| Project/Area Number |
16590975
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | Chiba University |
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Principal Investigator |
KOHNO Yoichi Chiba University, Graduate School of Medicine, Professor, 大学院医学研究院, 教授 (60161882)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMOJO Naoki Chiba University, Graduate School of Medicine, Associate Professor, 大学院医学研究院, 助教授 (40221303)
TOMITA Minako Chiba University, Graduate School of Medicine, Assistant Professor, 大学院医学研究院, 助手 (60344991)
FUJII Katsunori Chiba University, Graduate School of Medicine, Assistant Professor, 大学院医学研究院, 助手 (70344992)
ISHIWADA Naruhiko Chiba University, Chiba University Hospital, Associate Professor, 医学部附属病院, 講師 (30344980)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Respiratory syncytial virus / CD14 / genetic polymorphism / bronchiolitis / tumor necrosis factor alpha / TLR4 |
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| Research Abstract |
The contribution of genetic polymorphisms of Toll-like receptor (TLR) 4 and CD 14, which recognize RSV in the innate immune response, to RSV bronchiolitis has not yet been clarified in the Japanese population.
We genotyped two TLR4 mutations, Asp299Gly and Thr399Ile, and two single nucleotide polymorphisms of CD 14, -550 C/T and -159 C/T, in 54 children with RSV bronchiolitis and 203 control subjects. CD14 SNP and the serum soluble CD14 (sCD14) levels were examined in 67 cord blood specimens and 73 six-year-old children.
We did not find any TLR4 mutations. The frequency of the CC genotype and C allele of CD14 -550 C/T in children with RSV bronchiolitis were significantly higher than those in the controls. The serum sCDl4 level was significantly higher in the CC genotype of CD14 -550 C/T than that in the CT and TT genotype.
CD14 -550 C/T, which is related to the serum sCD14 level, is associated with the development of RSV bronchiolitis in the Japanese population. The results indicate that different genetic factors contribute to the development of RSV bronchiolitis between ethnics groups.
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