALUBUMIN URIA / REMODELING / HYPERTENSION / 加齢 / 高血圧 / アルブミン排泄 / 腎不全
Research Abstract

The expression of klotho mRNA in the kidney was significantly reduced in Dahl salt-sensitive hypertensive (Dahl-S) rats fed high salt (8% NaCl) diet as compared to those fed low salt (0.3% NaCl) diet (n=7). The klotho gene delivery once a week for 3 weeks induced klotho mRNA expression at the injection site. Hydroethidine, an oxidative fluorescent dye, was used to evaluate the levels of superoxide in situ. Intramuscular induction of the klotho gene decreased in superoxide production in the aorta, resulting in restoration of endothelial dysfunction from 43±6 % to 61±9 % of control rats (n=6, p<0.05). At the same time, systolic blood pressure was reduced from 262±11 mmHg to 234±6 mmHg (p<0.05). Furthermore, klotho gene delivery improved perivascular fibrosis from 0.031±0.002 to 0.011±0.001 mm^2 and reduced albuminuria from 2.5±0.4 to 0.31±0.14 g/day (n=8, p<0.05).
Our study indicates that intramusclar delivery of the klotho gene reduces oxidative stress and protects the vascular endothelium in Dhal-S rats. We suggest that induction of the klotho gene expression associated with the improvement of the endothelial function may be a potential novel therapeutic strategy for renal failure induced by hypertension.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (19 results)

All 2006 2005 2004 Other

All Journal Article (18 results) Book (1 results)

  • [Journal Article] 老化抑制遺伝子 klotho と血管内皮機能2006

    • Author(s)
      斎藤勇一郎, 倉林正彦, 中村哲也, 永井良三
    • Journal Title

      日本老年医学会誌 43(印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Klotho遺伝子2006

    • Author(s)
      斎藤勇一郎, 倉林正彦, 中村哲也, 永井良三
    • Journal Title

      日本臨床 64(印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Fluvastatin ameliorates hyperhomocysteinemia-induced endothelial dysfunction : The antioxidant properties of fluvastatin2005

    • Author(s)
      Morita H, Saito Y, Ohashi N, Katoh M, Ashida T, Kurihara H, et al.
    • Journal Title

      Circulation Journal 69

      Pages: 475-475

    • NAID

      110002696125

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Selective angiotensin receptor antagonism with valsartan decreases arterial stiffness independently of blood pressure lowering in hypertensive patients2005

    • Author(s)
      Nakamura T, Fujii S, Hoshino J, Saito Y, Mizuno H, Saito Y, Kurabayashi M.
    • Journal Title

      Hypertension Research 28

      Pages: 937-937

    • NAID

      10017403402

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Selective angiotensin receptor antagonism with valsartan decreases arterial stiffness independently of blood pressure lowering in hypertensive patients.2005

    • Author(s)
      Nakamura T, Fujii S, Hoshino J, Saito Y, Mizuno H, Saito Y, Kurabayashi M
    • Journal Title

      Hypertension Research 28

      Pages: 937-943

    • NAID

      10017403402

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Selective angiotensin receptor antagonism with valsartan decreases arterial stiffness independently of blood pressure lowering in hypertensive patients2005

    • Author(s)
      Nakamura T, Fujii S, Hoshino J, Saito Y, Mizuno H, Saito Y, Kurabayashi M.
    • Journal Title

      Hypertens Research 28

      Pages: 937-937

    • NAID

      10017403402

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Klothoマウス2005

    • Author(s)
      斎藤勇一郎, 倉林正彦
    • Journal Title

      循環器研究の動物たち 1

      Pages: 1-1

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 老化抑制遺伝子klothoと血管内皮機能2005

    • Author(s)
      斎藤勇一郎, 倉林正彦, 中村哲也, 永井良三
    • Journal Title

      日本老年医学会誌 43(印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effect of cerivastatin on endothelial dysfunction and aortic CD36 expression in diabetic hyperlipidemic rats2004

    • Author(s)
      Nakamura T, SaitoY, OhyamaY, Uchiyama T, Sumino H, Kurabayashi M
    • Journal Title

      Hypertension Resarch 27

      Pages: 589-589

    • NAID

      10013604297

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Klotho遺伝子2004

    • Author(s)
      斎藤勇一郎, 倉林正彦, 永井良三
    • Journal Title

      日本臨床 62

      Pages: 65-65

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] 尿中微量アルブミンと高血圧2004

    • Author(s)
      斎藤勇一郎, 中村哲也, 倉林正彦
    • Journal Title

      臨床検査 48

      Pages: 563-563

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] ホモシステイン2004

    • Author(s)
      斎藤勇一郎, 中村哲也, 倉林正彦, 森田啓行, 永井良三
    • Journal Title

      日本臨床 臨時増刊号3 62

      Pages: 211-211

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] klotho遺伝子2004

    • Author(s)
      斎藤勇一郎, 中村哲也, 倉林正彦, 永井良三
    • Journal Title

      日本臨床 臨時増刊号3 62

      Pages: 112-112

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] 虚血性心疾患を伴う高血圧症2004

    • Author(s)
      斎藤勇一郎, 中村哲也, 倉林正彦
    • Journal Title

      実験治療 673

      Pages: 6-6

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effect of cerivastatin on endothelial dysfunction and aortic CD36 expression in diabetic hyperlipidemic rats.2004

    • Author(s)
      Nakamura T, Saito Y, Ohyama Y, Uchiyama T, Sumino H, Kurabayashi M
    • Journal Title

      Hypertension Resarch 27

      Pages: 589-597

    • NAID

      10013604297

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effect of cerivastatin on endothelial dysfunction and aortic CD36 expression in diabetic hyperlinidemic rats2004

    • Author(s)
      Nakamura T, Saito Y, Ohyama Y, Uchiyama T, Sumino H, Kurabayashi M
    • Journal Title

      Hypertension Research 27

      Pages: 589-589

    • Related Report
      2004 Annual Research Report
  • [Journal Article] ホモシステイン2004

    • Author(s)
      斎藤勇一郎, 中村哲也, 倉林正彦, 森田啓行, 永井良三
    • Journal Title

      日本臨床 62

      Pages: 211-211

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Fluvastatin ameliorates hyperhonocysteinemia-induced endothelial dysfunction : The antioxidant nronerties of fluyastatin

    • Author(s)
      Morita H, Saito Y, Ohashi N, Katoh M, Ashida T, Kurihara H, et al.
    • Journal Title

      Circulation Journal (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Book] 循環器研究の動物たち2005

    • Author(s)
      斎藤勇一郎, 倉林正彦
    • Total Pages
      4
    • Publisher
      老化抑制遺伝子klothoと血管内皮機能
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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