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The establishment of cell therapy using regenerated-adrenocortical cells tranfected by Ad4BP/SF-1 cDNA

Research Project

Project/Area Number 16590908
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Endocrinology
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

OKABE Taijiro  Kyushu University, University Hospital, Research Associate, 大学病院, 助手 (40264030)

Co-Investigator(Kenkyū-buntansha) YANASE Toshihiko  Kyushu University, Graduate School of Medical Science, Associate Professor, 大学院・医学研究院, 助教授 (30239818)
GOTO Kiminobu  Kyushu University, University Hospital, Research Associate, 大学病院, 助手 (90284512)
NOMURA Masatoshi  Kyushu University, University Hospital, Research Associate, 大学病院, 助手 (30315080)
NAAWTA Hajime  Kyushu University, Graduate School of Medical Science, Professor, 大学院・医学研究院, 教授 (10038820)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordsadrenal gland / regeneration / steroid hormone / bone marrow / tissue stem cell / cell therapy
Research Abstract

It was found that long-term culture of adherent murine bone marrow cells in Dexter culture medium provided us the easy way of producing large quantity of undifferentiated mesenchymal stem cell-like cells. It was also found that transfection of exogenous Ad4BP/SF-1 cDNA transformed these bone marrow-derived cells into ACTH-responsive steroidogenic cells. These cells also expressed the several genes of steroidogenic enzymes and continued to produce several steroids for more than 120 days. In order to analyze the in vivo effect of these steroidogenic cells, model mouse of adrenal failure was produced by removing both adrenal glands sequentially from scid mouse. Transplantation of the steroidogenic cells into subcapsular space of the kidney after removing one adrenal gland rescued the mice and prolonged the survival. These findings may lead to cell therapy using regenerated steroidogenic cells in the near future. This study is also significant in showing the possibility that bone marrow cells could differentiate into several lineage by changing the differentiation program of the cell, not by cell fusion.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (5 results)

All 2004

All Journal Article (3 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] SF-1/Ad4BP transforms primary long-cultured bone marrow cells into ACTH-responsive steroidogenic cells2004

    • Author(s)
      Shigeki Gondo et al.
    • Journal Title

      Genes To Cells 9

      Pages: 1239-1247

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] SF-1/Ad4BP transforms primary long-term cultured bone marrow cells into ACTH-responsive steroidogenic cells.2004

    • Author(s)
      Shigeki Gondo et al.
    • Journal Title

      Genes To Cells 9

      Pages: 1239-1247

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] SF-1/Ad4BP Transforms primary long-term cultured bone marrow cells into ACTH-responsive steroidogenic cells2004

    • Author(s)
      Shigeki Gondo et al.
    • Journal Title

      Genes to Cells 9

      Pages: 1239-1247

    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] 新規ステロイド生産細胞2004

    • Inventor(s)
      権藤重喜
    • Industrial Property Rights Holder
      権藤重喜ほか
    • Industrial Property Number
      2004-289709
    • Filing Date
      2004-10-01
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 新規ステロイド産生細胞2004

    • Inventor(s)
      権藤 重喜
    • Industrial Property Rights Holder
      権藤 重喜, 柳瀬 敏彦ほか
    • Industrial Property Number
      2004-289709
    • Filing Date
      2004-10-01
    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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