| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Research Abstract |
To clarify the regulatory mechanism for erythropoiesis by heme, we performed differential expression analysis between wild-type and heme-deficient erythroblasts, which had been prepared from wild-type and δ-aminolevulinate synthase (ALAS2)-null mouse ES cells, respectively. From 8737 clones on cDNA array, clones which were highly expressed in wild-type erythroblasts were selected first and their expression was evaluated further for the erythroid-lineage specificity, heme-dependent expression, and expression in hematopoietic tissues in vivo. The final 4 genes were considered as the newly identified erythroid-specific heme-regulated genes. These 4 genes were uncoupling protein 2 (UCP2), nucleolar spindle-associated protein (NuSAP), cellular nucleic acid binding protein (CNBP), and a novel acetyltransferase-like protein. These findings suggest that heme may regulate a wide variety of hitherto unrecognized genes, and further analysis of these genes may clarify their role in erythroid cell differentiation.
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