| Project/Area Number |
16590807
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | University of Occupational and Environmental Health |
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Principal Investigator |
SERINO Ryota (2005) University of Occupational and Environmental Health, School of Medicine, Research Associate, 医学部, 助手 (00309957)
穴井 博史 (2004) 産業医科大学, 医学部, 助手 (40279328)
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| Co-Investigator(Kenkyū-buntansha) |
TSUTSUI Masato University of Occupational and Environmental Health, School of Medicine, Associate Professor, 医学部, 助教授 (70309962)
TAMURA Masahito University of Occupational and Environmental Health, University Hospital Kidney Center, Associate Professor, 大学病院, 助教授 (40330980)
芹野 良太 産業医科大学, 医学部, 助手 (00309957)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Nitric oxide synthase / Renal failur / Glomerulosclerosis / 神経型NO合成酵素 |
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| Research Abstract |
We reported the development of mice in which all three NOS isoforms are completely disrupted. Glomerulosclerosis and glomerular thrombus formation were noted in the triply n/i/eNOS^<-/-> mice but not in the wild-type mice. Quantitative analysis revealed that abnormalities, including renal tubular apotosis and glomerulosclerosis, were exacerbated in accordance with the number of disrupted NOS genes. These results suggest the NOS system plays an important role in the progression of glomerular diseases. We investigated body water balance. Urinary volume, water intake, and plasma osmolality were all significantly increased in accordance with the number of disrupted NOS genes. The triply n/i/eNOS^<-/-> mice reduced antidiuretic response to exogenous vasopressin, accompanied by impaired renal camp production. Therefore, the NOS system plays a role in maintaining homeostasis, especially in the kidney.
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