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Mutltiplexin collagen family in renal tissues of diabetes patients and the application for diagnosis of diabetes nephropathy

Research Project

Project/Area Number 16590788
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionOkayama University

Principal Investigator

NAITO Ichiro  Okayama University, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Research Associate, 大学院医歯薬学総合研究科, 助手 (60362993)

Co-Investigator(Kenkyū-buntansha) NINOMIYA Yoshifumi  Okayama University, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Professor, 大学院医歯薬学総合研究科, 教授 (70126241)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsDiabetes mellitus / collagen / MULTIPLEXIN / Type XV / Type XVIII / monoclonal antibody / Diabetes nephropathy / diagnosis / XV型 / XVIII型
Research Abstract

We have shown a possibility of MULTIPLEN collagens as diagnostic markers of nephropathy in Diabetic patients. MULTIPLEXIN is a group of collagens, and type XV and type XVIII collagens are listed. They are characterized with large non collagenous domains in both N-and C-termini, in which endostatin and endostatin-like peptides are found in the C-termini, respectively. We have established monoclonal antibodies specific for both collagens. They were applied to immunohistochemistry and ELISA measurement.
In normal glomeruli, collagen type XVIII was found strongly in Bowman' s capsule and moderately in glomerular basement membrane, and collagen type XV was negative at all. In diabetic glomeruli, both collagens were increased in intensity of immunostaining. Especially, type XV collagen was increased exclusively in Mesangial matrix.
ELISA measurement was succeeded in collagen type XVIII but not in collagen type XV. We have measured urinary endostatin of collagen type XVIII in the urine of Diabetic patients. The amount of urinary collagen type XVIII was increased corresponding to the severity of nephropathy.
MULTIPLEXIN collagens are available as the markers for diabetic nephropathy ; collagen type XV is a marker for immunohistochemistry of renal biopsy, and collagen type XVIII is for the ELISA measurement of the urine.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (25 results)

All 2006 2005 2004

All Journal Article (24 results) Book (1 results)

  • [Journal Article] In situ preparation of colloidal iron by microwave irradiation for transmission electron microscopy.2006

    • Author(s)
      Nakatani S, Naito I, Momota R, Hinenoya N, Horiuchi K, Nishida K, Ohtsuka A.
    • Journal Title

      Acta Med. Okayama 60(1)

      Pages: 59-64

    • NAID

      120002305528

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Differential expression of basement membrane collagen-IV alphal to alpha6 chains during oral carcinogenesis2006

    • Author(s)
      Tamamura R, Nagatsuka H, Siar CH, Katase N, Naito I, Sado Y, Nagai N.
    • Journal Title

      Virchows Arch. 449(3)

      Pages: 358-366

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Differential expression of basement membrane collagen-IV alpha1 to alpha6 chains during oral carcinogenesis2006

    • Author(s)
      Tamamura R, Nagatsuka H, Siar CH, Katase N, Naito I, Sado Y, Nagai N.
    • Journal Title

      Virchows Arch 449(3)

      Pages: 358-66

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Loss of expression of type IV collagen alpha5 and alpha6 chains in colorectal cancer associated with the hypermethylation of their premoter region.2006

    • Author(s)
      Ikeda K, Iyama K, Ishikawa N, Egami H, Nakao M, Ninomiya Y, et al.
    • Journal Title

      Am J Pathol. 168(3)

      Pages: 856-865

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Immunolocalization and distribution patterns of type IV collagen alpha chains in oral mucosal melanoma.2005

    • Author(s)
      Nagatsuka H, Santos Rivera R, Gunduz M, Naito I, et al.
    • Journal Title

      Virchows Arch 447(4)

      Pages: 710-716

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Quantitative immunoelectron-microscopic analysis of the type IV collagen alphal-6 chains in the glomerular basement membrane in childhood thin basement membrane disease.2005

    • Author(s)
      Akazawa H, Nakajima M, Nishiguchi M, Naito I, et al.
    • Journal Title

      Clin Nephrol. 64(5)

      Pages: 329-336

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Three-dimensional ultrastructure of the brush border glycocalyx in the mouse small intestine : a high resolution scanning electron microscopic study.2005

    • Author(s)
      Horiuchi K, Naito I, Nakano K, Nakatani S, Taguchi T, et al.
    • Journal Title

      Arch Histol Cytol. 68(1)

      Pages: 51-56

    • NAID

      10015564977

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Alport症候群に合併した食道びまん性平滑筋腫症の1例.2005

    • Author(s)
      西江 学, 猶本良夫, 白川靖博, 内藤一郎, 二宮善文, 他
    • Journal Title

      消火器外科 28(12)

      Pages: 1829-1833

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Alport症候群における遺伝子型と表現型2005

    • Author(s)
      片山 鑑, 堅村信介, 内藤一郎, 井上実千代, 中野 赳
    • Journal Title

      腎と透析 58(3)

      Pages: 311-315

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] IV型コラーゲンα3,4,5鎖2005

    • Author(s)
      内藤一郎, 堅村信介
    • Journal Title

      腎と透析 59(2)

      Pages: 170-179

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Immunolocalization and distribution patterns of type IV collagen alpha chains in oral mucosal melanoma.2005

    • Author(s)
      Nagatsuka H, Santos Rivera R, Gunduz M, Lee YJ, Tamamura R, Gunduz E, Naito I, Sado Y, Nagai N.
    • Journal Title

      Virchows Arch. 447(4)

      Pages: 710-6

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Quantitative immunoelectron-microscopic analysis of the type IV collagen alphal-6 chains in the glomerular basement membrane in childhood thin basement membrane disease2005

    • Author(s)
      Akazawa H, Nakajima M, Nishiguchi M, Yamoto Y, Naito I, et al.
    • Journal Title

      Cl in Nephrol. 64(5)

      Pages: 329-36

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Three-dimensional ultrastructure of the brush border glycocalyx in the mouse small intestine : a high resolution scanning electron microscopic study.2005

    • Author(s)
      Horiuchi K, Naito I, Nakano K, Nakatani S, Nishida K, et al.
    • Journal Title

      Arch Histol Cytol. 68(1)

      Pages: 51-6

    • NAID

      10015564977

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A case of esophageal diffuse leiomyomatosis with Alport syndrome2005

    • Author(s)
      Nishie M, Naomoto Y, Shirakawa Y, Yamatsuji T, Asami S, Tanaka N, Naito I, Oohashi T, Ninomiya Y
    • Journal Title

      Shoukaki-Geka 28(12)

      Pages: 1829-1833

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Genotype and phenotype correlation in Alport syndrome2005

    • Author(s)
      Katayama K, Nomura S, Naito I, et al.
    • Journal Title

      Kidney and Dialysis 58(3)

      Pages: 311-315

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Type IV collagen alpha 3/ alpha 4/ alpha 5 molecule2005

    • Author(s)
      Naito I, Nomura S
    • Journal Title

      Kidney and Dialysis 59(2)

      Pages: 170-179

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] In situ preparation of colloidal iron by microwave irradiation for transmission electron microscopy.2005

    • Author(s)
      Nakatani S, Naito I, Momota R, Hinenoya N, Horiuchi K, Nishida K, Ohtsuka A.
    • Journal Title

      Acta Med Okayama 60(1)

      Pages: 59-64

    • NAID

      120002305528

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 正常〜微量アルブミン尿期糖尿病腎症におけるXV・XVIII型コラーゲンの局在2005

    • Author(s)
      田中啓司, 守屋達美, 内藤一郎, 他
    • Journal Title

      日本腎臓学会誌 47(3)

      Pages: 316-316

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Quantitative immunoelectron-microscopic analysis of the type IV collagen alpha1-6 chains in the glomerular basement membrane in childhood thin basement membrane disease2005

    • Author(s)
      Akazawa H, Nakajima M, Nishiguchi M, Yamoto Y, Naito I, et al.
    • Journal Title

      Clinical Nephrology. 64(5)

      Pages: 329-336

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Immunolocalization and distribution patterns of type IV collagen alpha chains in oral mucosal melanoma.2005

    • Author(s)
      Nagatsuka H, Santos Rivera R, Gunduz M, Naito I, Nagai N, et al.
    • Journal Title

      Virchows Arch. 447(4)

      Pages: 710-716

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Three-dimensional ultrastructure of the brush border glycocalyx in the mouse small intestine : a high resolution scanning electron microscopic study.2005

    • Author(s)
      Horiuchi K, Naito I, Nakano K, Nakatani S, Ohtsuka A, et al.
    • Journal Title

      Arch Histol Cytol. 68(1)

      Pages: 51-56

    • NAID

      10015564977

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Decreased expression of B7 costimulatory molecules and major histocompatibility complex class-I in human hepatocellular carcinoma2004

    • Author(s)
      Fujiwara K, Higashi T, Naito I, et al.
    • Journal Title

      J Gastroenterol Hepatol. 19

      Pages: 1121-1127

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Immunohistochemical localization of type IV collagen alpha chains in the basement membrane of the pancreatic duct in human normal panc2004

    • Author(s)
      Kadono G, Ishihara T, Naito I, et al.
    • Journal Title

      Pancreas 29

      Pages: 61-66

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 遺伝性腎症に伴う病変-Alport症候群2004

    • Author(s)
      内藤一郎, の村信介
    • Journal Title

      腎と透析 56

      Pages: 170-179

    • Related Report
      2004 Annual Research Report
  • [Book] "Disorder of tubular transport, Fanconi Syndrome, Nephrotic Cystinosis, and Mitochondrial disease" in "Renal Diseases"2005

    • Author(s)
      内藤一郎
    • Total Pages
      7
    • Publisher
      東京医学社(予定)
    • Related Report
      2004 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

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