• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The effects of partial disruption of tricarboxylic acid cycle on energy metabolism. The analysis of expression forms of citrate synthetase gene-disrupted mice

Research Project

Project/Area Number 16590864
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Metabolomics
Research InstitutionUNIVERSITY OF TSUKUBA

Principal Investigator

SONE Hirohito  University of Tsukuba, Graduate School of Comprehensive Human Sciences, Assistant Professor, 大学院・人間総合科学研究科, 講師 (30312846)

Co-Investigator(Kenkyū-buntansha) SHIMANO Hitoshi  University of Tsukuba, Graduate School of Comprehensive Human Sciences, Associate Professor, 大学院・人間総合科学研究科, 助教授 (20251241)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordstricarboxylic acid (TCA) cycle / citrate synthase / knockout mice / lifestyle-related disorders / KOマウス / エネルギー代謝
Research Abstract

To find out solution over lifestyle-related metabolic disorders like obesity, diabetes and dyslipidemia, it is necessary to re-evaluate the significance of tricarboxylic acid (TCA) cycle, an important metabolic pathway with very high efficiency. To investigate the effects of inhibiting the cycle, the gene encoding citrate synthase (CS), one of rate-limiting enzymes of the cycle, was disrupted in mice. Homozygous (CS-/-) mice showed early embryonic lethality but heterozygous mice (CS+/-) showed partial expression of the CS gene in each of the mRNA, protein, and enzyme activity levels. Whereas there were no differences in weight, basal metabolic rate, blood pressure, fasting plasma glucose/insulin levels, serum lipid levels between wild-type and CS+/- mice, it is clarified that respiratory quotient (respiratory exchange ratio) was significantly lower and serum acetoacetate levels were significantly higher in CS+/- mice. High-fat high-sugar diet of one or two months for both wild-type and CS+/- mice resulted in marked blunted plasma response after intravenous glucose load without any differences in glucose responses in CS+/- mice compared with wild-type mice. It is thought that the mouse could be a useful model to investigate the mechanisms of insulin resistance underlying energy metabolism disorders.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (18 results)

All 2006 2005 2004 Other

All Journal Article (18 results)

  • [Journal Article] TFE3 transcriptionally activates hepatic IRS-2, participates in insulin signaling and ameliorates diabetes.2006

    • Author(s)
      Nakagawa Y
    • Journal Title

      Nat Med. 12

      Pages: 107-113

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] The new worldwide definition of metabolic syndrome is not a better diagnostic predictor of cardiovascular disease in Japanese diabetic patients than the existing definitions. Additional analysis from the Japan Diabetes Complications Study.2006

    • Author(s)
      Sone H
    • Journal Title

      Diabetes Care 29

      Pages: 145-147

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] TFE3 transcriptionally activates hepatic IRS-2, participates in insulin signaling and ameliorates diabetes.2006

    • Author(s)
      Nakagawa Y, Shimano H, Yoshikawa T, Ide T, Tamura M, Furusawa M, Yamamoto T, Inoue N, Matsuzaka T, Takahashi A, Hasty AH, Suzuki H, Sone H, Toyoshima H, Yahagi N, Yamada N.
    • Journal Title

      Nat Med. 12

      Pages: 107-113

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The new worldwide definition of metabolic syndrome is not a better diagnostic predictor of cardiovascular disease in Japanese diabetic patients than the existing definitions. Additional analysis from the Japan Diabetes Complications Study.2006

    • Author(s)
      Sone H, Tanaka S, Ishibashi S, Yamasaki Y, Oikawa S, Ito H, Saito Y, Ohashi Y, Akanuma Y, Yamada N, Japan Diabetes Complications Study (JDCS) Group.
    • Journal Title

      Diabetes Care 29

      Pages: 145-147

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Transgenic mice overexpressing SREBP-1a under the control of the PEPCK promoter exhibit insulin resistance, but not diabetes.2005

    • Author(s)
      Takahashi A
    • Journal Title

      Biochim Biophys Acta 1740

      Pages: 427-433

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Is the diagnosis of metabolic syndrome useful for predicting cardiovascular disease in Asian diabetic patients? -Analysis from the Japan Diabetes Complications Study-2005

    • Author(s)
      Sone H
    • Journal Title

      Diabetes Care 28

      Pages: 1463-1471

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Transgenic mice overexpressing nuclear SREBP-1c in pancreatic beta-cells2005

    • Author(s)
      Takahashi A
    • Journal Title

      Diabetes 54

      Pages: 492-499

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Transgenic mice overexpressing SREBP-1a under the control of the PEPCK promoter exhibit insulin resistance, but not diabetes2005

    • Author(s)
      Takahashi A, Shimano H, Nakagawa Y, Yamamoto T, Motomura K, Matsuzaka T, Sone H, Suzuki H, Toyoshima H, Yamada N.
    • Journal Title

      Biochim Biophys Acta 1740

      Pages: 427-433

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Is the diagnosis of metabolic syndrome useful for predicting cardiovascular disease in Asian diabetic patients? -Analysis from the Japan Diabetes Complications Study-.2005

    • Author(s)
      Sone H, Mizuno S, Yoshimura Y, Yamazaki Y, Katayama S, Saito Y, Ohashi Y, Akanuma Y, Yamada N, Japan Diabetes Complications Study (JDCS) Group.
    • Journal Title

      Diabetes Care 28

      Pages: 1463-1471

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Transgenic mice overexpressing nuclear SREBP-1c in pancreatic beta-cells.2005

    • Author(s)
      Takahashi A, Motomura K, Kato T, Yoshikawa T, Nakagawa Y, Yahagi N, Sone H, Suzuki H, Toyoshima H, Yamada N, Shimano H.
    • Journal Title

      Diabetes 54

      Pages: 492-499

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Transgenic mice overexpressing nuclear SREBT-1c in pancreatic beta-cells.2005

    • Author(s)
      Takahashi A, et al.
    • Journal Title

      Diabetes 54

      Pages: 492-499

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Statins downregulate ATP binding cassette transporter A1 gene expression in macrophages.2004

    • Author(s)
      Sone H
    • Journal Title

      Biochem Biophys Res Commun 316

      Pages: 790-794

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Statins downregulate ATP binding cassette transporter Al gene expression in macrophages.2004

    • Author(s)
      Sone H, Shimano H, Shu M, Nakakuki M, Takahashi A, Sakai M, Sakamoto Y, Yokoo T, Matsuzaka K, Okazaki H, Nakagawa Y, Iida KT, Suzuki H, Toyoshima H, Horiuchi S, Yamada N.
    • Journal Title

      Biochem Biophys Res Commun 316

      Pages: 790-794

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] WGEF is a novel RhoGEF expressed in intestine, liver, heart, and kidney.2004

    • Author(s)
      Wang Y, et al.
    • Journal Title

      Biochem Biophys Res Commun 324

      Pages: 1053-1058

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Alcohol Use and Diabetes Mellitus.2004

    • Author(s)
      Sone H, et al.
    • Journal Title

      Ann Intern Med 141

      Pages: 408-409

    • Related Report
      2004 Annual Research Report
  • [Journal Article] SREBPs suppress IRS-2-mediated insulin signalling in the liver.2004

    • Author(s)
      Ide T, et al.
    • Journal Title

      Nat Cell Biol 6

      Pages: 351-357

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Statins downregulate ATP binding cassette transporter A1 gene expression in macrophages.2004

    • Author(s)
      Sone H, et al.
    • Journal Title

      Biochem Biophys Res Commun 316

      Pages: 790-794

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Is the diagnosis of metabolic syndrome useful for predicting cardiovascular disease in Asian diabetic patients?

    • Author(s)
      Sone H, et al.
    • Journal Title

      Diabetes Care 印刷中

    • Related Report
      2004 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi