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Hematopoietic lineage determination by transcription factors.

Research Project

Project/Area Number 16590939
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionOsaka University

Principal Investigator

KITAJIMA Kenji  Osaka University, Graduate School of Medicine, Specially appointed Associate Professor, 医学系研究科, 特任助教授(常勤) (10346132)

Co-Investigator(Kenkyū-buntansha) NAKANO Toru  Osaka University, Graduate School of Frontier Biosciences, Professor, 生命機能研究科, 教授 (00172370)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsHematopoiesis / Differentiation / Embryonic stem cells / Transcription factor / Lineage switch / 血液 / 赤血球 / 巨核球 / マクロファージ / クロマチン構造 / 分子生物学
Research Abstract

GATA-2 is a zinc finger transcription factor essential for differentiation of immature hematopoietic cells. We analyzed the function of GATA-2 by a combined method of tetracycline-dependent conditional gene expression and in vitro hematopoietic differentiation from mouse embryonic stem (ES) cells using OP9 stroma cells (OP9 system). In the presence of macrophage colony-stimulating factor (M-CSF), the OP9 system induced macrophage differentiation. GATA-2 expression in this system inhibited macrophage differentiation and redirected the fate of hematopoietic differentiation to other hematopoietic lineages. GATA-2 expression commencing at day 5 or day 6 induced megakaryocytic or erythroid differentiation, respectively. Expression levels of PU.1,a hematopoietic transcription factor that interferes with GATA-2,appeared to play a critical role in differentiation to megakaryocytic or erythroid lineages. Transcription of PU.1 was affected by histone acetylation induced by binding of GATA-2 to the PU.1 promoter region. This study demonstrates that the function of GATA-2 is modified in a contextdependent manner by expression of PU.1, which in turn is regulated by GATA-2.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (15 results)

All 2006 2004 Other

All Journal Article (15 results)

  • [Journal Article] Multipotential differentiation ability of GATA-1 null erythroid committed cells.2006

    • Author(s)
      Kitajima K
    • Journal Title

      Genes & Development 20

      Pages: 654-659

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Redirecting differentiation of hematopoietic progenitors by a transcription factor GATA-2.2006

    • Author(s)
      Kitajima K
    • Journal Title

      Blood 107

      Pages: 1857-1863

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Differential effects of GATA-1 on proliferation and differentiation of erythroid lineage cells.2006

    • Author(s)
      Zheng J
    • Journal Title

      Blood 107

      Pages: 520-527

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Multpotential differentiation ability of GATA-1-null erythroid-committed cells.2006

    • Author(s)
      Kitajima K.
    • Journal Title

      Genes Dev 20

      Pages: 654-659

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Redirecting differentiation of hematopoietic progenitors by a transcription factor GATA-2.2006

    • Author(s)
      Kitajima K.
    • Journal Title

      Blood 107

      Pages: 1857-1863

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Differential effects of GATA-1 on proliferation and differentiation of erythroid lineage cells.2006

    • Author(s)
      Zheng J.
    • Journal Title

      Blood 107

      Pages: 520-527

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Differential effects of GATA-1 on proliferation and differentiation of erythroid lineage cells.2006

    • Author(s)
      Jie Zheng
    • Journal Title

      Blood 107

      Pages: 520-527

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Differentiation status dependent function of FOG-1.2004

    • Author(s)
      Tanaka M
    • Journal Title

      Genes to Cells 9

      Pages: 1213-1226

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cross talk between retinoic acid signaling and transcription factor GATA-2.2004

    • Author(s)
      Tsuzuki S
    • Journal Title

      Mol.Cell.Biol. 24

      Pages: 6824-6836

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Differentiation status dependent function of FOG-1.2004

    • Author(s)
      Tanaka M.
    • Journal Title

      Genes Cells 9

      Pages: 1213-1226

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cross talk between retinoic acid signaling and transcription factor GATA-2.2004

    • Author(s)
      Tsuzuki S.
    • Journal Title

      Mol Cell Biol 24

      Pages: 6824-6836

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Differentiation status dependent function of FOG-1.2004

    • Author(s)
      Tanaka M
    • Journal Title

      Genes Cells 9

      Pages: 1213-1226

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cross talk between retinoic acid signaling and transcription factor GATA-2.2004

    • Author(s)
      Tsuzuki S
    • Journal Title

      Mol Cell Biol 24

      Pages: 6824-6836

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Multipotential differentiation ability of GATA-1 null erythroid committed cells.

    • Author(s)
      Kenji Kitajima
    • Journal Title

      Genes & Development in press

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Redirecting differentiation of hematopoietic progenitors by a transcription factor GATA-2.

    • Author(s)
      Kenji Kitajima
    • Journal Title

      Blood in press

    • Related Report
      2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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