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The role of inflammation on development of Alzheimer's dementia

Research Project

Project/Area Number 16590815
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurology
Research InstitutionNiigata University

Principal Investigator

MIIDA Takashi  Niigata University, Medical and Dental Hospital, Lecturer, 医歯学総合病院, 講師 (80260545)

Co-Investigator(Kenkyū-buntansha) YAMADA Toshiyuki  Jichi Medical School, Department of Clinical Pathology, Associate Professor, 医学部, 助教授 (50211636)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsAlzheimer's dementia / Inflammation / Lipoprotein / Cerebrospinal fluid / HDL / Apolipoprotein E / アルツハイマー型痴呆
Research Abstract

Objectives : SAA, one of the acute phase proteins, is expressed in central nervous system, and increases in the brain of Alzheimer's dementia. This study was aimed to investigate whether SAA dissociates apolipoproteins from cerebrospinal HDL (CSF-HDL) in dose- and time-dependent manners, and if so, whether the dissociated apolipoproteins can be bound to amyloid-β.
Methods : CSF was pooled from neurologically normal subjects. We added PBS or SAA to the pooled CSF. In some experiments, we added amyloid-β to CSF that was mixed with PBS or SAA. The mixture was incubated at 37℃. The distribution of apolipoproteins or amyloid-β in CSF-HDL was analyzed by non-denaturing two-dimensional gel electrophoresis and Western blotting.
Results : SAA but not PBS dissociated apoE, apoAI, and apoAI from CSF-HDL immediately. The SAA concentration required for dissociation of apolipoproteins was the lowest in apoE, the highest in apoAII, and intermediate in apoAI. The more SAA added, the more apolipoproteins dissociated from CSF-HDL. After 2-h incubation, apoE and apoAI further dissociated from CSF-HDL. Amyloid-β was bound to CSF-HDL after 3h-incubation. However, amyloid-β was not bound to the dissociated apolipoproteins.
Conclusions : SAA dissociates apolipoproteins from CSF-HDL in dose- and time-dependent manners. The dissociated apolipoproteins are not bound to amyloid-β. We speculate that chronic mild inflammation in the central nervous system might dissociate apoE form CSF-HDL, and decrease the clearance of amyloid-β from CSF.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (31 results)

All 2006 2005 2004 Other

All Journal Article (30 results) Book (1 results)

  • [Journal Article] Estimating the fasting triglyceride concentration from the post-prandial HDL-cholesterol and apolipoprotein CIII concentrations.2006

    • Author(s)
      Kosuge K, et al.
    • Journal Title

      Atherosclerosis 184・2

      Pages: 413-419

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Estimating the fasting triglyceride concentration from the postprandial HDLcholesterol and apolipoprotein CIII concentrations.2006

    • Author(s)
      Kosuge K, et al.
    • Journal Title

      Atherosclerosis Vol.184

      Pages: 413-419

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Estimating the fasting triglyceride concentration from the postprandial HDLcholesterol and apolipoprotein CIII concentrations.2006

    • Author(s)
      Kosuge K, et al.
    • Journal Title

      Atherosclerosis 184・2

      Pages: 413-419

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Can stating really reduce the risk of Alzheimer's disease and slow its progression?2005

    • Author(s)
      T.Miida, et al.
    • Journal Title

      Current Opinion in Lipidology 16・6

      Pages: 619-623

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effect of apolipoprotein E (apoE) phonotypes on apoE content of CSF-HDL in children.2005

    • Author(s)
      Hirayama S, et al.
    • Journal Title

      Clinica Chimica Acta 356・1-2

      Pages: 110-116

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Circadian rhythm of protein C in human plasma : Useful marker of autonomic function in liver.2005

    • Author(s)
      Obayashi K, et al.
    • Journal Title

      Clinical Autonomic Research 15・1

      Pages: 57-58

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Bezafibrate suppresses rat anti-glomerular basement membrane crescentic glomerulonephritis.2005

    • Author(s)
      Saga D, et al.
    • Journal Title

      Kidney International 67・5

      Pages: 1821-1829

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Prediction of the three-dimensional structure of proteins in the lipid monolayer on the basis of a-helix binding. The apolipoprotein B-100 structure.2005

    • Author(s)
      Zhu Y, et al.
    • Journal Title

      Acta Medica et Biologica 53・2

      Pages: 51-60

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Can statins really reduce the risk of Alzheimer's disease and slow its progression?2005

    • Author(s)
      Miida T, et al.
    • Journal Title

      Current Opinion in Lipidology Vol.16

      Pages: 619-623

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Circadian rhythm of protein C in human plasma : Useful marker of autonomic function in liver.2005

    • Author(s)
      Obayashi K, et al.
    • Journal Title

      Clinical Autonomic Research Vol.15

      Pages: 57-58

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Bezafibrate suppresses rat anti-glomerular basement membrane crescentic glomerulonephritis.2005

    • Author(s)
      Saga D, et al.
    • Journal Title

      Kidney International Vol.67

      Pages: 1821-1829

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effect of apolipoprotein E (apoE) phonotypes on apoE content of CSF-HDL in children.2005

    • Author(s)
      Hirayama S, et al.
    • Journal Title

      Clinica Chimica Acta Vol.356

      Pages: 110-116

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Prediction of the three-dimensional structure of proteins in the lipid monolayer on the basis of a-helix bundling : Apolipoprotein B-100 structure.2005

    • Author(s)
      Zhu Y, et al.
    • Journal Title

      Acta Medica et Bologica Vol.53

      Pages: 51-60

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Can statins really reduce the risk of Alzheimer's disease and slow its progression?2005

    • Author(s)
      T.Miida, et al.
    • Journal Title

      Current Opinion in Lipidology 16・6

      Pages: 619-623

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Lipoprotein Lipase (LPL) mass in pre heparin serum reflects insulin sensitivity.2004

    • Author(s)
      O.Hanyu, et al.
    • Journal Title

      Atherosclerosis 174・2

      Pages: 385-390

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] T13M mutation of lecithin-cholesterol acyltransferase gene causes fisheye disease.2004

    • Author(s)
      T.Miida, et al.
    • Journal Title

      Clinica Chimica Acta 343・1-2

      Pages: 201-208

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] LCAT-dependent conversion rate is a determinant of plasma preβ1-HDL concentration in healthy Japanese.2004

    • Author(s)
      T.Miida, et al.
    • Journal Title

      Clinica Chimica Acta 350・1-2

      Pages: 107-114

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Cholesterol-independent effects of statins and its new therapeutic targets ischemic stroke and dementia.2004

    • Author(s)
      T.Miida, et al.
    • Journal Title

      Journal of Atherosclerosis and Thrombosis 11・5

      Pages: 253-264

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Lipid analyses for the management of vascular diseases.2004

    • Author(s)
      M.Okada, et al.
    • Journal Title

      Journal of Atherosclerosis and Thrombosis 11・4

      Pages: 190-199

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Lipoprotein Lipase (LPL) mass in pre-heparin serum reflects insulin sensitivity.2004

    • Author(s)
      Hanyu O, et al.
    • Journal Title

      Atherosclerosis Vol.174

      Pages: 385-390

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] T13M mutation of lecithin-cholesterol acyltransferase gene causes fish-eye disease.2004

    • Author(s)
      Miida T, et al.
    • Journal Title

      Clinica Chimica Acta Vol.343

      Pages: 201-208

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] LCAT-dependent conversion rate is a determinant of plasma preβ1-HDL concentration in healthy Japanese.2004

    • Author(s)
      Miida T, et al.
    • Journal Title

      Clinica Chimica Acta Vol.350

      Pages: 107-114

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cholesterol-independent effects of statins and its new therapeutic targets : ischemic stroke and dementia.2004

    • Author(s)
      Miida T, et al.
    • Journal Title

      Journal of Atherosclerosis and Thrombosis Vol.11

      Pages: 253-264

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Lipid analyses for the management of vascular diseases.2004

    • Author(s)
      Okada M, et al.
    • Journal Title

      Journal of Atherosclerosis and Thrombosis Vol.11

      Pages: 190-199

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Lipoprotein Lipase(LPL) mass in pre-heparin serum reflects insulin sensitiyity.2004

    • Author(s)
      O.Hanyu, et al.
    • Journal Title

      Atherosclerosis 174・2

      Pages: 385-390

    • Related Report
      2004 Annual Research Report
  • [Journal Article] T13M mutation of lecithin-cholesterol acyltransferase gene causes fish-eye disease.2004

    • Author(s)
      T.Miida, et al.
    • Journal Title

      Clinica Chimica Acta 343・1-2

      Pages: 201-208

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cholesterol-independent effects of statins and its new therapeutic targets : ischemic stroke and dementia.2004

    • Author(s)
      T.Miida, et al.
    • Journal Title

      Journal of Atherosclerosis and Thrombosis 11・5

      Pages: 253-264

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Preβ1-HDLの成熟障害を認めた魚眼病の一例.2004

    • Author(s)
      三井田孝他
    • Journal Title

      The Lipid 15・4

      Pages: 399-404

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Serum amyloid A (SAA)-induced remodeling of CSF-HDL.

    • Author(s)
      Miida T, et al.
    • Journal Title

      Biochimica et Biophysica Acta (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Serum amyloid A (SAA)-induced remodeling of CSF-HDL.

    • Author(s)
      Miida, et al.
    • Journal Title

      Biochimica et Biophysica Acta (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 人体の構造と機能及び疾病の成り立ち(脂質の輸送と蓄積を担当)2005

    • Author(s)
      三井田 孝, 他
    • Total Pages
      320
    • Publisher
      南江堂
    • Related Report
      2005 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

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