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A challenge for long-term human lymphohemopoietic reconstitution in mice with normal immunity

Research Project

Project/Area Number 16590957
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

KIMURA Takafumi  Kyoto Prefectural University of Medicine, Hygiene, Assistant Professor, 医学研究科, 助手 (30275193)

Co-Investigator(Kenkyū-buntansha) SONODA Yoshiaki  Kansai Medical University, Hygiene, Professor, 医学部, 教授 (60206688)
IKEHARA Susumu  Kansai Medical University, Pathology, Professor, 医学部, 教授 (90108986)
KAKAZU Naoki  Kyoto Prefectural University of Medicine, Hygiene, Assistant Professor, 医学研究科, 助手 (20264757)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordshematopoietic stem cell / xenotransplantation / flow cytometry
Research Abstract

We transplanted human bone marrow cells into a total of 108 recipient mice with normal immunity (C57BL/6 and BDF-1), aiming at long-term human lymphohemopoietic reconstitution. Unexpectedly, most recipient mice receiving transplants (intra-bone marrow injection) of human marrow cells showed human cell repopulation for more than 18 month. The percentage of human cells in mouse bone marrow was confirmed to be 2 to 5 % by flow cytometric analysis. Moreover, no mice bearing human hemopoietic cells had obvious GvHD reaction, suggesting the usefulness of IBMI for prevention of lethal allogeneic immune responses.
We also found and reported the distinct and profound stem cell property of human CD34-negative (CD34^-) cells as compared with CD34^+ progenitors isolated from human umbilical cord blood using a NOD/SCID mice as recipients.
In addition, we reported impaired stem cell capacity, such as proliferation and differentiation potentials, of human CD34^+ cells isolated by clinical grade-immunomagnetic beads systems.
In the next step, we will try to improve the method for xenotransplants in order to get higher human cell repopulation in mice with normal immunity.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (9 results)

All 2005 2004

All Journal Article (9 results)

  • [Journal Article] Identification and hematopoietic potential of CD45^- clonal cells with very immature phenotype (CD45^-CD34^-CD38^-Lin^-)in patients with myelodysplastic syndromes.2005

    • Author(s)
      Ogata K, Satoh C, Tachibana M, Hyodo H, Tamura H, Dan K, Kimura T, Sonoda Y, Tsuji T.
    • Journal Title

      Stem Cells 23

      Pages: 619-630

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Identification and hematopoietic potential of CD45^- clonal cells with very immature phenotype (CD45^-CD34^-CD38^-Lin^-) in patients with myelodysplastic syndromes.2005

    • Author(s)
      Ogata K, Satoh C, Tachibana M, Hyodo H, Tamura H, Dan K, Kimura T, Sonoda Y, Tsuji T.
    • Journal Title

      Stem Cells 23

      Pages: 619-630

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Preferential expression of the vasoactive intestinal peptide (VIP) receptor VPAC1 in human cord blood-derived CD34^+CD38^- cells : possible role of VIP as a growth-promoling factor for hematopoietic stem/ progenitor cells.2004

    • Author(s)
      M, Kimura T, Kishimoto Y, Tatekawa T, Baba Y, Nishizaki T, Matsuzaki N, Taniguchi Y, Yoshihara S, Ikegame K, Shirakata T, Nishida S, Masuda T, Hosen N, Tsuboi A, Oji Y, Oka Y, Ogawa H, Sonoda Y, Sugiyama H, Kawase I, Soma T.
    • Journal Title

      Leikemia 18

      Pages: 912-921

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The sphingosine 1-phosphate agonist FTY720 supports CXCR4-dependent migration and bone marrow homing of human CD34^+ progenitor cells.2004

    • Author(s)
      Kimura T, Boehmler AM, Seitz G, Kuci S, Wiesner T, Brinkmann V, Kanz L, Mohle R.
    • Journal Title

      Blood 103

      Pages: 4478-4486

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Preferential expression of the vasoactive intestinal peptide (VIP) receptor VPAC1 in human cord blood-derived CD34^+CD38^-cells : possible role of VIP as a growth- promoting factor for hematopoietic stem/ progenitor cells.2004

    • Author(s)
      Kawakami M, Kimura T, Kishimoto Y, Tatekawa T, Baba Y, Nishizaki T, Matsuzaki N, Taniguchi Y, Yoshihara S, Ikegame K, Shirakata T, Nishida S, Masuda T, Hosen N, Tsuboi A, Oji Y, Oka Y, Ogawa H, Sonoda Y, Sugiyama H, Kawase I, Soma T.
    • Journal Title

      Leukemia 18

      Pages: 912-921

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The sphingosine 1-phosphate agonist FTY720 supports CXCR4-dependent migration and bone marrow homing of human CD34^+ progenitor cells.2004

    • Author(s)
      Kimura T, Boehmler AM, Seitz G, Kuci S, Wiesner T, Brinkmann V, Kanz L, MOhle R.
    • Journal Title

      Blood 103

      Pages: 4478-4486

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The sphingosine 1-phosphate agonist FTY720 supports CXCR4-dependent migration and bone marrow homing of human CD34^+ progenitor cells.2004

    • Author(s)
      Kimura T.Boehmler AM, Seitz G, Kuci S, Wiesner T, Brinkmann V, Kanz L, Mohle R.
    • Journal Title

      Blood 103

      Pages: 4478-4486

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Proliferative and migratory potentials of human cord blood-derived CD34^- severe combined immunodeficiency repopulating cells that retain secondary reconstituting capacity.2004

    • Author(s)
      Kimura T, Wang J, Matsui K, Imai S, Yokoyama S, Nishikawa M, Ikehara S, Sonoda Y.
    • Journal Title

      Int.J.Hematol. 79

      Pages: 328-333

    • NAID

      10013081994

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Impaired stem cell function of CD34^+ cells selected by two different immunomagnetic beads systems.2004

    • Author(s)
      Kimura T, Minamiguchi H, Wang J, kaneko H, Nakagawa H, Fujii H, Sonoda Y.
    • Journal Title

      Leukemia 18

      Pages: 566-574

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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