Molecular Epidemiological Study for Genetic Factors Susceptible for Type 2 Diabetes
| Project/Area Number |
16590863
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Metabolomics
|
|---|
| Research Institution | Yamagata University |
|---|
Principal Investigator |
DAIMON Makoto Yamagata University, School of Medicine, Associate Professor, 医学部, 助教授 (20241698)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
加藤 丈夫 山形大学, 医学部, 教授 (90194828)
|
|---|
| Project Period (FY) |
2004 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
|
|---|
| Keywords | Association Study / SNP / Diabetes Mellitus / Haplotype Analysis / Diplotype Analysis / ABCA1 / Nephrin / ハプロタイプ |
|---|
| Research Abstract |
We have investigated genes and polymorphisms that associate with type 2 diabetes (DM) in the Japanese population (first and second sample sets (DM:72, IGT:75, and NGT:227 and DM:31, IGT:77 and NGT:244, respectively)), and found 33 SNPs (29 genes) out of 2039 SNPs (704 genes) to be significantly associated with DM. Among them, we further examined the association of the ATP-binding cassette transporter Al (ABCA1) and Nephrin genes with DM in details by studying genetic polymorphisms of the genes including its linkage disequilibrium (LD) and haplotype analyses. Results-1. ABCA1 : Genotypes of 34 SNPs distributed from the promoter region to the last exon of the ABCA1 gene revealed 13 LD blocks in the genes. An LD block at the 5'-region showed a significant difference in the haplotype distribution between the study groups (NGT vs. IGT+DM : overall p=0.0180 ; NGT vs. DM : 0.0001). The Fisher's exact probability test (NGT vs. DM) showed a significant association of the haplotype 2 of the LD block (p=0.0001), with an odds ratio (OR) of 2.53 (95%CI : 1.62-4.12). Diplotype analysis also showed a significant association of the diplotypes with the haplotype 2 (OR/p : 2.59/0.0013). 2. Nephrin : The associations of three SNPs (C294T, -61C/G and C2289T) with DM were examined. In the first sample set, these SNPs showed a significant association (p=0.0028, 0.0336 and 0.0007, respectively). Haplotype analysis revealed one block with the six haplotypes. Haplotype 1 (composed of the protective alleles of each SNP) and Diplotype 1/1 showed significant associations (0.42/0.0008, and 0.35/0.0005, respectively). The association was further confirmed by analysis using the second sample set as well, and was significant even after the adjustment for TG, BMI and systolic blood pressure (0.38/<0.0001). Conclusions- The ABCA1 and the Nephrin genes were associated with DM ; thus, these genes may play an important role in the pathophysiology of DM.
|
Report
(3 results)
Research Products
(10 results)
-
-
[Journal Article] Correlation between change in body weight rather than current body weight and change in serum adiponectin levels in a Japanese population - The Funagata study.2006
Author(s)
Arawaka, N., Daimon.M., Oizumi, T., Jimbu, Y., Kameda, W., Yamaguchi, H., Ohnuma, H., Tominaga, M., Kato, K.
-
Journal Title
Metabolism 55
Pages: 324-330
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
-
[Journal Article] Association of the ABCA1 gene polymorphisms with type 2 DM in a Japanese population.2005
Author(s)
Daimon, M., Kido, T., Baba, M., Oizumi, T., Jimbu, Y., Kameda, W., Yamaguchi, H., Ohnuma, H., Tominaga, M., Muramatsu, M., Kato, T.
-
Journal Title
Biochemical and Biophysical Research Communications 329
Pages: 205-210
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Journal Article] Association of Decrease in Serum DHEAS Levels With the Progression to Type 2 Diabetes in Men of a Japanese Population - The Funagata Study.2005
Author(s)
Kameda, W., Daimon, M., Oizumi, T., Jimbu, Y., Kimura, M., Hirata, A., Yamaguchi, H., Ohnuma, H., Igarashi, M., Tominaga, M., Kato, K.
-
Journal Title
Metabolism 54
Pages: 669-676
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
-
[Journal Article] Association of the Nephrin Gene Polymorphisms With Type 2 Diabetes in a Japanese Population-The Funagata Study.
Author(s)
Daimon.M., Guijin Ji, Oizumi, T., Kido, T., Baba, M., Jimbu, Y., Kameda, W., Susa, S., Yamaguchi, H., Ohnuma, H., Muramatsu, M., Kato, T.
-
Journal Title
Description
「研究成果報告書概要(欧文)」より
Related Report
-
