| Co-Investigator(Kenkyū-buntansha) |
SATO Ryugo Oita University, Faculty of medicine, Research assistant, 医学部, 助手 (00325705)
MURAKAMI Kazunari Oita University, Faculty of medicine, Assistant Professor, 医学部, 助教授 (00239485)
GOTOH Kazuyo Oita University, Institute of Scientific Research, Research assistant, 総合科学研究支援センター, 助手 (30381031)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Research Abstract |
To investigate the possibility of an interaction between two ubiquitous human pathogens, Helicobacter pylori (H.pylori) and Epstein-Barr virus (EBV), the effect of monochloramine, locally produced by H.pylori infection, on gastric epithelium latently infected with EBV was examined, by assessing the induction of EBV lytic infection. AGS cells harbouring latently infected EBV were used as the indicator of lytic change caused by monochloramine treatment. Lytic infection, determined by morphological change and EA-D antigen expression, occurred immediately after treatment with in vitro-synthesized monochloramine. Analysis of EBV infection in human gastric tissue revealed that out of 48 H.-pylori-positive patients, 24 were positive for EBER-1, and 18 and 13 were positive for EBNA1 and LMP-a antigen, respectively. The results suggest that H.pylori-associated monochloramine induces EBV lytic conversion in gastric epithelium latently infected with EBV.
Helicobacter pylori is a gram-negative bacterium that colonizes the human stomach and causes chronic gastric diseases. It is recognized as the etiologic agent of atrophic gastritis, peptic ulcer, intestinal metaplasia, MALToma and gastric cancer. Various pathogenic proteins of H.pylori have been reported, such as VacA, CagA, LPS, HSP60 and unease. Dendritic cells (DCs) are potent antigen-presenting cells that initiate acquired immune responses. Previously HP-MP1 has been reported as a new proinflammatory mediator in stomach infected with H.pylori and HP0596 gene is homologous to HP-MP1. We designated the HP0596 gene as the TNF-a inducing protein (Tipa) and Tipa was present in the culture medium of H.pylori. We also reported that Monomer Tipa is 19kDa-sized protein located in H.pylori membrane fraction and forms homodimer structure due to two cysteine residues in N-terminal site. In this study, we report here an immunomodulatory effect of novel Tipa protein of H.pylori to DCs.
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