| Project/Area Number |
16590896
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Osaka Medical College |
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Principal Investigator |
HANAFUSA Toshiaki Osaka Medical College, Faculty of Medicine, Professor, 医学部, 教授 (60164886)
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| Co-Investigator(Kenkyū-buntansha) |
IMAGAWA Akihisa Osaka Medical College, Faculty of Medicine, Assistant Professor, 医学部, 助手 (80373108)
TERASAKI Jungo Osaka Medical College, Faculty of Medicine, Assistant Professor, 医学部, 助手 (90351395)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | diabetes / enterovirus / VP1 / immunohistochemistry / fulminant / diabetes / IDDM / enterovirus / coxsackie virus / biopsy / immunohistochemistry / type 1 diabetes / viral infection |
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| Research Abstract |
1.Enterovirus antibodies in the sera of patients with fulminant type 1 diabetes.
We have investigated 19 patients with recent-onset fulminant type 1 diabetes, 18 patients with recent-onset typical type 1A diabetes, and 19 healthy controls. IgM, IgG, and IgA subclasses of antibodies to enterovirus were determined by ELISA. IgA antibody titres to enterovirus were significantly higher in fulminant type 1 diabetes than in typical type 1A diabetes (p=0.033) and controls (p=0.0003). IgM antibodies to enterovirus were not detected in any subject. In conclusion, high titres of enterovirus IgA-antibodies in serum suggest recurrent enterovirus infections in fulminant type 1 diabetic patients indicating higher susceptibility to enteroviral infections among them. Such infections may have pathogenetic importance for the triggering of fulminant type 1 diabetes.
2.Identification of enterovirus-derived antigen in autopsy specimens with fulminant type 1 diabetes
We analyzed an autopsy case of fulminant type 1 diabetes died soon after the onset of overt diabetes by using immunohistochemical methods. We found positive staining of enterovirus capsid antigen, VP1, in the specimens. It was strongly stained in exocrine pancreas and weakly in islet area with remained beta cells. These findings suggest that enterovirus targeted to human pancreas at least in part of fulminant diabetes.
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