Mechanisms of production of two new microglial growth-differentiation factors from brain astrocytes and neurons

• Project/Area Number: 16590858
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurology
• Research Institution: Institute of Animal Reproduction (2005-2006); National Center of Neurology and Psychiatry (2004)
• Principal Investigator: KIKUCHI Aiko Institute of Animal Reproduction, Ph.D., Researcher, 実験研究センター, 主席研究員 (70159010)
• Project Period (FY): 2004 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥3,600,000 (Direct Cost: ¥3,600,000); Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: microglia / neuron / astrocyte / thymic myoid cell / monocyte / cytokine / autoimmune disease / myoastheniagrabis / 分化増殖因子 / クログリア / 脳 / 分化 / 増殖 / 胸腺 / 筋様細胞 / 細胞培養 / 総血因子 / thymic myoid cells / astrocyte / neuron / myathenia gravis / haemopoietic growth factor

Research Abstract

To elucidate the mechanism of the pathogenesis of myasthenia gravis, we studied physiological roles of thymic myoid cells. We found two new haemopoietic factors such as an 80-kDa and 100-kDa factor in myoid cells. The antibodies raised against these factors clearly localized clusters of precursor myoid cells in the hyperplastic thymi. Both factors were capable of stimulating the growth of B-cells in a synergistic fashion with other thymic cytokines. To understand the importance of numerical increase of myoid cell precursors, we studied transcription factors for muscle cells.
Interestingly,
the hyperplastic thymi expressed a particular type of transcription factor. These two factors were also detected in the brain cells such as glial cells and neuronal cells and play important roles in microglial cell growth and neural cell survival. In addition, we succeeded in producing recombinant products of the factors.

Research Products

• [Journal Article] Immunological and neurological roles of 80-kDa and 100-kDa haemopoietic factors. (2006)
  • Author(s): Kikuchi, A., Tomoyasu, H., Yamamoto, T., Kusakabe, M., Sakuragawa, N., Kamo, I.
  • Journal Title: J. Neuroimmunol. 178 Pages: 173-174
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Studies on myoid cells in hyperplastic myasthenia gravis (2006)
  • Author(s): Kikuchi, A., Tomoyasu, H., Yamamoto, T., Kusakabe, M., Sakuragawa, N., Kamo, I.
  • Journal Title: J. Neuroimmunol. 178 Pages: 228-228
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Immunological and neurological roles of 80-kDa and 100- kDa haemopoietic factors. (2006)
  • Author(s): Kikuchi, A., Tomoyasu, H., Kobayashi, M., Sakuragawa, N, Kamo, I.
  • Journal Title: J. Neuroimmunol. 178 Pages: 173-174
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Studies on myoid cells in hyperplastic myasthenia gravis (2006)
  • Author(s): Kamo, I., Tomoyasu, H., Kobayashi, M., Sakuragawa, N., Hagiwara, T., Kaneda, T., Kikuchi, A.
  • Journal Title: J. Neuroimmunol. 178 Pages: 228-228
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Immunological and neurological roles of 80-Kda and 100-kDa haemopoietic factors (2006)
  • Author(s): Kikuchi, A., Tomoyasu, H., Kobayashi, M., Sakuragawa, N., Kamo, I.
  • Journal Title: J. Immunol.
• [Journal Article] Studies on myoid cells in hyperplastic myasthenia gravis (2006)
  • Author(s): Kamo, I., Tomoyasu, H., Kobayashi, M., Sakuragawa, N., Hagiwara, T., Kaneda, T., Kikuchi, A.
  • Journal Title: J. Immunol.
• [Journal Article] Analysis of cytokine network for initiation of immune responses in the hyperplastic thymus associated with myasthenia gravis (2005)
  • Author(s): Kamo, I., Kikuchi, A., Tomoyasu, H., Sakuragawa, N.
  • Journal Title: J. Clinical Immunol. 193 Pages: 193-194
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Analysis of cytokine network for initiation of immune responses in the hyperplastic thymus associated with myasthenia gravis (2005)
  • Author(s): Kamo, I., Kikuchi, A., Tomoyasu, H., Sakuragawa, N.
  • Journal Title: J.Clinical Immunol. 193 Pages: 193-194
• [Journal Article] Human amnion mesenchyme cells express phenotypes of neurological progenitor cells (2004)
  • Author(s): Sakuragawa, N., Kakinuma, K., Kikuchi, A., Okano, H., Uchida, S., Kamo, I., Kobayashi, M., Yokoyama, Y.
  • Journal Title: J. Neuroscience Research 78 Pages: 208-214
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Biological roles of two new haemopoietic factors in brain and thymu (2004)
  • Author(s): Kikuchi, A., Tomoyasu, H., Yamamoto, T., Kusakabe, M., Sakuragawa, N., Kamo, I.
  • Journal Title: J. Neuroimmunol. 154 Pages: 34-34
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Mechanism of immune responses in myasthenic thymus (2004)
  • Author(s): Kamo, I., Tomoyasu, H., Yamamoto, T., Kusakabe, M., Kikuchi, A.
  • Journal Title: J. Neuroimmunol. 154 Pages: 96-96
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Biological roles of two new haemopoietic factors in brain and thymus (2004)
  • Author(s): Kikuchi, A., Tomoyasu, H., Yamamoto, T., Kusakabe, M., Sakuragawa, N., Kamo, I.
  • Journal Title: J. Neuroimmunol. 154 Pages: 34-34
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mechanism of immune responses in myasthenic thymus (2004)
  • Author(s): Kamo, I., Tomoyasu, H., Yamamoto, T., Kusakabe, M., Kikuchi, A
  • Journal Title: J. Neuroimmunol. 154 Pages: 96-96
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Biological roles of two new haemopoietic factors in brain and thymus (2004)
  • Author(s): Kikuchi, A., Tomoyasu, H., Yamamoto, T., Kusakabe, M.
  • Journal Title: J.Neruroimmunolgy 154 Pages: 34-34
• [Journal Article] Mechanism of immune responses in myasthenic thymus (2004)
  • Author(s): Kamo, I., Tomoyasu, H., Yamamoto, T., Kusakabe, M., Kikuchi, A.
  • Journal Title: J.Neruroimmunolgy 154 Pages: 96-96
• [Journal Article] Human amnion mesenchyme cells express phenotypes of neurological progenitor cells (2004)
  • Author(s): Sakuragawa, N., Kakinuma, K., Kikuchi, A., Okano, H., Uchida, S., Kamo, I., Kobayashi, M., Yokoyama, Y.
  • Journal Title: J.Neuroscience Research 78 Pages: 208-214
• [Journal Article] Immunological and neurological roles of 80-kDa and 100-kDa haemopoietic factors.
  • Author(s): Kikuchi, A., Tomoyasu, H., Kobayashi, M., Sakuragawa, N., Kamo,I.
  • Journal Title: Proceedings of 8th International Congress of Neuroimmunology, (Ed : T. Tabira) (Publisher : Medimond International Proceedings) (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Studies on myoid cells in hyperplastic myasthenia gravis
  • Author(s): Kamo, I., Tomoyasu, H., Kobayashi, M., Sakuragawa, N., Hagiwara, T., Kaneda, T., Kikuchi, A.
  • Journal Title: Proceedings of 8th International Congress of Neuroimmunology, (Ed : T. Tabira) (Publisher : Medimond International Proceedings) (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Immunological and neurological roles of 80-kDa and 100- kDa haemopoietic factors.
  • Author(s): Kikuchi, A., Tomoyasu, H., Kobayashi, M., Sakuragawa, N, Kamo, I.
  • Journal Title: In Press in Proceedings of 8th International Congress of Neuroimmunology, Ed : T. Tabira Publisher : Medimond International Proceedings
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Studies on myoid cells in hyperplastic myasthenia gravis
  • Author(s): Kamo, I., Tomoyasu, H., Kobayashi, M., Sakuragawa, N., Hagiwara, T., Kaneda, T., Kikuchi, A.
  • Journal Title: In Press in Proceedings of 8th International Congress of Neuroimmunology, Ed : T. Tabira Publisher : Medimond International Proceedings
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Immunological and neurological roles of 80-kDa and 100-kDa haemopoietic factors
  • Author(s): Kikuchi, A., Tomoyasu, H., Kobayashi, M., Sakuragawa, N., Kamo, I.
  • Journal Title: Proceedings of 8th International Congress of Neuroimmunology (Ed : T. Tabira)(Publisher : Medimond International Proceedings) (in press)
• [Journal Article] Studies on myoid cells in hyperplastic myasthenia gravis
  • Author(s): Kamo, I., Tomoyasu, H., Kobayashi, M., Sakuragawa, N., Hagiwara, T., Kaneda, T., Kikuchi, A.
  • Journal Title: Proceedings of 8th International Congress of Neuroimmunology (Ed : T. Tabira)(Publisher : Medimond International Proceedings) (in press)