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Examination of signaling pathways to determine the process of proliferation, apoptosis and cellular differentiation of fibroblast-like synovial cells

Research Project

Project/Area Number 16590987
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 膠原病・アレルギー・感染症内科学
Research InstitutionNagasaki University

Principal Investigator

KAWAKAMI Atsushi  Nagasaki University, Hospital of Medicine and Dentistry, Lecturer, 医学部・歯学部附属病院, 講師 (90325639)

Co-Investigator(Kenkyū-buntansha) ORIGUCHI Tomoki  Nagasaki University, Graduate School of Biomedical Sciences, associate professor, 大学院・医歯薬学総合研究科, 助教授 (90295105)
IDA Hiroaki  Nagasaki University, Hospital of Medicine and Dentistry, assistant, 医学部・歯学部附属病院, 助手 (60363496)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsfibroblast-like synovial cells / rheumatoid arthritis (RA) / cytokine / IFN-γ / cellular differentiation / JAK / STAT / TRAIL-mediated apoptosis / apoptosis-related molecule / 滑膜線維芽細胞(FLS) / アポトーシス / Akt / P13-K / CaMKII / FLS / 分化 / TRAIL依存性アポトーシス / 蛋白合成 / caspase-8
Research Abstract

Hyperplasia of fibroblast-like synovial cells is a characteristic feature in patients with rheumatoid arthritis (RA). Inflammatory cytokines as well as growth factors, expressed in situ of rheumatoid synovial tissues, are suggested to induce "apoptosis-resistant phenotype" of fibroblast-like synovial cells. In addition, these humoral factors may involve in the differentiation process of fibroblast-like synovial cells ; a newly denoted function of the cells. We have examined how cytokines modulate the process of both apoptosis sensitivity and cellular differentiation. As for the effect of IFN-γ toward fibroblast-like synovial cells, IFN-γ significantly suppressed the expression of PPAR-γ and C/EBP nuclear activity, and thus inhibited PPAR-γ ligand-induced adipocyte-like cell differentiation from fibroblast-like synovial cells. Furthermore, IFN-γ treatment markedly phosphorylated STAT proteins through JAK, leading to induce "apoptosis-resistant phenotype" toward TRAIL. Our data suggest that IFN-γ induces the two phenomena ; "inhibition of cellular differentiation" and "induction of apoptosis-resistant phenotype" through distinct signaling cascades, however, further studies, including the elucidation of signal cross-talks of kinase cascades in the above two phenomena, should be necessary to approach the molecular mechanism.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (39 results)

All 2006 2005 2004 Other

All Journal Article (39 results)

  • [Journal Article] Significant inhibition of TRAIL-mediated fibroblast-like synovial cell apoptosis by IFN-gamma through JAK/STAT pathway by translational regulation.2006

    • Author(s)
      Tamai M, Kawakami A (2番目, 他9名)
    • Journal Title

      J.Lab.Clin.Med. 147

      Pages: 182-190

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of Peripheral Natural Killer Cells in Primary Sjogren's Syndrome : Impaired NK Cell Activity and Low NK Cell Number.2006

    • Author(s)
      Izumi Y, Kawakami A (12番目, 他12名)
    • Journal Title

      J.Lab.Clin.Med. (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 病態形成とアポトーシス2006

    • Author(s)
      川上 純, 江口勝美(筆頭著者, 他3名)
    • Journal Title

      日本臨床 63

      Pages: 100-105

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Detection of apoptosis-specific autoantoantibodies directed against granzyme B-induced clevage fragments of the SS-B(La)autoantigen in serafrom patients with primary Sjogren's syndrome.2005

    • Author(s)
      Huang M, Kawakami A (12番目, 他14名)
    • Journal Title

      Clin.Exp.Immunol. 142

      Pages: 148-152

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Detection of the soluble form of Fas ligand (sFasL) and sFas in the saliva from patients with Sjogren's syndrome.2005

    • Author(s)
      Nakamura H, Kawakami A (2番目, 他7名)
    • Journal Title

      Clin.Exp.Rheumatol. 23

      Pages: 915-915

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Across-talk between RNA splicing and signaling pathway aiters Fas gene expression at post-transcriptional level : Alternative splicing of Fas mRNA in the leukemic U937 cells.2005

    • Author(s)
      Aratake K, Kawakami A (twelfth, other eleventh coauthors)
    • Journal Title

      J.Lab.Clin.Med. 146

      Pages: 184-191

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Detection of apoptosis-specific autoantoantibodies directed against granzyme B-induced clevage fragments of the SS-B(La)autoantigen in sera from patients with primary Sjogren's syndrome.2005

    • Author(s)
      Huang M, Kawakami A (twelfth, other fourteenth coauthors)
    • Journal Title

      Clin.Exp.Immunol. 142

      Pages: 148-152

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Detection of the soluble form of Fas ligand (sFasL) and sFas in the saliva from patients with Sjogren's syndrome.2005

    • Author(s)
      Nakamura H, Kawakami A (second, other seventh coauthors)
    • Journal Title

      Clin.Exp.Rheumatol. 23

      Pages: 915-915

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Significant inhibition of TRAIL-mediated fibmblast-like synovial cell apoptosis by IFN-gamma through JAK/STAT pathway by translational regulation.2005

    • Author(s)
      Tamai M, Kawakami A (second, other ninth coauthors)
    • Journal Title

      J.Lab.Clin.Med. 147

      Pages: 182-190

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of Peripheral Natural Killer Cells in Primary Sjogren's Syndrome : Impaired NK Cell Activity and Low NK Cell Number.2005

    • Author(s)
      Izumi Y, Kawakami A (twelfth, other twelveth coauthors)
    • Journal Title

      J.Lab.Clin.Med. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Detection of the soluble form of Fas ligand (sFasL) and sFas in the saliva from patients with Sjogren's syndrome.2005

    • Author(s)
      Nakamura H, Kawakami A(他7名, 2番目)
    • Journal Title

      Clin Exp Rheumatol 23(6)

      Pages: 915-915

    • Related Report
      2005 Annual Research Report
  • [Journal Article] IFN-gamma/JAK/STAT pathway-induced inhibition of DR4 and DR5 expression on endothelial cells is cancelled by cycloheximide-sensitive mechanism : novel finding of cycloheximide-regulating death receptor expression.2005

    • Author(s)
      Tanaka F, Kawakami A(他11名, 2番目)
    • Journal Title

      Int J Mol Med 15(5)

      Pages: 833-839

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Regulation of alternative splicing of caspase-2 through an intracellular signaling pathway in response to pro-apoptotic stimuli.2005

    • Author(s)
      Iwanaga N, Kawakami A(他11名, 11番目)
    • Journal Title

      J Lab Clin Med 145(2)

      Pages: 105-110

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Detection of apoptosis-specific autoantibodies directed against granzyme B-induced cleavage fragments of the SS-B (La) autoantigen in sera from patients with primary Sjogren's syndrome.2005

    • Author(s)
      Mingguo H, Kawakami A(他16名, 13番目)
    • Journal Title

      Clin Exp Immunol 142(1)

      Pages: 148-152

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A cross-talk between RNA splicing and signaling pathway alters Fas gene expression at post-transcriptional level: alternative splicing of Fas mRNA in the leukemic U937 cells.2005

    • Author(s)
      Aratake K, Kawakami A(他11名, 11番目)
    • Journal Title

      J Lab Clin Med 146(3)

      Pages: 184-191

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 関節リウマチ -成因研究から治療の新時代- 病態形成とアポトーシス2005

    • Author(s)
      川上 純(筆頭著者, 他4名)
    • Journal Title

      日本臨床 63(1)

      Pages: 100-105

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Suppressive effect of leflunomide metabolite (A77 1726) on metalloproteinase production in IL-1β stimulated rheumatoid synovial fibroblasts.2004

    • Author(s)
      Migita K, Kawakami A (8番目, 他9名)
    • Journal Title

      Clin.Exp.Immunol 137

      Pages: 612-616

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Osteoprotegerin(OPG) acts as an endogenous decoy receptor in tumour necrosis factor-related apoptosis-inducing ligand(TRAIL)-mediated apoptosis of fibroblast-like synovial cells.2004

    • Author(s)
      Miyashita T, Kawakami A (2番目, 他10名)
    • Journal Title

      Clin.Exp.Immunol 137

      Pages: 430-436

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Antiarrhythmic Amiodarone Mediates Apoptotic Cell Death of HepG2 Hepatoblastoma Cells through the Mitochondrial Pathway.2004

    • Author(s)
      Isomoto S, Kawakami A (2番目, 他3名)
    • Journal Title

      Acta.Med.Nagasaki 49

      Pages: 13-17

    • NAID

      110000939240

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cytokines regulate fiblobrast-like synovial cell differentiation to adipocyte-like cells.2004

    • Author(s)
      Yamasaki S, Kawakami A (3番目, 他7名)
    • Journal Title

      Rheumatology 43

      Pages: 448-452

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Interleukin-18 induces serum amyloid A (SAA) protein production from rheumatoid synovial fibroblasts.2004

    • Author(s)
      Tanaka F, Kawakami A (6番目, 他5名)
    • Journal Title

      Life Sciences 74

      Pages: 1671-1679

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] NF-κB、MAPK制御による抗炎症療法2004

    • Author(s)
      川上 純, 江口勝美(筆頭著者, 他4名)
    • Journal Title

      医学のあゆみ 74

      Pages: 386-390

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Suppressive effect of leflunomide metabolite (A77 1726) on metalloproteinase production in IL-1 β stimulated rheumatoid synovial fibroblasts.2004

    • Author(s)
      Migita K, Kawakami A (eighth, other 9 coauthors)
    • Journal Title

      Clin.Exp.Immunol. 137

      Pages: 612-616

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Osteoprotegerm (OPG) acts as an endogenous decoy receptor in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of fibroblast-like synovial cells.2004

    • Author(s)
      Miyashita T, Kawakami A (second, other 10 coauthors)
    • Journal Title

      Clin.Exp.Immunol. 137

      Pages: 430-436

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Antiarrhythmic Amiodarone Mediates Apoptotic Cell Death of HepG2 Hepatoblastoma Cells through the Mitochondrial Pathway.2004

    • Author(s)
      Isomoto S, Kawakami A (second, other 3 coauthors)
    • Journal Title

      Acta.Med.Nagasaki. 49

      Pages: 13-17

    • NAID

      110000939240

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cytokines regulate fiblobrast-like synovial cell differentiation to adipocyte-like cells.2004

    • Author(s)
      Yamasaki S, Kawakami A (third, other 7 coauthors)
    • Journal Title

      Rheumatology. 43

      Pages: 448-452

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Interleukin-18 induces serum amyloid A (SAA) protein production from rheumatoid synovial fibroblasts.2004

    • Author(s)
      Tanaka F, Kawakami A (sixth, other 5 coauthors)
    • Journal Title

      Life Sciences 74

      Pages: 1671-1679

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Osteoprotegerin (OPG) acts as an endogenous decoy receptor in tumour necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of fibroblast-like synovial cells.2004

    • Author(s)
      Miyashita T, Kawakami A(2番目, 他10名)
    • Journal Title

      Clin Exp Immunool 137

      Pages: 430-436

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Nitric oxide acts on the mitochondria and protects human endothelial cells from apoptosis.2004

    • Author(s)
      Hida A, Kawakami A(2番目, 他12名)
    • Journal Title

      J Lab Clin Med 144

      Pages: 148-155

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Cytokines regulate fibrolastlike synovial cell differentiation to adipocyte-like cells.2004

    • Author(s)
      Yamasaki F, Kawakami A(3番目, 他7名)
    • Journal Title

      Rheumatology 43

      Pages: 448-452

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Anti-apoptogenic function of TGFbeta 1 for human synovial cells : TGFbeta 1 protects cultured synovial cells from mitochondrial perturbation induced-by several apoptogenic stimuli.2004

    • Author(s)
      Kawakami A(筆頭著者, 他14名)
    • Journal Title

      Ann Rheum Dis 63

      Pages: 95-97

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Suppressive effect of leflunomide metabolite (A77 1726) on metalloproteinase production in IL-1beta stimulated rheumatoid synovial fibroblasts.2004

    • Author(s)
      Migita K, Kawakami A(8番目, 他10名)
    • Journal Title

      Clin Exp Immunool 137

      Pages: 612-616

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A novel mutation (T611) in the gene encoding tumour necrosis factor receptor superfamily 1A (TNFRSF1A) in a Japanese patient with tumor necrosis factor receptor-associated periodic syndrome (TRAPS) associated with systemic lupus erythematosus.2004

    • Author(s)
      Ida H, Kawakami A(10番目, 他10名)
    • Journal Title

      Rheumatology 48

      Pages: 1292-299

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The role of IL-18 in the modulation of matrix metalloproterinases and migration of human natural killer (NK) cells.2004

    • Author(s)
      Ishida Y, Kawakami A(6番目, 他4名)
    • Journal Title

      FEBS Lett 569

      Pages: 156-160

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Interleukin-18 induces serum amyloid A (SAA) protein production from rheumatoid synovial fiblasts.2004

    • Author(s)
      Tanaka F, Kawakami A(6番目, 他5名)
    • Journal Title

      Life Sci 74

      Pages: 1671-1679

    • Related Report
      2004 Annual Research Report
  • [Journal Article] TRAIL欠損マウスにおける自己免疫疾患の発症2004

    • Author(s)
      川上 純(筆頭著者, 他4名)
    • Journal Title

      臨床免疫 41

      Pages: 73-77

    • NAID

      40006099436

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 関節リウマチの成因と病態生理概論的事項病態形成とアポトーシス2004

    • Author(s)
      川上 純(筆頭著者, 他4名)
    • Journal Title

      日本臨床 63

      Pages: 100-105

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Significant inhibition of TRAIL-mediated fibroblast-like synovial cell apoptosis by IFN-γ through JAK/STAT pathway by translational regulation.

    • Author(s)
      Tamai M, Kawakami A(他9名, 2番目)
    • Journal Title

      J Lab Clin Med (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Significant inhibition of TRAIL-mediated fibroblast-like synovial cell apoptosis by IFN-γ through JAK/STAT pathway by translational regulation.

    • Author(s)
      Tamai M, Kawakami A(2番目, 他12名)
    • Journal Title

      Int J Mol Med (In press)

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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